Experiment: 5-Hydroxymethylfurfural 10 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_0065 and Shew_0066 are separated by 274 nucleotides Shew_0066 and Shew_0067 are separated by 1 nucleotides Shew_0067 and Shew_0068 are separated by 214 nucleotides
Shew_0065: Shew_0065 - formate dehydrogenase, gamma subunit (RefSeq), at 78,618 to 79,610
_0065
Shew_0066: Shew_0066 - hypothetical protein (RefSeq), at 79,885 to 80,250
_0066
Shew_0067: Shew_0067 - hypothetical protein (RefSeq), at 80,252 to 80,761
_0067
Shew_0068: Shew_0068 - fumarate lyase (RefSeq), at 80,976 to 82,355
_0068
Position (kb) 80
81 Strain fitness (log2 ratio) -2
-1
0
1 at 79.259 kb on + strand, within Shew_0065 at 79.267 kb on - strand, within Shew_0065 at 79.414 kb on + strand, within Shew_0065 at 79.538 kb on - strand at 79.717 kb on - strand at 79.744 kb on + strand at 79.758 kb on - strand at 80.040 kb on + strand, within Shew_0066 at 80.040 kb on + strand, within Shew_0066 at 80.048 kb on - strand, within Shew_0066 at 80.110 kb on + strand, within Shew_0066 at 80.118 kb on - strand, within Shew_0066 at 80.118 kb on - strand, within Shew_0066 at 80.209 kb on - strand, within Shew_0066 at 80.806 kb on + strand at 80.914 kb on - strand at 81.325 kb on + strand, within Shew_0068 at 81.361 kb on + strand, within Shew_0068 at 81.361 kb on + strand, within Shew_0068 at 81.399 kb on + strand, within Shew_0068 at 81.399 kb on + strand, within Shew_0068 at 81.407 kb on - strand, within Shew_0068 at 81.690 kb on - strand, within Shew_0068
Per-strain Table
Position Strand Gene LocusTag Fraction 5-Hydroxymethylfurfural 10 mM remove 79,259 + Shew_0065 0.65 +0.4 79,267 - Shew_0065 0.65 -0.3 79,414 + Shew_0065 0.80 +0.6 79,538 - -1.2 79,717 - -2.1 79,744 + -0.4 79,758 - +0.2 80,040 + Shew_0066 0.42 -0.5 80,040 + Shew_0066 0.42 +0.3 80,048 - Shew_0066 0.45 -2.2 80,110 + Shew_0066 0.61 -0.8 80,118 - Shew_0066 0.64 -1.2 80,118 - Shew_0066 0.64 +0.4 80,209 - Shew_0066 0.89 +0.1 80,806 + -0.3 80,914 - +0.6 81,325 + Shew_0068 0.25 -0.1 81,361 + Shew_0068 0.28 -0.3 81,361 + Shew_0068 0.28 -0.0 81,399 + Shew_0068 0.31 -0.1 81,399 + Shew_0068 0.31 +0.1 81,407 - Shew_0068 0.31 -0.1 81,690 - Shew_0068 0.52 -0.0
Or see this region's nucleotide sequence