Experiment: R2A with Ferric chloride 1.5625 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt LRK54_RS05490 and LRK54_RS05495 are separated by 694 nucleotides LRK54_RS05495 and LRK54_RS05500 are separated by 53 nucleotides LRK54_RS05500 and LRK54_RS05505 are separated by 137 nucleotides
LRK54_RS05490: LRK54_RS05490 - energy transducer TonB, at 1,185,557 to 1,186,585
_RS05490
LRK54_RS05495: LRK54_RS05495 - met regulon transcriptional regulator MetJ, at 1,187,280 to 1,187,507
_RS05495
LRK54_RS05500: LRK54_RS05500 - hypothetical protein, at 1,187,561 to 1,187,980
_RS05500
LRK54_RS05505: LRK54_RS05505 - glycine cleavage system protein GcvH, at 1,188,118 to 1,188,513
_RS05505
Position (kb)
1187
1188 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 1186.305 kb on + strand, within LRK54_RS05490 at 1186.305 kb on + strand, within LRK54_RS05490 at 1186.306 kb on - strand, within LRK54_RS05490 at 1186.306 kb on - strand, within LRK54_RS05490 at 1186.306 kb on - strand, within LRK54_RS05490 at 1186.561 kb on + strand at 1186.561 kb on + strand at 1186.561 kb on + strand at 1186.561 kb on + strand at 1186.562 kb on - strand at 1186.568 kb on + strand at 1186.568 kb on + strand at 1186.568 kb on + strand at 1186.568 kb on + strand at 1186.568 kb on + strand at 1186.568 kb on + strand at 1186.568 kb on + strand at 1186.568 kb on + strand at 1186.568 kb on + strand at 1186.568 kb on + strand at 1186.568 kb on + strand at 1186.568 kb on + strand at 1186.568 kb on + strand at 1186.568 kb on + strand at 1186.568 kb on + strand at 1186.568 kb on + strand at 1186.568 kb on + strand at 1186.568 kb on + strand at 1186.568 kb on + strand at 1186.568 kb on + strand at 1186.568 kb on + strand at 1186.568 kb on + strand at 1186.568 kb on + strand at 1186.568 kb on + strand at 1186.568 kb on + strand at 1186.568 kb on + strand at 1186.568 kb on + strand at 1186.568 kb on + strand at 1186.568 kb on + strand at 1186.568 kb on + strand at 1186.568 kb on + strand at 1186.568 kb on + strand at 1186.568 kb on + strand at 1186.568 kb on + strand at 1186.568 kb on + strand at 1186.568 kb on + strand at 1186.568 kb on + strand at 1186.568 kb on + strand at 1186.568 kb on + strand at 1186.568 kb on + strand at 1186.568 kb on + strand at 1186.568 kb on + strand at 1186.568 kb on + strand at 1186.568 kb on + strand at 1186.568 kb on + strand at 1186.568 kb on + strand at 1186.568 kb on + strand at 1186.568 kb on + strand at 1186.568 kb on + strand at 1186.568 kb on + strand at 1186.569 kb on - strand at 1186.569 kb on - strand at 1186.569 kb on - strand at 1186.569 kb on - strand at 1186.569 kb on - strand at 1186.569 kb on - strand at 1186.569 kb on - strand at 1186.569 kb on - strand at 1186.569 kb on - strand at 1186.569 kb on - strand at 1186.569 kb on - strand at 1186.569 kb on - strand at 1186.569 kb on - strand at 1186.569 kb on - strand at 1186.569 kb on - strand at 1186.569 kb on - strand at 1186.569 kb on - strand at 1186.569 kb on - strand at 1186.569 kb on - strand at 1186.569 kb on - strand at 1186.569 kb on - strand at 1186.569 kb on - strand at 1186.569 kb on - strand at 1186.569 kb on - strand at 1186.569 kb on - strand at 1186.569 kb on - strand at 1186.569 