Strain Fitness in Pseudomonas fluorescens SBW25-INTG around PFLU_RS19645

Experiment: Growth at pH7 and (C) D-Glucose

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPFLU_RS19635 and PFLU_RS19640 are separated by 94 nucleotidesPFLU_RS19640 and PFLU_RS19645 overlap by 4 nucleotidesPFLU_RS19645 and PFLU_RS19650 overlap by 4 nucleotidesPFLU_RS19650 and PFLU_RS19655 overlap by 4 nucleotides PFLU_RS19635: PFLU_RS19635 - SDR family oxidoreductase, at 4,445,510 to 4,446,262 _RS19635 PFLU_RS19640: PFLU_RS19640 - 5-oxoprolinase subunit PxpA, at 4,446,357 to 4,447,130 _RS19640 PFLU_RS19645: PFLU_RS19645 - hypothetical protein, at 4,447,127 to 4,447,519 _RS19645 PFLU_RS19650: PFLU_RS19650 - biotin-dependent carboxyltransferase family protein, at 4,447,516 to 4,448,490 _RS19650 PFLU_RS19655: PFLU_RS19655 - 5-oxoprolinase subunit PxpB, at 4,448,487 to 4,449,143 _RS19655 Position (kb) 4447 4448Strain fitness (log2 ratio) -4 -3 -2 -1 0 1 2 3at 4446.185 kb on + strand, within PFLU_RS19635at 4446.185 kb on + strand, within PFLU_RS19635at 4446.185 kb on + strand, within PFLU_RS19635at 4446.300 kb on + strandat 4446.358 kb on + strandat 4446.359 kb on - strandat 4446.389 kb on + strandat 4446.389 kb on + strandat 4446.389 kb on + strandat 4446.389 kb on + strandat 4446.389 kb on + strandat 4446.389 kb on + strandat 4446.389 kb on + strandat 4446.390 kb on - strandat 4446.390 kb on - strandat 4446.865 kb on + strand, within PFLU_RS19640at 4446.865 kb on + strand, within PFLU_RS19640at 4446.865 kb on + strand, within PFLU_RS19640at 4446.866 kb on - strand, within PFLU_RS19640at 4446.866 kb on - strand, within PFLU_RS19640at 4446.866 kb on - strand, within PFLU_RS19640at 4446.874 kb on + strand, within PFLU_RS19640at 4446.874 kb on + strand, within PFLU_RS19640at 4446.874 kb on + strand, within PFLU_RS19640at 4446.874 kb on + strand, within PFLU_RS19640at 4446.874 kb on + strand, within PFLU_RS19640at 4446.875 kb on - strand, within PFLU_RS19640at 4447.130 kb on + strandat 4447.193 kb on + strand, within PFLU_RS19645at 4447.193 kb on + strand, within PFLU_RS19645at 4447.193 kb on + strand, within PFLU_RS19645at 4447.193 kb on + strand, within PFLU_RS19645at 4447.193 kb on + strand, within PFLU_RS19645at 4447.193 kb on + strand, within PFLU_RS19645at 4447.193 kb on + strand, within PFLU_RS19645at 4447.193 kb on + strand, within PFLU_RS19645at 4447.193 kb on + strand, within PFLU_RS19645at 4447.193 kb on + strand, within PFLU_RS19645at 4447.193 kb on + strand, within