Experiment: L-Histidine
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS08405 and BPHYT_RS08410 are separated by 156 nucleotides BPHYT_RS08410 and BPHYT_RS08415 are separated by 30 nucleotides BPHYT_RS08415 and BPHYT_RS08420 are separated by 136 nucleotides
BPHYT_RS08405: BPHYT_RS08405 - hypothetical protein, at 1,927,201 to 1,927,551
_RS08405
BPHYT_RS08410: BPHYT_RS08410 - dehydrogenase, at 1,927,708 to 1,928,649
_RS08410
BPHYT_RS08415: BPHYT_RS08415 - hypothetical protein, at 1,928,680 to 1,929,063
_RS08415
BPHYT_RS08420: BPHYT_RS08420 - histidine kinase, at 1,929,200 to 1,929,637
_RS08420
Position (kb) 1927
1928
1929 Strain fitness (log2 ratio) -2
-1
0
1 at 1926.752 kb on - strand at 1927.046 kb on + strand at 1927.148 kb on + strand at 1927.149 kb on - strand at 1927.488 kb on + strand, within BPHYT_RS08405 at 1928.065 kb on - strand, within BPHYT_RS08410 at 1928.593 kb on + strand at 1928.628 kb on + strand at 1928.898 kb on + strand, within BPHYT_RS08415 at 1928.929 kb on + strand, within BPHYT_RS08415 at 1928.948 kb on - strand, within BPHYT_RS08415 at 1929.294 kb on - strand, within BPHYT_RS08420
Per-strain Table
Position Strand Gene LocusTag Fraction L-Histidine remove 1,926,752 - -0.5 1,927,046 + +1.5 1,927,148 + -2.1 1,927,149 - -0.9 1,927,488 + BPHYT_RS08405 0.82 +0.1 1,928,065 - BPHYT_RS08410 0.38 -1.2 1,928,593 + +1.5 1,928,628 + +0.2 1,928,898 + BPHYT_RS08415 0.57 -2.1 1,928,929 + BPHYT_RS08415 0.65 -2.3 1,928,948 - BPHYT_RS08415 0.70 -0.1 1,929,294 - BPHYT_RS08420 0.21 +0.1
Or see this region's nucleotide sequence