kb on - strand at 1186.569 kb on - strand at 1186.569 kb on - strand at 1186.569 kb on - strand at 1186.569 kb on - strand at 1186.569 kb on - strand at 1186.569 kb on - strand at 1186.569 kb on - strand at 1186.569 kb on - strand at 1186.569 kb on - strand at 1186.569 kb on - strand at 1186.569 kb on - strand at 1186.569 kb on - strand at 1186.569 kb on - strand at 1186.569 kb on - strand at 1186.569 kb on - strand at 1186.569 kb on - strand at 1186.569 kb on - strand at 1186.569 kb on - strand at 1186.569 kb on - strand at 1186.569 kb on - strand at 1186.569 kb on - strand at 1186.569 kb on - strand at 1186.569 kb on - strand at 1186.569 kb on - strand at 1186.569 kb on - strand at 1186.569 kb on - strand at 1186.569 kb on - strand at 1186.572 kb on + strand at 1186.572 kb on + strand at 1186.572 kb on + strand at 1186.572 kb on + strand at 1186.572 kb on + strand at 1186.572 kb on + strand at 1186.572 kb on + strand at 1186.572 kb on + strand at 1186.572 kb on + strand at 1186.572 kb on + strand at 1186.572 kb on + strand at 1186.573 kb on - strand at 1186.573 kb on - strand at 1186.573 kb on - strand at 1186.573 kb on - strand at 1186.573 kb on - strand at 1186.573 kb on - strand at 1186.573 kb on - strand at 1186.573 kb on - strand at 1186.573 kb on - strand at 1186.573 kb on - strand at 1186.573 kb on - strand at 1186.611 kb on - strand at 1186.611 kb on - strand at 1186.611 kb on - strand at 1186.686 kb on + strand at 1186.686 kb on + strand at 1186.686 kb on + strand at 1186.686 kb on + strand at 1186.686 kb on + strand at 1186.686 kb on + strand at 1186.686 kb on + strand at 1186.687 kb on - strand at 1186.687 kb on - strand at 1186.687 kb on - strand at 1186.687 kb on - strand at 1186.687 kb on - strand at 1186.687 kb on - strand at 1186.687 kb on - strand at 1186.687 kb on - strand at 1186.687 kb on - strand at 1186.687 kb on - strand at 1186.841 kb on + strand at 1186.841 kb on + strand at 1186.841 kb on + strand at 1186.841 kb on + strand at 1186.842 kb on - strand at 1186.947 kb on + strand at 1186.947 kb on + strand at 1186.947 kb on + strand at 1186.947 kb on + strand at 1186.947 kb on + strand at 1186.947 kb on + strand at 1186.947 kb on + strand at 1186.947 kb on + strand at 1186.947 kb on + strand at 1186.947 kb on + strand at 1186.947 kb on + strand at 1186.947 kb on + strand at 1186.948 kb on - strand at 1186.948 kb on - strand at 1186.948 kb on - strand at 1186.948 kb on - strand at 1186.948 kb on - strand at 1186.948 kb on - strand at 1186.972 kb on + strand at 1186.972 kb on + strand at 1186.972 kb on + strand at 1186.972 kb on + strand at 1186.972 kb on + strand at 1186.972 kb on + strand at 1186.973 kb on - strand at 1186.973 kb on - strand at 1186.973 kb on - strand at 1186.973 kb on - strand at 1186.973 kb on - strand at 1186.973 kb on - strand at 1186.973 kb on - strand at 1186.973 kb on - strand at 1186.973 kb on - strand at 1187.315 kb on + strand, within LRK54_RS05495 at 1187.315 kb on + strand, within LRK54_RS05495 at 1187.316 kb on - strand, within LRK54_RS05495 at 1187.316 kb on - strand, within LRK54_RS05495 at 1187.316 kb on - strand, within LRK54_RS05495 at 1187.358 kb on - strand, within LRK54_RS05495 at 