Experiment: MoLS4 with L-Asparagine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt pdxA and DVU2242 are separated by 270 nucleotides DVU2242 and glgB are separated by 285 nucleotides
DVU2241: pdxA - pyridoxal phosphate biosynthetic protein PdxA (TIGR), at 2,334,036 to 2,335,256
pdxA
DVU2242: DVU2242 - asparaginase (EC 3.5.1.1) (from data) , at 2,335,527 to 2,336,474
DVU2242
DVU2243: glgB - 1,4-alpha-glucan branching enzyme (TIGR), at 2,336,760 to 2,338,682
glgB
Position (kb)
2335
2336
2337 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1 at 2334.539 kb on + strand, within pdxA at 2334.566 kb on + strand, within pdxA at 2334.574 kb on - strand, within pdxA at 2334.576 kb on + strand, within pdxA at 2334.658 kb on + strand, within pdxA at 2334.658 kb on + strand, within pdxA at 2334.666 kb on - strand, within pdxA at 2334.666 kb on - strand, within pdxA at 2334.708 kb on - strand, within pdxA at 2334.719 kb on - strand, within pdxA at 2334.779 kb on + strand, within pdxA at 2334.787 kb on - strand, within pdxA at 2334.787 kb on - strand, within pdxA at 2334.797 kb on - strand, within pdxA at 2334.985 kb on + strand, within pdxA at 2334.993 kb on - strand, within pdxA at 2335.037 kb on + strand, within pdxA at 2335.159 kb on - strand at 2335.200 kb on - strand at 2335.223 kb on - strand at 2335.287 kb on + strand at 2335.313 kb on - strand at 2335.337 kb on - strand at 2335.484 kb on + strand at 2335.617 kb on - strand at 2335.733 kb on + strand, within DVU2242 at 2335.760 kb on + strand, within DVU2242 at 2335.761 kb on - strand, within DVU2242 at 2335.827 kb on - strand, within DVU2242 at 2335.832 kb on - strand, within DVU2242 at 2335.839 kb on - strand, within DVU2242 at 2336.002 kb on + strand, within DVU2242 at 2336.004 kb on + strand, within DVU2242 at 2336.004 kb on + strand, within DVU2242 at 2336.031 kb on + strand, within DVU2242 at 2336.089 kb on - strand, within DVU2242 at 2336.089 kb on - strand, within DVU2242 at 2336.176 kb on - strand, within DVU2242 at 2336.211 kb on + strand, within DVU2242 at 2336.234 kb on - strand, within DVU2242 at 2336.285 kb on - strand, within DVU2242 at 2336.546 kb on + strand at 2336.568 kb on + strand at 2336.568 kb on + strand at 2336.638 kb on + strand at 2336.661 kb on - strand at 2336.685 kb on + strand at 2336.766 kb on + strand at 2336.829 kb on + strand at 2336.885 kb on - strand at 2336.911 kb on + strand at 2336.929 kb on - strand at 2337.048 kb on + strand, within glgB at 2337.056 kb on - strand, within glgB at 2337.056 kb on - strand, within glgB at 2337.097 kb on + strand, within glgB at 2337.204 kb on + strand, within glgB at 2337.283 kb on + strand, within glgB at 2337.291 kb on - strand, within glgB at 2337.376 kb on + strand, within glgB at 2337.384 kb on - strand, within glgB at 2337.413 kb on + strand, within glgB at 2337.421 kb on - strand, within glgB
Per-strain Table
Position Strand Gene LocusTag Fraction MoLS4 with L-Asparagine (N) remove 2,334,539 + pdxA DVU2241 0.41 -0.8 2,334,566 + pdxA DVU2241 0.43 -0.2 2,334,574 - pdxA DVU2241 0.44 -1.7 2,334,576 + pdxA DVU2241 0.44 -1.6 2,334,658 + pdxA DVU2241 0.51 +0.3 2,334,658 + pdxA DVU2241 0.51 -0.2 2,334,666 - pdxA DVU2241 0.52 -0.1 2,334,666 - pdxA DVU2241 0.52 -0.7 2,334,708 - pdxA DVU2241 0.55 +0.3 2,334,719 - pdxA DVU2241 0.56 +0.5 2,334,779 + pdxA DVU2241 0.61 -0.9 2,334,787 - pdxA DVU2241 0.62 -0.7 2,334,787 - pdxA DVU2241 0.62 -0.1 2,334,797 - pdxA DVU2241 0.62 -0.1 2,334,985 + pdxA DVU2241 0.78 -1.0 2,334,993 - pdxA DVU2241 0.78 -0.3 2,335,037 + pdxA DVU2241 0.82 -0.6 2,335,159 - -0.7 2,335,200 - -1.1 2,335,223 - -1.9 2,335,287 + -0.3 2,335,313 - -0.2 2,335,337 - -0.8 2,335,484 + -0.3 2,335,617 - -2.9 2,335,733 + DVU2242 0.22 -2.1 2,335,760 + DVU2242 0.25 -0.6 2,335,761 - DVU2242 0.25 -1.3 2,335,827 - DVU2242 0.32 -4.5 2,335,832 - DVU2242 0.32 -4.9 2,335,839 - DVU2242 0.33 -4.1 2,336,002 + DVU2242 0.50 -2.6 2,336,004 + DVU2242 0.50 -0.6 2,336,004 + DVU2242 0.50 -1.6 2,336,031 + DVU2242 0.53 -2.2 2,336,089 - DVU2242 0.59 -4.6 2,336,089 - DVU2242 0.59 -2.6 2,336,176 - DVU2242 0.68 -5.0 2,336,211 + DVU2242 0.72 -2.7 2,336,234 - DVU2242 0.75 -4.1 2,336,285 - DVU2242 0.80 -3.6 2,336,546 + +0.1 2,336,568 + -1.1 2,336,568 + -3.2 2,336,638 + -1.4 2,336,661 - -0.0 2,336,685 + +0.3 2,336,766 + -0.3 2,336,829 + +1.0 2,336,885 - +0.4 2,336,911 + -0.3 2,336,929 - -0.4 2,337,048 + glgB DVU2243 0.15 +0.2 2,337,056 - glgB DVU2243 0.15 +0.6 2,337,056 - glgB DVU2243 0.15 -1.4 2,337,097 + glgB DVU2243 0.18 -0.5 2,337,204 + glgB DVU2243 0.23 -0.7 2,337,283 + glgB DVU2243 0.27 -0.4 2,337,291 - glgB DVU2243 0.28 -0.7 2,337,376 + glgB DVU2243 0.32 -0.5 2,337,384 - glgB DVU2243 0.32 -0.3 2,337,413 + glgB DVU2243 0.34 -1.7 2,337,421 - glgB DVU2243 0.34 -1.0
Or see this region's nucleotide sequence