Strain Fitness in Ralstonia solanacearum GMI1000 around RS_RS01700

Experiment: M63_quarter_strength

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntRS_RS01695 and RS_RS01700 are separated by 28 nucleotidesRS_RS01700 and RS_RS01705 are separated by 110 nucleotidesRS_RS01705 and RS_RS01710 overlap by 32 nucleotidesRS_RS01710 and RS_RS01715 are separated by 46 nucleotides RS_RS01695: RS_RS01695 - glutamate--cysteine ligase, at 376,641 to 377,936 _RS01695 RS_RS01700: RS_RS01700 - glutathione synthetase, at 377,965 to 378,939 _RS01700 RS_RS01705: RS_RS01705 - PTS fructose transporter subunit IIA, at 379,050 to 379,505 _RS01705 RS_RS01710: RS_RS01710 - HPr family phosphocarrier protein, at 379,474 to 379,743 _RS01710 RS_RS01715: RS_RS01715 - phosphoenolpyruvate--protein phosphotransferase, at 379,790 to 381,550 _RS01715 Position (kb) 377 378 379Strain fitness (log2 ratio) -3 -2 -1 0 1at 377.512 kb on - strand, within RS_RS01695at 377.946 kb on + strandat 377.947 kb on - strandat 378.010 kb on + strandat 378.067 kb on + strand, within RS_RS01700at 378.068 kb on - strand, within RS_RS01700at 378.068 kb on - strand, within RS_RS01700at 378.068 kb on - strand, within RS_RS01700at 378.244 kb on + strand, within RS_RS01700at 378.245 kb on - strand, within RS_RS01700at 378.245 kb on - strand, within RS_RS01700at 378.436 kb on + strand, within RS_RS01700at 378.559 kb on + strand, within RS_RS01700at 378.559 kb on + strand, within RS_RS01700at 378.560 kb on - strand, within RS_RS01700at 379.017 kb on - strandat 379.017 kb on - strandat 379.357 kb on - strand, within RS_RS01705at 379.357 kb on - strand, within RS_RS01705at 379.473 kb on + strandat 379.473 kb on + strandat 379.473 kb on + strandat 379.473 kb on + strandat 379.474 kb on - strandat 379.504 kb on - strandat 379.504 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction M63_quarter_strength
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377,512 - RS_RS01695 0.67 +0.5
377,946 + -0.1
377,947 - -0.2
378,010 + +0.1
378,067 + RS_RS01700 0.10 -1.8
378,068 - RS_RS01700 0.11 -2.8
378,068 - RS_RS01700 0.11 +0.5
378,068 - RS_RS01700 0.11 +0.2
378,244 + RS_RS01700 0.29 -1.1
378,245 - RS_RS01700 0.29 -0.3
378,245 - RS_RS01700 0.29 -0.8
378,436 + RS_RS01700 0.48 -0.4
378,559 + RS_RS01700 0.61 -0.3
378,559 + RS_RS01700 0.61 -1.2
378,560 - RS_RS01700 0.61 +0.5
379,017 - +0.0
379,017 - -1.1
379,357 - RS_RS01705 0.67 -2.3
379,357 - RS_RS01705 0.67 -2.6
379,473 + -0.5
379,473 + +0.1
379,473 + -1.2
379,473 + +0.1
379,474 - -1.1
379,504 - -1.7
379,504 - -0.9

Or see this region's nucleotide sequence