1187.358 kb on - strand, within LRK54_RS05495 at 1187.449 kb on - strand, within LRK54_RS05495 at 1187.449 kb on - strand, within LRK54_RS05495 at 1187.449 kb on - strand, within LRK54_RS05495 at 1187.449 kb on - strand, within LRK54_RS05495 at 1187.479 kb on + strand, within LRK54_RS05495 at 1187.479 kb on + strand, within LRK54_RS05495 at 1187.479 kb on + strand, within LRK54_RS05495 at 1187.479 kb on + strand, within LRK54_RS05495 at 1187.479 kb on + strand, within LRK54_RS05495 at 1187.479 kb on + strand, within LRK54_RS05495 at 1187.480 kb on - strand, within LRK54_RS05495 at 1187.480 kb on - strand, within LRK54_RS05495 at 1187.480 kb on - strand, within LRK54_RS05495 at 1187.480 kb on - strand, within LRK54_RS05495 at 1187.481 kb on - strand, within LRK54_RS05495 at 1187.508 kb on - strand at 1187.508 kb on - strand at 1187.540 kb on + strand at 1187.540 kb on + strand at 1187.541 kb on - strand at 1187.664 kb on - strand, within LRK54_RS05500 at 1187.726 kb on - strand, within LRK54_RS05500 at 1188.149 kb on + strand at 1188.149 kb on + strand at 1188.149 kb on + strand at 1188.150 kb on - strand at 1188.150 kb on - strand at 1188.302 kb on + strand, within LRK54_RS05505 at 1188.302 kb on + strand, within LRK54_RS05505 at 1188.302 kb on + strand, within LRK54_RS05505 at 1188.302 kb on + strand, within LRK54_RS05505 at 1188.302 kb on + strand, within LRK54_RS05505 at 1188.302 kb on + strand, within LRK54_RS05505 at 1188.302 kb on + strand, within LRK54_RS05505 at 1188.302 kb on + strand, within LRK54_RS05505 at 1188.302 kb on + strand, within LRK54_RS05505 at 1188.302 kb on + strand, within LRK54_RS05505 at 1188.302 kb on + strand, within LRK54_RS05505 at 1188.302 kb on + strand, within LRK54_RS05505 at 1188.302 kb on + strand, within LRK54_RS05505 at 1188.302 kb on + strand, within LRK54_RS05505 at 1188.302 kb on + strand, within LRK54_RS05505 at 1188.302 kb on + strand, within LRK54_RS05505 at 1188.302 kb on + strand, within LRK54_RS05505 at 1188.302 kb on + strand, within LRK54_RS05505 at 1188.303 kb on - strand, within LRK54_RS05505 at 1188.303 kb on - strand, within LRK54_RS05505 at 1188.303 kb on - strand, within LRK54_RS05505 at 1188.303 kb on - strand, within LRK54_RS05505 at 1188.303 kb on - strand, within LRK54_RS05505 at 1188.303 kb on - strand, within LRK54_RS05505 at 1188.303 kb on - strand, within LRK54_RS05505 at 1188.303 kb on - strand, within LRK54_RS05505 at 1188.303 kb on - strand, within LRK54_RS05505 at 1188.303 kb on - strand, within LRK54_RS05505 at 1188.303 kb on - strand, within LRK54_RS05505 at 1188.303 kb on - strand, within LRK54_RS05505 at 1188.303 kb on - strand, within LRK54_RS05505 at 1188.303 kb on - strand, within LRK54_RS05505 at 1188.303 kb on - strand, within LRK54_RS05505 at 1188.303 kb on - strand, within LRK54_RS05505 at 1188.303 kb on - strand, within LRK54_RS05505 at 1188.303 kb on - strand, within LRK54_RS05505 at 1188.389 kb on + strand, within LRK54_RS05505 at 1188.389 kb on + strand, within LRK54_RS05505 at 1188.389 kb on + strand, within LRK54_RS05505 at 1188.389 kb on + strand, within LRK54_RS05505 at 1188.390 kb on - strand, within LRK54_RS05505 at 1188.390 kb on - strand, within LRK54_RS05505 at 1188.390 kb on - strand, within LRK54_RS05505 at 1188.390 kb on - strand, within LRK54_RS05505 at 1188.390 kb on - strand, within LRK54_RS05505 at 1188.390 kb on - strand, within LRK54_RS05505
