Experiment: control, 1x iron with casaminos and vitamins, H2 media
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMJJ_RS04325 and MMJJ_RS04330 overlap by 20 nucleotides MMJJ_RS04330 and MMJJ_RS04335 are separated by 1 nucleotides MMJJ_RS04335 and MMJJ_RS04340 are separated by 12 nucleotides MMJJ_RS04340 and MMJJ_RS04345 are separated by 1 nucleotides MMJJ_RS04345 and MMJJ_RS04350 overlap by 8 nucleotides MMJJ_RS04350 and MMJJ_RS09405 are separated by 18 nucleotides
MMJJ_RS04325: MMJJ_RS04325 - tyrosine-type recombinase/integrase, at 807,960 to 808,928
_RS04325
MMJJ_RS04330: MMJJ_RS04330 - DUF2540 domain-containing protein, at 808,909 to 809,186
_RS04330
MMJJ_RS04335: MMJJ_RS04335 - minichromosome maintenance protein MCM, at 809,188 to 811,212
_RS04335
MMJJ_RS04340: MMJJ_RS04340 - hypothetical protein, at 811,225 to 811,440
_RS04340
MMJJ_RS04345: MMJJ_RS04345 - DUF2080 family transposase-associated protein, at 811,442 to 811,666
_RS04345
MMJJ_RS04350: MMJJ_RS04350 - hypothetical protein, at 811,659 to 812,162
_RS04350
MMJJ_RS09405: MMJJ_RS09405 - hypothetical protein, at 812,181 to 812,360
_RS09405
Position (kb) 809
810
811
812 Strain fitness (log2 ratio) -2
-1
0
1
2 at 808.200 kb on - strand, within MMJJ_RS04325 at 808.274 kb on + strand, within MMJJ_RS04325 at 808.281 kb on + strand, within MMJJ_RS04325 at 808.290 kb on - strand, within MMJJ_RS04325 at 808.347 kb on + strand, within MMJJ_RS04325 at 808.352 kb on - strand, within MMJJ_RS04325 at 808.374 kb on + strand, within MMJJ_RS04325 at 808.374 kb on + strand, within MMJJ_RS04325 at 808.450 kb on + strand, within MMJJ_RS04325 at 808.462 kb on + strand, within MMJJ_RS04325 at 808.462 kb on + strand, within MMJJ_RS04325 at 808.462 kb on + strand, within MMJJ_RS04325 at 808.490 kb on - strand, within MMJJ_RS04325 at 808.491 kb on + strand, within MMJJ_RS04325 at 808.500 kb on - strand, within MMJJ_RS04325 at 808.500 kb on - strand, within MMJJ_RS04325 at 808.500 kb on - strand, within MMJJ_RS04325 at 808.524 kb on - strand, within MMJJ_RS04325 at 808.525 kb on + strand, within MMJJ_RS04325 at 808.526 kb on - strand, within MMJJ_RS04325 at 808.527 kb on + strand, within MMJJ_RS04325 at 808.542 kb on - strand, within MMJJ_RS04325 at 808.620 kb on + strand, within MMJJ_RS04325 at 808.690 kb on - strand, within MMJJ_RS04325 at 808.704 kb on - strand, within MMJJ_RS04325 at 808.902 kb on - strand at 808.911 kb on + strand at 808.962 kb on - strand, within MMJJ_RS04330 at 808.962 kb on - strand, within MMJJ_RS04330 at 808.963 kb on + strand, within MMJJ_RS04330 at 808.966 kb on + strand, within MMJJ_RS04330 at 808.969 kb on - strand, within MMJJ_RS04330 at 808.969 kb on - strand at 808.970 kb on + strand, within MMJJ_RS04330 at 808.970 kb on + strand, within MMJJ_RS04330 at 808.970 kb on + strand, within MMJJ_RS04330 at 808.974 kb on - strand, within MMJJ_RS04330 at 809.094 kb on - strand, within