Experiment: MoYLS4 with Rifampicin 0.4 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt DvMF_2646 and DvMF_2647 are separated by 312 nucleotides DvMF_2647 and DvMF_2648 are separated by 272 nucleotides DvMF_2648 and DvMF_2649 are separated by 9 nucleotides DvMF_2649 and DvMF_2650 are separated by 178 nucleotides
DvMF_2646: DvMF_2646 - PHP domain protein (RefSeq), at 3,348,664 to 3,349,548
_2646
DvMF_2647: DvMF_2647 - protein of unknown function DUF343 (RefSeq), at 3,349,861 to 3,350,061
_2647
DvMF_2648: DvMF_2648 - heat shock protein Hsp20 (RefSeq), at 3,350,334 to 3,350,747
_2648
DvMF_2649: DvMF_2649 - heat shock protein Hsp20 (RefSeq), at 3,350,757 to 3,351,314
_2649
DvMF_2650: DvMF_2650 - phosphodiesterase (RefSeq), at 3,351,493 to 3,352,041
_2650
Position (kb)
3350
3351 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 3349.405 kb on + strand, within DvMF_2646 at 3349.579 kb on + strand at 3349.587 kb on - strand at 3349.679 kb on + strand at 3349.791 kb on - strand at 3349.846 kb on + strand at 3350.054 kb on - strand at 3350.140 kb on + strand at 3350.186 kb on + strand at 3350.261 kb on + strand at 3350.505 kb on - strand, within DvMF_2648 at 3351.132 kb on - strand, within DvMF_2649 at 3351.397 kb on - strand at 3351.432 kb on + strand at 3351.720 kb on + strand, within DvMF_2650 at 3351.726 kb on - strand, within DvMF_2650
Per-strain Table
Position Strand Gene LocusTag Fraction MoYLS4 with Rifampicin 0.4 mg/ml remove 3,349,405 + DvMF_2646 0.84 -0.0 3,349,579 + -0.3 3,349,587 - -0.5 3,349,679 + -1.2 3,349,791 - -0.5 3,349,846 + -1.1 3,350,054 - +1.9 3,350,140 + -0.7 3,350,186 + -0.4 3,350,261 + -0.4 3,350,505 - DvMF_2648 0.41 -2.5 3,351,132 - DvMF_2649 0.67 -2.2 3,351,397 - -1.7 3,351,432 + +0.1 3,351,720 + DvMF_2650 0.41 -1.4 3,351,726 - DvMF_2650 0.42 -2.9
Or see this region's nucleotide sequence