Experiment: L-Histidine
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS35490 and BPHYT_RS35495 are separated by 346 nucleotides BPHYT_RS35495 and BPHYT_RS35500 are separated by 50 nucleotides BPHYT_RS35500 and BPHYT_RS35505 are separated by 82 nucleotides BPHYT_RS35505 and BPHYT_RS35510 are separated by 55 nucleotides
BPHYT_RS35490: BPHYT_RS35490 - hypothetical protein, at 3,563,856 to 3,564,416
_RS35490
BPHYT_RS35495: BPHYT_RS35495 - nucleotide pyrophosphohydrolase, at 3,564,763 to 3,565,134
_RS35495
BPHYT_RS35500: BPHYT_RS35500 - major facilitator transporter, at 3,565,185 to 3,566,621
_RS35500
BPHYT_RS35505: BPHYT_RS35505 - short-chain dehydrogenase, at 3,566,704 to 3,567,474
_RS35505
BPHYT_RS35510: BPHYT_RS35510 - hydrolase, at 3,567,530 to 3,568,384
_RS35510
Position (kb)
3565
3566
3567 Strain fitness (log2 ratio)
-2
-1
0
1 at 3564.262 kb on + strand, within BPHYT_RS35490 at 3564.472 kb on + strand at 3564.714 kb on - strand at 3564.998 kb on - strand, within BPHYT_RS35495 at 3565.362 kb on + strand, within BPHYT_RS35500 at 3565.363 kb on - strand, within BPHYT_RS35500 at 3565.363 kb on - strand, within BPHYT_RS35500 at 3565.881 kb on - strand, within BPHYT_RS35500 at 3566.653 kb on - strand at 3566.901 kb on - strand, within BPHYT_RS35505 at 3567.187 kb on + strand, within BPHYT_RS35505 at 3567.187 kb on + strand, within BPHYT_RS35505
Per-strain Table
Position Strand Gene LocusTag Fraction L-Histidine remove 3,564,262 + BPHYT_RS35490 0.72 -0.1 3,564,472 + +0.3 3,564,714 - +0.0 3,564,998 - BPHYT_RS35495 0.63 +0.4 3,565,362 + BPHYT_RS35500 0.12 -0.4 3,565,363 - BPHYT_RS35500 0.12 -2.0 3,565,363 - BPHYT_RS35500 0.12 -0.6 3,565,881 - BPHYT_RS35500 0.48 -1.3 3,566,653 - +0.6 3,566,901 - BPHYT_RS35505 0.26 +0.2 3,567,187 + BPHYT_RS35505 0.63 +0.2 3,567,187 + BPHYT_RS35505 0.63 -0.4
Or see this region's nucleotide sequence