MMJJ_RS04330 at 809.094 kb on - strand, within MMJJ_RS04330 at 809.094 kb on - strand, within MMJJ_RS04330 at 809.094 kb on - strand, within MMJJ_RS04330 at 809.094 kb on - strand, within MMJJ_RS04330 at 809.094 kb on - strand, within MMJJ_RS04330 at 809.094 kb on - strand, within MMJJ_RS04330 at 809.095 kb on + strand, within MMJJ_RS04330 at 809.095 kb on + strand, within MMJJ_RS04330 at 809.095 kb on + strand, within MMJJ_RS04330 at 809.095 kb on + strand, within MMJJ_RS04330 at 809.095 kb on + strand, within MMJJ_RS04330 at 809.095 kb on + strand, within MMJJ_RS04330 at 809.095 kb on + strand, within MMJJ_RS04330 at 809.095 kb on - strand, within MMJJ_RS04330 at 809.098 kb on - strand, within MMJJ_RS04330 at 809.098 kb on - strand, within MMJJ_RS04330 at 809.098 kb on - strand, within MMJJ_RS04330 at 809.098 kb on - strand, within MMJJ_RS04330 at 809.099 kb on + strand, within MMJJ_RS04330 at 809.099 kb on + strand, within MMJJ_RS04330 at 809.125 kb on - strand, within MMJJ_RS04330 at 809.126 kb on + strand, within MMJJ_RS04330 at 809.126 kb on + strand, within MMJJ_RS04330 at 809.126 kb on + strand, within MMJJ_RS04330 at 809.126 kb on + strand, within MMJJ_RS04330 at 809.126 kb on + strand, within MMJJ_RS04330 at 809.129 kb on + strand, within MMJJ_RS04330 at 809.159 kb on - strand at 809.160 kb on + strand at 809.167 kb on + strand at 809.167 kb on + strand at 809.261 kb on + strand at 809.267 kb on + strand at 809.287 kb on + strand at 809.330 kb on + strand at 809.362 kb on - strand at 809.362 kb on - strand at 809.364 kb on - strand at 809.367 kb on - strand at 809.373 kb on + strand at 809.384 kb on + strand at 809.468 kb on + strand, within MMJJ_RS04335 at 809.486 kb on - strand, within MMJJ_RS04335 at 809.486 kb on - strand, within MMJJ_RS04335 at 809.487 kb on + strand, within MMJJ_RS04335 at 809.487 kb on + strand, within MMJJ_RS04335 at 809.518 kb on - strand, within MMJJ_RS04335 at 809.608 kb on - strand, within MMJJ_RS04335 at 809.619 kb on + strand, within MMJJ_RS04335 at 809.632 kb on - strand, within MMJJ_RS04335 at 809.640 kb on - strand, within MMJJ_RS04335 at 809.679 kb on + strand, within MMJJ_RS04335 at 809.679 kb on + strand, within MMJJ_RS04335 at 809.688 kb on + strand, within MMJJ_RS04335 at 809.717 kb on + strand, within MMJJ_RS04335 at 809.774 kb on - strand, within MMJJ_RS04335 at 809.797 kb on + strand, within MMJJ_RS04335 at 809.873 kb on + strand, within MMJJ_RS04335 at 809.940 kb on - strand, within MMJJ_RS04335 at 810.004 kb on - strand, within MMJJ_RS04335 at 810.005 kb on + strand, within MMJJ_RS04335 at 810.005 kb on + strand, within MMJJ_RS04335 at 810.005 kb on + strand, within MMJJ_RS04335 at 810.033 kb on - strand, within MMJJ_RS04335 at 810.035 kb on - strand, within MMJJ_RS04335 at 810.035 kb on - strand, within MMJJ_RS04335 at 810.035 kb on - strand, within MMJJ_RS04335 at 810.036 kb on + strand, within