Experiment: L-Histidine
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS34940 and BPHYT_RS34945 overlap by 8 nucleotides BPHYT_RS34945 and BPHYT_RS34950 are separated by 26 nucleotides BPHYT_RS34950 and BPHYT_RS34955 are separated by 45 nucleotides BPHYT_RS34955 and BPHYT_RS34960 are separated by 0 nucleotides
BPHYT_RS34940: BPHYT_RS34940 - 3-phenylpropionate dioxygenase, at 3,439,366 to 3,440,460
_RS34940
BPHYT_RS34945: BPHYT_RS34945 - 3-ketoacyl-ACP reductase, at 3,440,453 to 3,441,220
_RS34945
BPHYT_RS34950: BPHYT_RS34950 - cupin, at 3,441,247 to 3,441,774
_RS34950
BPHYT_RS34955: BPHYT_RS34955 - alpha/beta hydrolase, at 3,441,820 to 3,442,710
_RS34955
BPHYT_RS34960: BPHYT_RS34960 - IclR family transcriptional regulator, at 3,442,711 to 3,443,595
_RS34960
Position (kb) 3441
3442 Strain fitness (log2 ratio) -1
0
1
2 at 3440.459 kb on - strand at 3440.643 kb on - strand, within BPHYT_RS34945 at 3440.937 kb on - strand, within BPHYT_RS34945 at 3441.084 kb on - strand, within BPHYT_RS34945 at 3441.531 kb on + strand, within BPHYT_RS34950 at 3441.724 kb on - strand at 3441.724 kb on - strand at 3441.841 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Histidine remove 3,440,459 - -1.0 3,440,643 - BPHYT_RS34945 0.25 +2.0 3,440,937 - BPHYT_RS34945 0.63 -0.3 3,441,084 - BPHYT_RS34945 0.82 +1.0 3,441,531 + BPHYT_RS34950 0.54 +0.6 3,441,724 - +1.3 3,441,724 - -0.2 3,441,841 - -0.3
Or see this region's nucleotide sequence