Experiment: MoYLS4 with Rifampicin 0.2 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt valS and DvMF_2766 are separated by 383 nucleotides DvMF_2766 and DvMF_2767 are separated by 93 nucleotides DvMF_2767 and DvMF_2768 are separated by 269 nucleotides DvMF_2768 and DvMF_2769 are separated by 69 nucleotides
DvMF_2765: valS - valyl-tRNA synthetase (RefSeq), at 3,497,253 to 3,499,904
valS
DvMF_2766: DvMF_2766 - hypothetical protein (RefSeq), at 3,500,288 to 3,500,746
_2766
DvMF_2767: DvMF_2767 - uroporphyrin-III C-methyltransferase (RefSeq), at 3,500,840 to 3,502,351
_2767
DvMF_2768: DvMF_2768 - MOSC domain containing protein (RefSeq), at 3,502,621 to 3,503,055
_2768
DvMF_2769: DvMF_2769 - phosphoribosylglycinamide formyltransferase (RefSeq), at 3,503,125 to 3,503,808
_2769
Position (kb)
3500
3501
3502
3503 Strain fitness (log2 ratio)
-2
-1
0
1 at 3500.333 kb on + strand at 3500.426 kb on - strand, within DvMF_2766 at 3500.564 kb on + strand, within DvMF_2766 at 3500.683 kb on + strand, within DvMF_2766 at 3500.686 kb on - strand, within DvMF_2766 at 3502.471 kb on + strand at 3502.497 kb on + strand at 3502.623 kb on - strand at 3503.120 kb on + strand at 3503.327 kb on + strand, within DvMF_2769
Per-strain Table
Position Strand Gene LocusTag Fraction MoYLS4 with Rifampicin 0.2 mg/ml remove 3,500,333 + -1.0 3,500,426 - DvMF_2766 0.30 -0.0 3,500,564 + DvMF_2766 0.60 -0.5 3,500,683 + DvMF_2766 0.86 -0.7 3,500,686 - DvMF_2766 0.87 -0.8 3,502,471 + -0.1 3,502,497 + -1.2 3,502,623 - -0.7 3,503,120 + -1.1 3,503,327 + DvMF_2769 0.30 -2.2
Or see this region's nucleotide sequence