MMJJ_RS04335 at 810.097 kb on - strand, within MMJJ_RS04335 at 810.098 kb on + strand, within MMJJ_RS04335 at 810.103 kb on - strand, within MMJJ_RS04335 at 810.104 kb on + strand, within MMJJ_RS04335 at 810.115 kb on - strand, within MMJJ_RS04335 at 810.115 kb on - strand, within MMJJ_RS04335 at 810.115 kb on - strand, within MMJJ_RS04335 at 810.116 kb on + strand, within MMJJ_RS04335 at 810.126 kb on - strand, within MMJJ_RS04335 at 810.203 kb on - strand, within MMJJ_RS04335 at 810.457 kb on - strand, within MMJJ_RS04335 at 810.457 kb on - strand, within MMJJ_RS04335 at 810.457 kb on - strand, within MMJJ_RS04335 at 810.458 kb on + strand, within MMJJ_RS04335 at 810.604 kb on - strand, within MMJJ_RS04335 at 810.604 kb on - strand, within MMJJ_RS04335 at 810.682 kb on - strand, within MMJJ_RS04335 at 810.682 kb on - strand, within MMJJ_RS04335 at 810.682 kb on - strand, within MMJJ_RS04335 at 810.682 kb on - strand, within MMJJ_RS04335 at 810.683 kb on + strand, within MMJJ_RS04335 at 810.683 kb on + strand, within MMJJ_RS04335 at 810.683 kb on + strand, within MMJJ_RS04335 at 810.683 kb on + strand, within MMJJ_RS04335 at 810.686 kb on - strand, within MMJJ_RS04335 at 810.686 kb on - strand, within MMJJ_RS04335 at 810.687 kb on + strand, within MMJJ_RS04335 at 810.714 kb on - strand, within MMJJ_RS04335 at 810.715 kb on + strand, within MMJJ_RS04335 at 810.810 kb on + strand, within MMJJ_RS04335 at 810.810 kb on + strand, within MMJJ_RS04335 at 810.856 kb on - strand, within MMJJ_RS04335 at 810.856 kb on - strand, within MMJJ_RS04335 at 810.857 kb on + strand, within MMJJ_RS04335 at 811.016 kb on - strand at 811.065 kb on + strand at 811.136 kb on + strand at 811.186 kb on - strand at 811.276 kb on + strand, within MMJJ_RS04340 at 811.300 kb on - strand, within MMJJ_RS04340 at 811.300 kb on - strand, within MMJJ_RS04340 at 811.301 kb on + strand, within MMJJ_RS04340 at 811.321 kb on + strand, within MMJJ_RS04340 at 811.333 kb on + strand, within MMJJ_RS04340 at 811.407 kb on - strand, within MMJJ_RS04340 at 811.571 kb on - strand, within MMJJ_RS04345 at 811.582 kb on - strand, within MMJJ_RS04345 at 811.582 kb on - strand, within MMJJ_RS04345 at 811.583 kb on + strand, within MMJJ_RS04345 at 811.583 kb on + strand, within MMJJ_RS04345 at 811.635 kb on + strand, within MMJJ_RS04345 at 811.655 kb on - strand at 811.656 kb on + strand at 811.788 kb on - strand, within MMJJ_RS04350 at 811.789 kb on + strand, within MMJJ_RS04350 at 811.789 kb on + strand, within MMJJ_RS04350 at 811.932 kb on + strand, within MMJJ_RS04350 at 811.932 kb on + strand, within MMJJ_RS04350 at 811.965 kb on - strand, within MMJJ_RS04350 at 811.966 kb on + strand, within MMJJ_RS04350 at 811.966 kb on + strand, within MMJJ_RS04350 at 812.059 kb on - strand, within MMJJ_RS04350 at 812.076 kb on - strand, within MMJJ_RS04350 at 812.076 kb on - strand, within MMJJ_RS04350 at 812.089 kb on + strand, within MMJJ_RS04350 at 812.124 kb on + strand at 812.127 kb on - strand at 812.127 kb on - strand at 812.128 kb on + strand at 812.163 kb on + strand at 812.180 kb on + strand at 812.183 kb on + strand at 812.203 kb on + strand, within MMJJ_RS09405
