Experiment: m.b. perchlorate 50 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dshi_2398 and Dshi_2399 are separated by 10 nucleotides Dshi_2399 and Dshi_2400 are separated by 90 nucleotides Dshi_2400 and Dshi_2401 are separated by 175 nucleotides
Dshi_2398: Dshi_2398 - dihydropteroate synthase (RefSeq), at 2,532,406 to 2,533,419
_2398
Dshi_2399: Dshi_2399 - dihydroneopterin aldolase (RefSeq), at 2,533,430 to 2,534,281
_2399
Dshi_2400: Dshi_2400 - cell wall hydrolase SleB (RefSeq), at 2,534,372 to 2,535,064
_2400
Dshi_2401: Dshi_2401 - PEP-utilizing protein (RefSeq), at 2,535,240 to 2,537,828
_2401
Position (kb)
2534
2535
2536 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 2534.402 kb on - strand at 2534.642 kb on - strand, within Dshi_2400 at 2534.787 kb on - strand, within Dshi_2400 at 2534.856 kb on - strand, within Dshi_2400 at 2534.894 kb on - strand, within Dshi_2400 at 2534.919 kb on + strand, within Dshi_2400 at 2534.946 kb on - strand, within Dshi_2400 at 2534.946 kb on - strand, within Dshi_2400 at 2535.082 kb on - strand at 2535.471 kb on - strand at 2535.669 kb on + strand, within Dshi_2401 at 2535.769 kb on + strand, within Dshi_2401 at 2535.838 kb on + strand, within Dshi_2401 at 2535.918 kb on - strand, within Dshi_2401 at 2535.974 kb on + strand, within Dshi_2401
Per-strain Table
Position Strand Gene LocusTag Fraction m.b. perchlorate 50 mM remove 2,534,402 - -1.5 2,534,642 - Dshi_2400 0.39 -0.5 2,534,787 - Dshi_2400 0.60 -0.3 2,534,856 - Dshi_2400 0.70 +0.2 2,534,894 - Dshi_2400 0.75 +0.4 2,534,919 + Dshi_2400 0.79 -3.0 2,534,946 - Dshi_2400 0.83 -0.3 2,534,946 - Dshi_2400 0.83 -1.3 2,535,082 - +0.2 2,535,471 - -1.2 2,535,669 + Dshi_2401 0.17 -0.1 2,535,769 + Dshi_2401 0.20 +0.8 2,535,838 + Dshi_2401 0.23 +0.3 2,535,918 - Dshi_2401 0.26 +0.8 2,535,974 + Dshi_2401 0.28 -0.0
Or see this region's nucleotide sequence