PFLU_RS19645at 4447.193 kb on + strand, within PFLU_RS19645at 4447.193 kb on + strand, within PFLU_RS19645at 4447.193 kb on + strand, within PFLU_RS19645at 4447.194 kb on - strand, within PFLU_RS19645at 4447.194 kb on - strand, within PFLU_RS19645at 4447.194 kb on - strand, within PFLU_RS19645at 4447.194 kb on - strand, within PFLU_RS19645at 4447.525 kb on + strandat 4447.526 kb on - strandat 4447.526 kb on - strandat 4447.572 kb on - strandat 4447.610 kb on + strandat 4447.610 kb on + strandat 4447.610 kb on + strandat 4447.610 kb on + strandat 4447.919 kb on + strand, within PFLU_RS19650at 4447.919 kb on + strand, within PFLU_RS19650at 4447.919 kb on + strand, within PFLU_RS19650at 4447.920 kb on - strand, within PFLU_RS19650at 4448.152 kb on + strand, within PFLU_RS19650at 4448.152 kb on + strand, within PFLU_RS19650at 4448.152 kb on + strand, within PFLU_RS19650at 4448.152 kb on + strand, within PFLU_RS19650at 4448.152 kb on + strand, within PFLU_RS19650at 4448.152 kb on + strand, within PFLU_RS19650at 4448.152 kb on + strand, within PFLU_RS19650at 4448.152 kb on + strand, within PFLU_RS19650at 4448.152 kb on + strand, within PFLU_RS19650at 4448.152 kb on + strand, within PFLU_RS19650at 4448.153 kb on - strand, within PFLU_RS19650at 4448.153 kb on - strand, within PFLU_RS19650at 4448.153 kb on - strand, within PFLU_RS19650at 4448.153 kb on - strand, within PFLU_RS19650at 4448.153 kb on - strand, within PFLU_RS19650at 4448.156 kb on + strand, within PFLU_RS19650at 4448.156 kb on + strand, within PFLU_RS19650at 4448.156 kb on + strand, within PFLU_RS19650at 4448.157 kb on - strand, within PFLU_RS19650at 4448.157 kb on - strand, within PFLU_RS19650at 4448.157 kb on - strand, within PFLU_RS19650at 4448.216 kb on + strand, within PFLU_RS19650at 4448.217 kb on - strand, within PFLU_RS19650at 4448.217 kb on - strand, within PFLU_RS19650at 4448.217 kb on - strand, within PFLU_RS19650at 4448.305 kb on + strand, within PFLU_RS19650at 4448.305 kb on + strand, within PFLU_RS19650at 4448.305 kb on + strand, within PFLU_RS19650at 4448.305 kb on + strand, within PFLU_RS19650at 4448.305 kb on + strand, within PFLU_RS19650at 4448.306 kb on - strand, within PFLU_RS19650at 4448.306 kb on - strand, within PFLU_RS19650at 4448.306 kb on - strand, within PFLU_RS19650at 4448.306 kb on - strand, within PFLU_RS19650at 4448.326 kb on + strand, within PFLU_RS19650at 4448.327 kb on - strand, within PFLU_RS19650at 4448.327 kb on - strand, within PFLU_RS19650