Per-strain Table
Position Strand Gene LocusTag Fraction control, 1x iron with casaminos and vitamins, H2 media remove 808,200 - MMJJ_RS04325 0.25 +0.6 808,274 + MMJJ_RS04325 0.32 -1.2 808,281 + MMJJ_RS04325 0.33 +0.4 808,290 - MMJJ_RS04325 0.34 +0.6 808,347 + MMJJ_RS04325 0.40 -0.6 808,352 - MMJJ_RS04325 0.40 -0.5 808,374 + MMJJ_RS04325 0.43 -1.4 808,374 + MMJJ_RS04325 0.43 -0.1 808,450 + MMJJ_RS04325 0.51 -0.8 808,462 + MMJJ_RS04325 0.52 +0.1 808,462 + MMJJ_RS04325 0.52 +0.7 808,462 + MMJJ_RS04325 0.52 -0.7 808,490 - MMJJ_RS04325 0.55 +0.7 808,491 + MMJJ_RS04325 0.55 -0.4 808,500 - MMJJ_RS04325 0.56 -0.7 808,500 - MMJJ_RS04325 0.56 +0.1 808,500 - MMJJ_RS04325 0.56 -0.1 808,524 - MMJJ_RS04325 0.58 -1.3 808,525 + MMJJ_RS04325 0.58 -0.2 808,526 - MMJJ_RS04325 0.58 +0.3 808,527 + MMJJ_RS04325 0.59 +0.0 808,542 - MMJJ_RS04325 0.60 +1.0 808,620 + MMJJ_RS04325 0.68 +0.5 808,690 - MMJJ_RS04325 0.75 -2.3 808,704 - MMJJ_RS04325 0.77 +0.6 808,902 - +0.3 808,911 + -0.5 808,962 - MMJJ_RS04330 0.19 +0.6 808,962 - MMJJ_RS04330 0.19 +0.1 808,963 + MMJJ_RS04330 0.19 +0.5 808,966 + MMJJ_RS04330 0.21 +0.5 808,969 - MMJJ_RS04330 0.22 -1.0 808,969 - -1.2 808,970 + MMJJ_RS04330 0.22 -0.1 808,970 + MMJJ_RS04330 0.22 +0.9 808,970 + MMJJ_RS04330 0.22 +0.7 808,974 - MMJJ_RS04330 0.23 -0.5 809,094 - MMJJ_RS04330 0.67 -1.3 809,094 - MMJJ_RS04330 0.67 +1.0 809,094 - MMJJ_RS04330 0.67 +0.1 809,094 - MMJJ_RS04330 0.67 +0.3 809,094 - MMJJ_RS04330 0.67 +0.1 809,094 - MMJJ_RS04330 0.67 -2.0 809,094 - MMJJ_RS04330 0.67 -1.5 809,095 + MMJJ_RS04330 0.67 +1.1 809,095 + MMJJ_RS04330 0.67 -0.8 809,095 + MMJJ_RS04330 0.67 +0.4 809,095 + MMJJ_RS04330 0.67 +1.6 809,095 + MMJJ_RS04330 0.67 -0.1 809,095 + MMJJ_RS04330 0.67 -0.0 809,095 + MMJJ_RS04330 0.67 +0.7 809,095 - MMJJ_RS04330 0.67 -0.3 809,098 - MMJJ_RS04330 0.68 +1.1 809,098 - MMJJ_RS04330 0.68 -1.4 809,098 - MMJJ_RS04330 0.68 -0.2 809,098 - MMJJ_RS04330 0.68 -1.8 809,099 + MMJJ_RS04330 0.68 -1.1 809,099 + MMJJ_RS04330 0.68 -0.4 809,125 - MMJJ_RS04330 0.78 -0.3 809,126 + MMJJ_RS04330 0.78 +0.4 809,126 + MMJJ_RS04330 0.78 +1.0 809,126 + MMJJ_RS04330 0.78 +0.1 809,126 + MMJJ_RS04330 0.78 -0.5 809,126 + MMJJ_RS04330 0.78 -0.6 809,129 + MMJJ_RS04330 0.79 +0.5 809,159 - -0.5 809,160 + -2.0 809,167 + -1.3 809,167 + +1.2 809,261 + +1.2 809,267 + -2.0 809,287 + +0.6 809,330 + +0.9 809,362 - -0.4 809,362 - -0.5 809,364 - -1.2 809,367 - +1.0 809,373 + +0.2 809,384 + -0.4 809,468 + MMJJ_RS04335 0.14 +0.4 809,486 - MMJJ_RS04335 0.15 +0.7 809,486 - MMJJ_RS04335 0.15 -0.4 809,487 + MMJJ_RS04335 0.15 +0.3 809,487 + MMJJ_RS04335 0.15 -0.1 809,518 - MMJJ_RS04335 0.16 -0.9 809,608 - MMJJ_RS04335 0.21 +0.1 809,619 + MMJJ_RS04335 0.21 -0.5 809,632 - MMJJ_RS04335 0.22 +0.7 809,640 - MMJJ_RS04335 0.22 -1.1 809,679 + MMJJ_RS04335 0.24 -0.3 809,679 + MMJJ_RS04335 0.24 +1.2 809,688 + MMJJ_RS04335 0.25 +0.5 809,717 + MMJJ_RS04335 0.26 +0.3 809,774 - MMJJ_RS04335 0.29 +0.2 809,797 + MMJJ_RS04335 0.30 -0.5 809,873 + MMJJ_RS04335 0.34 +0.9 809,940 - MMJJ_RS04335 0.37 +0.8 810,004 - MMJJ_RS04335 0.40 +1.0 810,005 + MMJJ_RS04335 0.40 +0.0 810,005 + MMJJ_RS04335 0.40 -1.2 810,005 + MMJJ_RS04335 0.40 +0.2 810,033 - MMJJ_RS04335 0.42 +0.4 810,035 - MMJJ_RS04335 0.42 -0.0 810,035 - MMJJ_RS04335 0.42 +1.8 810,035 - MMJJ_RS04335 0.42 -2.2 810,036 + MMJJ_RS04335 0.42 -1.2 810,097 - MMJJ_RS04335 0.45 +0.4 810,098 + MMJJ_RS04335 0.45 -1.6 810,103 - MMJJ_RS04335 0.45 +0.8 810,104 + MMJJ_RS04335 0.45 -1.0 810,115 - MMJJ_RS04335 0.46 -1.3 810,115 - MMJJ_RS04335 0.46 -0.1 810,115 - MMJJ_RS04335 0.46 +0.9 810,116 + MMJJ_RS04335 0.46 -1.3 810,126 - MMJJ_RS04335 0.46 +0.5 810,203 - MMJJ_RS04335 0.50 +1.2 810,457 - MMJJ_RS04335 0.63 +0.9 810,457 - MMJJ_RS04335 0.63 +0.7 810,457 - MMJJ_RS04335 0.63 +1.3 810,458 + MMJJ_RS04335 0.63 +1.3 810,604 - MMJJ_RS04335 0.70 +0.3 810,604 - MMJJ_RS04335 0.70 +1.3 810,682 - MMJJ_RS04335 0.74 -0.0 810,682 - MMJJ_RS04335 0.74 -1.8 810,682 - MMJJ_RS04335 0.74 +0.5 810,682 - MMJJ_RS04335 0.74 +1.2 810,683 + MMJJ_RS04335 0.74 -0.2 810,683 + MMJJ_RS04335 0.74 +0.4 810,683 + MMJJ_RS04335 0.74 -1.4 810,683 + MMJJ_RS04335 0.74 -0.4 810,686 - MMJJ_RS04335 0.74 +0.4 810,686 - MMJJ_RS04335 0.74 +0.8 810,687 + MMJJ_RS04335 0.74 +1.1 810,714 - MMJJ_RS04335 0.75 -1.9 810,715 + MMJJ_RS04335 0.75 +0.9 810,810 + MMJJ_RS04335 0.80 +0.2 810,810 + MMJJ_RS04335 0.80 +0.6 810,856 - MMJJ_RS04335 0.82 -0.0 810,856 - MMJJ_RS04335 0.82 +0.8 810,857 + MMJJ_RS04335 0.82 -0.7 811,016 - -1.2 811,065 + -0.6 811,136 + +1.0 811,186 - +1.5 811,276 + MMJJ_RS04340 0.24 +0.3 811,300 - MMJJ_RS04340 0.35 +0.5 811,300 - MMJJ_RS04340 0.35 +0.8 811,301 + MMJJ_RS04340 0.35 +0.0 811,321 + MMJJ_RS04340 0.44 -0.9 811,333 + MMJJ_RS04340 0.50 +0.3 811,407 - MMJJ_RS04340 0.84 +0.3 811,571 - MMJJ_RS04345 0.57 +0.2 811,582 - MMJJ_RS04345 0.62 +0.1 811,582 - MMJJ_RS04345 0.62 +1.0 811,583 + MMJJ_RS04345 0.63 -1.1 811,583 + MMJJ_RS04345 0.63 -1.4 811,635 + MMJJ_RS04345 0.86 -0.6 811,655 - +0.1 811,656 + +0.9 811,788 - MMJJ_RS04350 0.26 +2.2 811,789 + MMJJ_RS04350 0.26 -0.3 811,789 + MMJJ_RS04350 0.26 -0.1 811,932 + MMJJ_RS04350 0.54 +1.7 811,932 + MMJJ_RS04350 0.54 +0.1 811,965 - MMJJ_RS04350 0.61 +0.2 811,966 + MMJJ_RS04350 0.61 -0.7 811,966 + MMJJ_RS04350 0.61 -0.2 812,059 - MMJJ_RS04350 0.79 +1.3 812,076 - MMJJ_RS04350 0.83 +2.2 812,076 - MMJJ_RS04350 0.83 -0.3 812,089 + MMJJ_RS04350 0.85 +0.4 812,124 + -0.9 812,127 - +0.5 812,127 - -0.0 812,128 + -0.1 812,163 + -0.4 812,180 + -0.3 812,183 + +0.4 812,203 + MMJJ_RS09405 0.12 -0.6
Or see this region's nucleotide sequence