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Ferric chloride 1.5625 mM remove 1,186,305 + LRK54_RS05490 0.73 -1.0 1,186,305 + LRK54_RS05490 0.73 -0.5 1,186,306 - LRK54_RS05490 0.73 -0.1 1,186,306 - LRK54_RS05490 0.73 +1.6 1,186,306 - LRK54_RS05490 0.73 +0.9 1,186,561 + +0.2 1,186,561 + +0.0 1,186,561 + -1.4 1,186,561 + +0.4 1,186,562 - -0.0 1,186,568 + -3.5 1,186,568 + +0.2 1,186,568 + +1.8 1,186,568 + -1.2 1,186,568 + +0.5 1,186,568 + +0.2 1,186,568 + -1.4 1,186,568 + -0.9 1,186,568 + -1.7 1,186,568 + +1.5 1,186,568 + -1.2 1,186,568 + -1.4 1,186,568 + -1.9 1,186,568 + -2.6 1,186,568 + -0.4 1,186,568 + -1.5 1,186,568 + -0.8 1,186,568 + -0.1 1,186,568 + -0.5 1,186,568 + +0.8 1,186,568 + -1.5 1,186,568 + -1.7 1,186,568 + -3.6 1,186,568 + -0.2 1,186,568 + +0.5 1,186,568 + +0.8 1,186,568 + -0.9 1,186,568 + -0.9 1,186,568 + +0.0 1,186,568 + -2.2 1,186,568 + -0.7 1,186,568 + -2.0 1,186,568 + -0.2 1,186,568 + -0.6 1,186,568 + -1.3 1,186,568 + -0.5 1,186,568 + -1.7 1,186,568 + +1.4 1,186,568 + -0.6 1,186,568 + -1.2 1,186,568 + +0.4 1,186,568 + -0.7 1,186,568 + -0.5 1,186,568 + +0.5 1,186,568 + +0.8 1,186,568 + -1.0 1,186,568 + +0.5 1,186,568 + +0.1 1,186,568 + -0.9 1,186,568 + -0.8 1,186,569 - -0.1 1,186,569 - +1.2 1,186,569 - -0.4 1,186,569 - -1.2 1,186,569 - -1.1 1,186,569 - +0.3 1,186,569 - +1.8 1,186,569 - +0.7 1,186,569 - -2.7 1,186,569 - -0.2 1,186,569 - -1.4 1,186,569 - +1.4 1,186,569 - +2.6 1,186,569 - -1.2 1,186,569 - -1.0 1,186,569 - -2.4 1,186,569 - -0.1 1,186,569 - +0.6 1,186,569 - -0.5 1,186,569 - +0.2 1,186,569 - -0.2 1,186,569 - -1.8 1,186,569 - +0.8 1,186,569 - +0.7 1,186,569 - +0.0 1,186,569 - -1.5 1,186,569 - -1.7 1,186,569 - -1.1 1,186,569 - +0.4 1,186,569 - +0.9 1,186,569 - -1.4 1,186,569 - +0.2 1,186,569 - -1.1 1,186,569 - +0.8 1,186,569 - +0.7 1,186,569 - +0.3 1,186,569 - +0.4 1,186,569 - -0.2 1,186,569 - -1.1 1,186,569 - -0.4 1,186,569 - -1.7 1,186,569 - +1.1 1,186,569 - -0.4 1,186,569 - -1.0 1,186,569 - -1.6 1,186,569 - +0.8 1,186,569 - -2.4 1,186,569 - +0.0 1,186,569 - +0.8 1,186,569 - -2.7 1,186,569 - -1.1 1,186,569 - -1.9 1,186,569 - -0.0 1,186,569 - -0.2 1,186,572 + -1.0 1,186,572 + +0.3 1,186,572 + +0.1 1,186,572 + -1.9 1,186,572 + +1.2 1,186,572 + -0.7 1,186,572 + -0.5 1,186,572 + +1.2 1,186,572 + -0.7 1,186,572 + -1.8 1,186,572 + -0.8 1,186,573 - +1.2 1,186,573 - +0.3 1,186,573 - -0.2 1,186,573 - +0.2 1,186,573 - +0.1 1,186,573 - -0.4 1,186,573 - +0.5 1,186,573 - -1.8 1,186,573 - +0.8 1,186,573 - -0.0 1,186,573 - +1.2 1,186,611 - +1.2 1,186,611 - +0.0 1,186,611 - +2.5 1,186,686 + +0.1 1,186,686 + -0.5 1,186,686 + -0.5 1,186,686 + -3.0 1,186,686 + -1.5 1,186,686 + -1.0 1,186,686 + -0.3 1,186,687 - -1.9 1,186,687 - +0.6 1,186,687 - +0.0 1,186,687 - -2.5 1,186,687 - +0.0 1,186,687 - -1.3 1,186,687 - -2.2 1,186,687 - -1.8 1,186,687 - -0.7 1,186,687 - +1.2 1,186,841 + -2.1 1,186,841 + -0.8 1,186,841 + -0.1 1,186,841 + +0.4 1,186,842 - +1.1 1,186,947 + -0.8 1,186,947 + -2.2 1,186,947 + -1.0 1,186,947 + -2.1 1,186,947 + -3.7 