Experiment: L-Histidine
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS18630 and BPHYT_RS18635 are separated by 103 nucleotides BPHYT_RS18635 and BPHYT_RS18640 are separated by 264 nucleotides
BPHYT_RS18630: BPHYT_RS18630 - allophanate hydrolase, at 4,189,732 to 4,190,784
_RS18630
BPHYT_RS18635: BPHYT_RS18635 - LamB/YcsF family protein, at 4,190,888 to 4,191,640
_RS18635
BPHYT_RS18640: BPHYT_RS18640 - membrane protein, at 4,191,905 to 4,192,654
_RS18640
Position (kb)
4190
4191
4192 Strain fitness (log2 ratio)
-1
0
1
2 at 4190.066 kb on - strand, within BPHYT_RS18630 at 4190.475 kb on + strand, within BPHYT_RS18630 at 4190.703 kb on + strand at 4190.704 kb on - strand at 4190.704 kb on - strand at 4190.707 kb on + strand at 4190.708 kb on - strand at 4190.708 kb on - strand at 4190.782 kb on + strand at 4190.783 kb on - strand at 4191.122 kb on + strand, within BPHYT_RS18635 at 4191.137 kb on + strand, within BPHYT_RS18635 at 4191.138 kb on - strand, within BPHYT_RS18635 at 4191.774 kb on - strand at 4191.774 kb on - strand at 4191.774 kb on - strand at 4191.774 kb on - strand at 4191.785 kb on + strand at 4191.925 kb on - strand at 4191.925 kb on - strand at 4191.925 kb on - strand at 4191.969 kb on + strand at 4191.969 kb on + strand at 4192.088 kb on - strand, within BPHYT_RS18640 at 4192.214 kb on + strand, within BPHYT_RS18640 at 4192.215 kb on - strand, within BPHYT_RS18640 at 4192.215 kb on - strand, within BPHYT_RS18640 at 4192.215 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Histidine remove 4,190,066 - BPHYT_RS18630 0.32 -0.4 4,190,475 + BPHYT_RS18630 0.71 -0.0 4,190,703 + -0.2 4,190,704 - +0.9 4,190,704 - -0.2 4,190,707 + -0.3 4,190,708 - -0.3 4,190,708 - +0.6 4,190,782 + +0.7 4,190,783 - +0.4 4,191,122 + BPHYT_RS18635 0.31 -1.0 4,191,137 + BPHYT_RS18635 0.33 -1.2 4,191,138 - BPHYT_RS18635 0.33 +0.2 4,191,774 - +0.3 4,191,774 - -0.1 4,191,774 - +0.3 4,191,774 - -0.3 4,191,785 + +0.4 4,191,925 - +2.0 4,191,925 - -0.0 4,191,925 - -0.5 4,191,969 + +0.2 4,191,969 + -0.4 4,192,088 - BPHYT_RS18640 0.24 +0.1 4,192,214 + BPHYT_RS18640 0.41 -1.0 4,192,215 - BPHYT_RS18640 0.41 +0.2 4,192,215 - BPHYT_RS18640 0.41 +0.8 4,192,215 - -0.6
Or see this region's nucleotide sequence