Experiment: MoLS4 with L-Asparagine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt DVU1639 and DORF12455 are separated by 95 nucleotides DORF12455 and DORF12457 overlap by 23 nucleotides DORF12457 and DVU1641 are separated by 44 nucleotides DVU1641 and DORF12461 are separated by 20 nucleotides DORF12461 and DORF12465 are separated by 39 nucleotides DORF12465 and DORF12467 overlap by 14 nucleotides DORF12467 and DORF12469 are separated by 3 nucleotides DORF12469 and DVU1643 are separated by 53 nucleotides
DVU1639: DVU1639 - conserved domain protein (TIGR), at 1,719,951 to 1,720,946
DVU1639
DORF12455: DORF12455 - SEED: helix-turn-helix protein, CopG family, at 1,721,042 to 1,721,284
DORF12455
DORF12457: DORF12457 - SEED: protein of unknown function DUF497, at 1,721,262 to 1,721,534
DORF12457
DVU1641: DVU1641 - conserved hypothetical protein (TIGR), at 1,721,579 to 1,721,902
DVU1641
DORF12461: DORF12461 - SEED: HigA protein (antitoxin to HigB), at 1,721,923 to 1,722,225
DORF12461
DORF12465: DORF12465 - SEED: HigB toxin protein, at 1,722,265 to 1,722,582
DORF12465
DORF12467: DORF12467 - SEED: protein of unknown function DUF497, at 1,722,569 to 1,722,736
DORF12467
DORF12469: DORF12469 - no description, at 1,722,740 to 1,722,838
DORF12469
DVU1643: DVU1643 - conserved hypothetical protein, degenerate (TIGR), at 1,722,892 to 1,723,426
DVU1643
Position (kb)
1721
1722 Strain fitness (log2 ratio)
-2
-1
0
1 at 1720.596 kb on + strand, within DVU1639 at 1720.599 kb on - strand, within DVU1639 at 1720.604 kb on - strand, within DVU1639 at 1720.757 kb on - strand, within DVU1639 at 1720.757 kb on - strand, within DVU1639 at 1720.818 kb on + strand, within DVU1639 at 1720.823 kb on + strand, within DVU1639 at 1720.852 kb on - strand at 1720.906 kb on + strand at 1720.913 kb on + strand at 1720.943 kb on + strand at 1721.040 kb on + strand at 1721.114 kb on + strand, within DORF12455 at 1721.228 kb on + strand, within DORF12455 at 1721.597 kb on - strand at 1721.752 kb on + strand, within DVU1641 at 1721.855 kb on + strand, within DVU1641 at 1721.906 kb on - strand at 1721.914 kb on + strand at 1721.923 kb on - strand at 1721.923 kb on - strand at 1721.981 kb on + strand, within DORF12461 at 1721.981 kb on + strand, within DORF12461 at 1722.186 kb on - strand, within DORF12461 at 1722.205 kb on - strand at 1722.249 kb on - strand at 1722.250 kb on + strand at 1722.327 kb on - strand, within DORF12465 at 1722.438 kb on + strand, within DORF12465 at 1722.441 kb on - strand, within DORF12465 at 1722.483 kb on - strand, within DORF12465 at 1722.676 kb on - strand, within DORF12467 at 1722.698 kb on + strand, within DORF12467 at 1722.898 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction MoLS4 with L-Asparagine (N) remove 1,720,596 + DVU1639 0.65 +0.9 1,720,599 - DVU1639 0.65 -0.7 1,720,604 - DVU1639 0.66 -0.4 1,720,757 - DVU1639 0.81 -0.8 1,720,757 - DVU1639 0.81 -0.4 1,720,818 + DVU1639 0.87 +0.0 1,720,823 + DVU1639 0.88 -0.1 1,720,852 - -2.2 1,720,906 + +1.5 1,720,913 + +0.2 1,720,943 + +0.1 1,721,040 + +0.1 1,721,114 + DORF12455 0.30 -0.9 1,721,228 + DORF12455 0.77 +0.5 1,721,597 - +0.9 1,721,752 + DVU1641 0.53 -0.0 1,721,855 + DVU1641 0.85 -1.6 1,721,906 - +0.6 1,721,914 + +0.3 1,721,923 - -0.8 1,721,923 - -1.9 1,721,981 + DORF12461 0.19 -0.8 1,721,981 + DORF12461 0.19 -0.2 1,722,186 - DORF12461 0.87 +0.1 1,722,205 - +0.6 1,722,249 - +0.2 1,722,250 + -0.5 1,722,327 - DORF12465 0.19 -0.3 1,722,438 + DORF12465 0.54 -1.1 1,722,441 - DORF12465 0.55 +0.1 1,722,483 - DORF12465 0.69 -0.0 1,722,676 - DORF12467 0.64 +0.9 1,722,698 + DORF12467 0.77 -0.1 1,722,898 + -0.6
Or see this region's nucleotide sequence