1,186,947 + -1.6 1,186,947 + -2.4 1,186,947 + -1.7 1,186,947 + -2.2 1,186,947 + -2.2 1,186,947 + -2.6 1,186,947 + -0.8 1,186,948 - -2.7 1,186,948 - -1.2 1,186,948 - -1.7 1,186,948 - -0.8 1,186,948 - -0.4 1,186,948 - -1.5 1,186,972 + -0.2 1,186,972 + -0.8 1,186,972 + -3.0 1,186,972 + +0.6 1,186,972 + -4.2 1,186,972 + -0.8 1,186,973 - +1.2 1,186,973 - +1.2 1,186,973 - -0.8 1,186,973 - -3.2 1,186,973 - -1.9 1,186,973 - -2.8 1,186,973 - -0.8 1,186,973 - -1.4 1,186,973 - -2.8 1,187,315 + LRK54_RS05495 0.15 -1.9 1,187,315 + LRK54_RS05495 0.15 +1.8 1,187,316 - LRK54_RS05495 0.16 -1.7 1,187,316 - LRK54_RS05495 0.16 -2.0 1,187,316 - LRK54_RS05495 0.16 -0.8 1,187,358 - LRK54_RS05495 0.34 -2.0 1,187,358 - LRK54_RS05495 0.34 -1.9 1,187,449 - LRK54_RS05495 0.74 -2.2 1,187,449 - LRK54_RS05495 0.74 -1.0 1,187,449 - LRK54_RS05495 0.74 +0.2 1,187,449 - LRK54_RS05495 0.74 +0.1 1,187,479 + LRK54_RS05495 0.87 -2.6 1,187,479 + LRK54_RS05495 0.87 -0.5 1,187,479 + LRK54_RS05495 0.87 -2.9 1,187,479 + LRK54_RS05495 0.87 -2.2 1,187,479 + LRK54_RS05495 0.87 -0.2 1,187,479 + LRK54_RS05495 0.87 -0.2 1,187,480 - LRK54_RS05495 0.88 -0.2 1,187,480 - LRK54_RS05495 0.88 -1.7 1,187,480 - LRK54_RS05495 0.88 -1.7 1,187,480 - LRK54_RS05495 0.88 -2.3 1,187,481 - LRK54_RS05495 0.88 +0.8 1,187,508 - -1.8 1,187,508 - -1.2 1,187,540 + -3.8 1,187,540 + -0.5 1,187,541 - +1.3 1,187,664 - LRK54_RS05500 0.25 -0.2 1,187,726 - LRK54_RS05500 0.39 +0.7 1,188,149 + -0.1 1,188,149 + -0.2 1,188,149 + -2.8 1,188,150 - -1.1 1,188,150 - -1.1 1,188,302 + LRK54_RS05505 0.46 -2.0 1,188,302 + LRK54_RS05505 0.46 -2.1 1,188,302 + LRK54_RS05505 0.46 -0.5 1,188,302 + LRK54_RS05505 0.46 -1.1 1,188,302 + LRK54_RS05505 0.46 +0.2 1,188,302 + LRK54_RS05505 0.46 +1.8 1,188,302 + LRK54_RS05505 0.46 +0.2 1,188,302 + LRK54_RS05505 0.46 -1.5 1,188,302 + LRK54_RS05505 0.46 -0.8 1,188,302 + LRK54_RS05505 0.46 -1.6 1,188,302 + LRK54_RS05505 0.46 -1.0 1,188,302 + LRK54_RS05505 0.46 -2.3 1,188,302 + LRK54_RS05505 0.46 -0.8 1,188,302 + LRK54_RS05505 0.46 -1.5 1,188,302 + LRK54_RS05505 0.46 -1.2 1,188,302 + LRK54_RS05505 0.46 -0.7 1,188,302 + LRK54_RS05505 0.46 -1.0 1,188,302 + LRK54_RS05505 0.46 -3.1 1,188,303 - LRK54_RS05505 0.47 -0.9 1,188,303 - LRK54_RS05505 0.47 -1.1 1,188,303 - LRK54_RS05505 0.47 +0.2 1,188,303 - LRK54_RS05505 0.47 -1.4 1,188,303 - LRK54_RS05505 0.47 -0.7 1,188,303 - LRK54_RS05505 0.47 -1.5 1,188,303 - LRK54_RS05505 0.47 -1.5 1,188,303 - LRK54_RS05505 0.47 -1.6 1,188,303 - LRK54_RS05505 0.47 -1.1 1,188,303 - LRK54_RS05505 0.47 -1.5 1,188,303 - LRK54_RS05505 0.47 -0.4 1,188,303 - LRK54_RS05505 0.47 +0.6 1,188,303 - LRK54_RS05505 0.47 -2.4 1,188,303 - LRK54_RS05505 0.47 -1.2 1,188,303 - LRK54_RS05505 0.47 -1.2 1,188,303 - LRK54_RS05505 0.47 -0.2 1,188,303 - LRK54_RS05505 0.47 -2.0 1,188,303 - LRK54_RS05505 0.47 -0.0 1,188,389 + LRK54_RS05505 0.68 -1.8 1,188,389 + LRK54_RS05505 0.68 +0.3 1,188,389 + LRK54_RS05505 0.68 -0.6 1,188,389 + LRK54_RS05505 0.68 -1.8 1,188,390 - LRK54_RS05505 0.69 -2.2 1,188,390 - LRK54_RS05505 0.69 -1.9 1,188,390 - LRK54_RS05505 0.69 -1.1 1,188,390 - LRK54_RS05505 0.69 -2.4 1,188,390 - LRK54_RS05505 0.69 -1.7 1,188,390 - LRK54_RS05505 0.69 -1.8
Or see this region's nucleotide sequence