Experiment: 5-Hydroxymethylfurfural 10 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_1935 and Shew_1936 are separated by 164 nucleotides Shew_1936 and Shew_1937 are separated by 125 nucleotides Shew_1937 and Shew_1938 are separated by 9 nucleotides Shew_1938 and Shew_1939 overlap by 4 nucleotides Shew_1939 and ansA are separated by 59 nucleotides
Shew_1935: Shew_1935 - hypothetical protein (RefSeq), at 2,235,127 to 2,236,905
_1935
Shew_1936: Shew_1936 - two component transcriptional regulator (RefSeq), at 2,237,070 to 2,237,750
_1936
Shew_1937: Shew_1937 - peptidase M14, carboxypeptidase A (RefSeq), at 2,237,876 to 2,239,003
_1937
Shew_1938: Shew_1938 - hypothetical protein (RefSeq), at 2,239,013 to 2,239,330
_1938
Shew_1939: Shew_1939 - hypothetical protein (RefSeq), at 2,239,327 to 2,239,608
_1939
Shew_1940: ansA - cytoplasmic asparaginase I (RefSeq), at 2,239,668 to 2,240,681
ansA
Position (kb)
2237
2238
2239
2240 Strain fitness (log2 ratio)
-1
0
1
2 at 2238.296 kb on - strand, within Shew_1937 at 2238.312 kb on + strand, within Shew_1937 at 2238.357 kb on - strand, within Shew_1937 at 2238.804 kb on + strand, within Shew_1937 at 2239.547 kb on - strand, within Shew_1939 at 2239.726 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction 5-Hydroxymethylfurfural 10 mM remove 2,238,296 - Shew_1937 0.37 +1.4 2,238,312 + Shew_1937 0.39 +1.2 2,238,357 - Shew_1937 0.43 +0.6 2,238,804 + Shew_1937 0.82 +0.5 2,239,547 - Shew_1939 0.78 +0.0 2,239,726 + +1.9
Or see this region's nucleotide sequence