Experiment: L-Histidine
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS04905 and BPHYT_RS04910 are separated by 207 nucleotides BPHYT_RS04910 and BPHYT_RS04915 are separated by 210 nucleotides BPHYT_RS04915 and BPHYT_RS04920 are separated by 192 nucleotides BPHYT_RS04920 and BPHYT_RS04925 are separated by 34 nucleotides
BPHYT_RS04905: BPHYT_RS04905 - quinol oxidase, at 1,128,367 to 1,128,783
_RS04905
BPHYT_RS04910: BPHYT_RS04910 - phenylacetic acid degradation protein, at 1,128,991 to 1,129,410
_RS04910
BPHYT_RS04915: BPHYT_RS04915 - cobyrinic acid a,c-diamide synthase, at 1,129,621 to 1,130,259
_RS04915
BPHYT_RS04920: BPHYT_RS04920 - aspartate 1-decarboxylase, at 1,130,452 to 1,130,838
_RS04920
BPHYT_RS04925: BPHYT_RS04925 - pantothenate synthetase, at 1,130,873 to 1,131,706
_RS04925
Position (kb)
1129
1130
1131 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1128.896 kb on - strand at 1128.996 kb on - strand at 1129.012 kb on - strand at 1129.012 kb on - strand at 1129.067 kb on - strand, within BPHYT_RS04910 at 1129.458 kb on + strand at 1129.458 kb on + strand at 1129.459 kb on - strand at 1129.459 kb on - strand at 1129.499 kb on + strand at 1129.499 kb on + strand at 1129.572 kb on + strand at 1129.572 kb on + strand at 1129.693 kb on + strand, within BPHYT_RS04915 at 1129.836 kb on + strand, within BPHYT_RS04915 at 1129.837 kb on - strand, within BPHYT_RS04915 at 1129.918 kb on - strand, within BPHYT_RS04915 at 1129.954 kb on - strand, within BPHYT_RS04915 at 1130.116 kb on + strand, within BPHYT_RS04915 at 1130.137 kb on + strand, within BPHYT_RS04915 at 1130.137 kb on + strand, within BPHYT_RS04915 at 1130.138 kb on - strand, within BPHYT_RS04915 at 1130.138 kb on - strand, within BPHYT_RS04915 at 1130.314 kb on + strand at 1130.315 kb on - strand at 1130.315 kb on - strand at 1130.363 kb on + strand at 1130.419 kb on - strand at 1130.666 kb on + strand, within BPHYT_RS04920 at 1130.667 kb on - strand, within BPHYT_RS04920 at 1130.775 kb on - strand, within BPHYT_RS04920
Per-strain Table
Position Strand Gene LocusTag Fraction L-Histidine remove 1,128,896 - -0.2 1,128,996 - -0.6 1,129,012 - -1.0 1,129,012 - +0.6 1,129,067 - BPHYT_RS04910 0.18 +0.0 1,129,458 + +0.3 1,129,458 + -0.1 1,129,459 - -0.1 1,129,459 - -0.5 1,129,499 + -0.1 1,129,499 + +1.3 1,129,572 + -0.0 1,129,572 + -0.0 1,129,693 + BPHYT_RS04915 0.11 +0.4 1,129,836 + BPHYT_RS04915 0.34 +0.1 1,129,837 - BPHYT_RS04915 0.34 -0.3 1,129,918 - BPHYT_RS04915 0.46 -1.2 1,129,954 - BPHYT_RS04915 0.52 +0.1 1,130,116 + BPHYT_RS04915 0.77 -0.2 1,130,137 + BPHYT_RS04915 0.81 -0.3 1,130,137 + BPHYT_RS04915 0.81 +0.6 1,130,138 - BPHYT_RS04915 0.81 -0.2 1,130,138 - BPHYT_RS04915 0.81 -1.1 1,130,314 + +0.8 1,130,315 - -0.5 1,130,315 - +0.1 1,130,363 + +2.1 1,130,419 - -1.4 1,130,666 + BPHYT_RS04920 0.55 -1.0 1,130,667 - BPHYT_RS04920 0.56 -2.0 1,130,775 - BPHYT_RS04920 0.83 -1.7
Or see this region's nucleotide sequence