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Per-strain Table

Position Strand Gene LocusTag Fraction Growth at pH7 and (C) D-Glucose
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4,446,185 + PFLU_RS19635 0.90 +2.7
4,446,185 + PFLU_RS19635 0.90 -0.9
4,446,185 + PFLU_RS19635 0.90 -2.5
4,446,300 + +1.0
4,446,358 + -0.3
4,446,359 - +1.5
4,446,389 + +0.1
4,446,389 + +0.7
4,446,389 + +0.5
4,446,389 + -1.1
4,446,389 + +0.6
4,446,389 + +1.5
4,446,389 + +1.3
4,446,390 - +0.5
4,446,390 - -1.9
4,446,865 + PFLU_RS19640 0.66 +2.1
4,446,865 + PFLU_RS19640 0.66 -0.9
4,446,865 + PFLU_RS19640 0.66 -0.7
4,446,866 - PFLU_RS19640 0.66 -1.1
4,446,866 - PFLU_RS19640 0.66 -2.5
4,446,866 - PFLU_RS19640 0.66 -0.3
4,446,874 + PFLU_RS19640 0.67 +0.8
4,446,874 + PFLU_RS19640 0.67 +2.0
4,446,874 + PFLU_RS19640 0.67 -1.7
4,446,874 + PFLU_RS19640 0.67 -0.5
4,446,874 + PFLU_RS19640 0.67 +1.3
4,446,875 - PFLU_RS19640 0.67 -1.3
4,447,130 + +0.5
4,447,193 + PFLU_RS19645 0.17 -3.2
4,447,193 + PFLU_RS19645 0.17 +2.4
4,447,193 + PFLU_RS19645 0.17 +0.1
4,447,193 + PFLU_RS19645 0.17 -0.6
4,447,193 + PFLU_RS19645 0.17 +2.0
4,447,193 + PFLU_RS19645 0.17 -2.3
4,447,193 + PFLU_RS19645 0.17 -1.1
4,447,193 + PFLU_RS19645 0.17 -1.3
4,447,193 + PFLU_RS19645 0.17 -3.8
4,447,193 + PFLU_RS19645 0.17 +0.7
4,447,193 + PFLU_RS19645 0.17 +0.5
4,447,193 + PFLU_RS19645 0.17 +1.8
4,447,193 + PFLU_RS19645 0.17 -0.3
4,447,193 + PFLU_RS19645 0.17 -0.3
4,447,194 - PFLU_RS19645 0.17 -3.0
4,447,194 - PFLU_RS19645 0.17 -1.6
4,447,194 - PFLU_RS19645 0.17 -0.5
4,447,194 - PFLU_RS19645 0.17 -0.4
4,447,525 + +0.3
4,447,526 - +1.2
4,447,526 - -0.3
4,447,572 - -1.3
4,447,610 + -0.3
4,447,610 + -1.9
4,447,610 + -0.3
4,447,610 + -0.0
4,447,919 + PFLU_RS19650 0.41 +1.4
4,447,919 + PFLU_RS19650 0.41 +1.3
4,447,919 + PFLU_RS19650 0.41 -1.4
4,447,920 - PFLU_RS19650 0.41 -0.6
4,448,152 + PFLU_RS19650 0.65 +0.6
4,448,152 + PFLU_RS19650 0.65 -0.7
4,448,152 + PFLU_RS19650 0.65 +2.3
4,448,152 + PFLU_RS19650 0.65 -1.3
4,448,152 + PFLU_RS19650 0.65 -2.7
4,448,152 + PFLU_RS19650 0.65 -0.3
4,448,152 + PFLU_RS19650 0.65 -2.7
4,448,152 + PFLU_RS19650 0.65 +1.3
4,448,152 + PFLU_RS19650 0.65 +1.7
4,448,152 + PFLU_RS19650 0.65 +3.2
4,448,153 - PFLU_RS19650 0.65 +0.0
4,448,153 - PFLU_RS19650 0.65 +0.6
4,448,153 - PFLU_RS19650 0.65 -2.4
4,448,153 - PFLU_RS19650 0.65 +3.3
4,448,153 - PFLU_RS19650 0.65 -1.9
4,448,156 + PFLU_RS19650 0.66 -1.4
4,448,156 + PFLU_RS19650 0.66 +0.3
4,448,156 + PFLU_RS19650 0.66 -1.9
4,448,157 - PFLU_RS19650 0.66 -1.7
4,448,157 - PFLU_RS19650 0.66 -1.7
4,448,157 - PFLU_RS19650 0.66 -1.9
4,448,216 + PFLU_RS19650 0.72 +0.7
4,448,217 - PFLU_RS19650 0.72 +2.1
4,448,217 - PFLU_RS19650 0.72 +0.1
4,448,217 - PFLU_RS19650 0.72 +1.9
4,448,305 + PFLU_RS19650 0.81 -0.3
4,448,305 + PFLU_RS19650 0.81 +0.8
4,448,305 + PFLU_RS19650 0.81 +0.1
4,448,305 + PFLU_RS19650 0.81 +0.4
4,448,305 + PFLU_RS19650 0.81 +1.0
4,448,306 - PFLU_RS19650 0.81 +0.7
4,448,306 - PFLU_RS19650 0.81 -0.0
4,448,306 - PFLU_RS19650 0.81 -0.3
4,448,306 - PFLU_RS19650 0.81 +0.5
4,448,326 + PFLU_RS19650 0.83 +0.4
4,448,327 - PFLU_RS19650 0.83 +1.7
4,448,327 - PFLU_RS19650 0.83 -1.1

Or see this region's nucleotide sequence