Experiment: MoYLS4 with Rifampicin 0.2 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt DvMF_1004 and DvMF_1005 are separated by 71 nucleotides DvMF_1005 and DvMF_1006 are separated by 41 nucleotides DvMF_1006 and DvMF_1007 overlap by 4 nucleotides DvMF_1007 and DvMF_1008 are separated by 26 nucleotides
DvMF_1004: DvMF_1004 - short-chain dehydrogenase/reductase SDR (RefSeq), at 1,219,486 to 1,220,316
_1004
DvMF_1005: DvMF_1005 - acyl carrier protein, putative (RefSeq), at 1,220,388 to 1,220,801
_1005
DvMF_1006: DvMF_1006 - Beta-ketoacyl synthase (RefSeq), at 1,220,843 to 1,222,219
_1006
DvMF_1007: DvMF_1007 - hypothetical protein (RefSeq), at 1,222,216 to 1,222,743
_1007
DvMF_1008: DvMF_1008 - aminotransferase class I and II (RefSeq), at 1,222,770 to 1,224,110
_1008
Position (kb)
1220
1221
1222
1223 Strain fitness (log2 ratio)
-2
-1
0
1 at 1220.050 kb on + strand, within DvMF_1004 at 1220.058 kb on - strand, within DvMF_1004 at 1220.058 kb on - strand, within DvMF_1004 at 1220.264 kb on + strand at 1220.282 kb on - strand at 1220.399 kb on + strand at 1220.437 kb on - strand, within DvMF_1005 at 1220.927 kb on + strand at 1221.083 kb on - strand, within DvMF_1006 at 1221.229 kb on + strand, within DvMF_1006 at 1221.309 kb on + strand, within DvMF_1006 at 1221.409 kb on + strand, within DvMF_1006 at 1221.412 kb on - strand, within DvMF_1006 at 1221.456 kb on - strand, within DvMF_1006 at 1221.582 kb on + strand, within DvMF_1006 at 1221.985 kb on + strand, within DvMF_1006 at 1222.042 kb on + strand, within DvMF_1006 at 1222.113 kb on - strand at 1222.493 kb on - strand, within DvMF_1007 at 1222.626 kb on + strand, within DvMF_1007 at 1222.867 kb on + strand at 1223.206 kb on - strand, within DvMF_1008
Per-strain Table
Position Strand Gene LocusTag Fraction MoYLS4 with Rifampicin 0.2 mg/ml remove 1,220,050 + DvMF_1004 0.68 +0.3 1,220,058 - DvMF_1004 0.69 +0.4 1,220,058 - DvMF_1004 0.69 -0.2 1,220,264 + -1.1 1,220,282 - -2.5 1,220,399 + -0.6 1,220,437 - DvMF_1005 0.12 -1.6 1,220,927 + -2.3 1,221,083 - DvMF_1006 0.17 -1.0 1,221,229 + DvMF_1006 0.28 -1.0 1,221,309 + DvMF_1006 0.34 -0.1 1,221,409 + DvMF_1006 0.41 -0.2 1,221,412 - DvMF_1006 0.41 -0.1 1,221,456 - DvMF_1006 0.45 -0.3 1,221,582 + DvMF_1006 0.54 +0.8 1,221,985 + DvMF_1006 0.83 -0.1 1,222,042 + DvMF_1006 0.87 -1.1 1,222,113 - +0.2 1,222,493 - DvMF_1007 0.52 -0.9 1,222,626 + DvMF_1007 0.78 -1.3 1,222,867 + -0.2 1,223,206 - DvMF_1008 0.33 -1.4
Or see this region's nucleotide sequence