Experiment: PYE with Vancomycin 0.007 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CCNA_01579 and CCNA_01580 are separated by 71 nucleotides CCNA_01580 and CCNA_01581 are separated by 108 nucleotides CCNA_01581 and CCNA_01582 are separated by 114 nucleotides CCNA_01582 and CCNA_01583 are separated by 69 nucleotides
CCNA_01579: CCNA_01579 - cell wall degradation protein, at 1,695,303 to 1,696,811
_01579
CCNA_01580: CCNA_01580 - M15 superfamily membrane peptidase, at 1,696,883 to 1,697,533
_01580
CCNA_01581: CCNA_01581 - hypothetical protein, at 1,697,642 to 1,697,974
_01581
CCNA_01582: CCNA_01582 - Glutathione-dependent formaldehyde-activating enzyme, at 1,698,089 to 1,698,451
_01582
CCNA_01583: CCNA_01583 - phosphate-binding protein, at 1,698,521 to 1,699,561
_01583
Position (kb)
1696
1697
1698 Strain fitness (log2 ratio)
-2
-1
0
1
2
3
4 at 1695.894 kb on - strand, within CCNA_01579 at 1695.904 kb on - strand, within CCNA_01579 at 1695.948 kb on + strand, within CCNA_01579 at 1695.961 kb on - strand, within CCNA_01579 at 1695.996 kb on + strand, within CCNA_01579 at 1695.996 kb on + strand, within CCNA_01579 at 1695.996 kb on + strand, within CCNA_01579 at 1695.996 kb on + strand, within CCNA_01579 at 1695.996 kb on + strand, within CCNA_01579 at 1695.996 kb on + strand, within CCNA_01579 at 1695.996 kb on + strand, within CCNA_01579 at 1695.996 kb on + strand, within CCNA_01579 at 1695.996 kb on + strand, within CCNA_01579 at 1695.997 kb on + strand, within CCNA_01579 at 1696.001 kb on + strand, within CCNA_01579 at 1696.001 kb on + strand, within CCNA_01579 at 1696.001 kb on + strand, within CCNA_01579 at 1696.001 kb on + strand, within CCNA_01579 at 1696.001 kb on + strand, within CCNA_01579 at 1696.001 kb on + strand, within CCNA_01579 at 1696.004 kb on - strand, within CCNA_01579 at 1696.009 kb on - strand, within CCNA_01579 at 1696.020 kb on + strand, within CCNA_01579 at 1696.044 kb on - strand, within CCNA_01579 at 1696.077 kb on + strand, within CCNA_01579 at 1696.077 kb on + strand, within CCNA_01579 at 1696.077 kb on + strand, within CCNA_01579 at 1696.082 kb on + strand, within CCNA_01579 at 1696.085 kb on - strand, within CCNA_01579 at 1696.085 kb on - strand, within CCNA_01579 at 1696.085 kb on - strand, within CCNA_01579 at 1696.107 kb on + strand, within CCNA_01579 at 1696.153 kb on - strand, within CCNA_01579 at 1696.154 kb on - strand, within CCNA_01579 at 1696.237 kb on + strand, within CCNA_01579 at 1696.242 kb on + strand, within CCNA_01579 at 1696.319 kb on - strand, within CCNA_01579 at 1696.346 kb on + strand, within CCNA_01579 at 1696.346 kb on + strand, within CCNA_01579 at 1696.364 kb on + strand, within CCNA_01579 at 1696.417 kb on - strand, within CCNA_01579 at 1696.444 kb on - strand, within CCNA_01579 at 1696.498 kb on + strand, within CCNA_01579 at 1696.506 kb on - strand, within CCNA_01579 at 1696.512 kb on - strand, within CCNA_01579 at 1696.576 kb on + strand, within CCNA_01579 at 1696.595 kb on + strand, within CCNA_01579 at 1696.675 kb on + strand at 1696.675 kb on + strand at 1696.675 kb on + strand at 1696.679 kb on - strand at 1696.712 kb on + strand at 1696.712 kb on + strand at 1696.720 kb on - strand at 1696.770 kb on + strand at 1696.837 kb on + strand at 1696.837 kb on + strand at 1696.837 kb on + strand at 1696.837 kb on + strand at 1696.849 kb on + strand at 1696.990 kb on + strand, within CCNA_01580 at 1696.995 kb on - strand, within CCNA_01580 at 1696.997 kb on + strand, within CCNA_01580 at 1697.053 kb on - strand, within CCNA_01580 at 1697.065 kb on + strand, within CCNA_01580 at 1697.091 kb on + strand, within CCNA_01580 at 1697.091 kb on + strand, within CCNA_01580 at 1697.091 kb on + strand, within CCNA_01580 at 1697.094 kb on - strand, within CCNA_01580 at 1697.098 kb on + strand, within CCNA_01580 at 1697.099 kb on - strand, within CCNA_01580 at 1697.147 kb on - strand, within CCNA_01580 at 1697.159 kb on + strand, within CCNA_01580 at 1697.182 kb on + strand, within CCNA_01580 at 1697.182 kb on + strand, within CCNA_01580 at 1697.216 kb on + strand, within CCNA_01580 at 1697.238 kb on + strand, within CCNA_01580 at 1697.246 kb on - strand, within CCNA_01580 at 1697.246 kb on - strand, within CCNA_01580 at 1697.246 kb on - strand, within CCNA_01580 at 1697.270 kb on + strand, within CCNA_01580 at 1697.277 kb on + strand, within CCNA_01580 at 1697.278 kb on - strand, within CCNA_01580 at 1697.278 kb on - strand, within CCNA_01580 at 1697.545 kb on + strand at 1697.584 kb on + strand at 1697.612 kb on - strand at 1697.634 kb on + strand at 1697.634 kb on + strand at 1697.644 kb on - strand at 1697.644 kb on - strand at 1697.651 kb on + strand at 1697.669 kb on + strand at 1697.760 kb on + strand, within CCNA_01581 at 1697.773 kb on - strand, within CCNA_01581 at 1697.829 kb on - strand, within CCNA_01581 at 1697.850 kb on - strand, within CCNA_01581 at 1697.850 kb on - strand, within CCNA_01581 at 1698.002 kb on - strand at 1698.018 kb on + strand at 1698.018 kb on + strand at 1698.126 kb on + strand, within CCNA_01582 at 1698.136 kb on + strand, within CCNA_01582 at 1698.237 kb on - strand, within CCNA_01582 at 1698.389 kb on - strand, within CCNA_01582 at 1698.396 kb on - strand, within CCNA_01582 at 1698.406 kb on + strand, within CCNA_01582 at 1698.421 kb on + strand at 1698.429 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction PYE with Vancomycin 0.007 mg/ml remove 1,695,894 - CCNA_01579 0.39 +0.8 1,695,904 - CCNA_01579 0.40 +2.8 1,695,948 + CCNA_01579 0.43 +0.2 1,695,961 - CCNA_01579 0.44 +0.2 1,695,996 + CCNA_01579 0.46 +0.4 1,695,996 + CCNA_01579 0.46 +0.1 1,695,996 + CCNA_01579 0.46 -0.5 1,695,996 + CCNA_01579 0.46 -2.3 1,695,996 + CCNA_01579 0.46 -0.2 1,695,996 + CCNA_01579 0.46 -0.5 1,695,996 + CCNA_01579 0.46 -0.9 1,695,996 + CCNA_01579 0.46 -1.3 1,695,996 + CCNA_01579 0.46 +1.2 1,695,997 + CCNA_01579 0.46 +0.8 1,696,001 + CCNA_01579 0.46 -0.8 1,696,001 + CCNA_01579 0.46 +0.4 1,696,001 + CCNA_01579 0.46 -0.8 1,696,001 + CCNA_01579 0.46 -0.6 1,696,001 + CCNA_01579 0.46 +1.2 1,696,001 + CCNA_01579 0.46 +1.2 1,696,004 - CCNA_01579 0.46 +1.8 1,696,009 - CCNA_01579 0.47 -2.1 1,696,020 + CCNA_01579 0.48 -0.6 1,696,044 - CCNA_01579 0.49 +1.8 1,696,077 + CCNA_01579 0.51 +0.8 1,696,077 + CCNA_01579 0.51 -1.6 1,696,077 + CCNA_01579 0.51 +0.8 1,696,082 + CCNA_01579 0.52 +0.2 1,696,085 - CCNA_01579 0.52 +0.0 1,696,085 - CCNA_01579 0.52 -0.5 1,696,085 - CCNA_01579 0.52 +0.8 1,696,107 + CCNA_01579 0.53 +1.8 1,696,153 - CCNA_01579 0.56 +0.5 1,696,154 - CCNA_01579 0.56 -2.0 1,696,237 + CCNA_01579 0.62 -1.6 1,696,242 + CCNA_01579 0.62 +0.5 1,696,319 - CCNA_01579 0.67 +2.8 1,696,346 + CCNA_01579 0.69 +1.0 1,696,346 + CCNA_01579 0.69 -0.1 1,696,364 + CCNA_01579 0.70 +0.2 1,696,417 - CCNA_01579 0.74 -0.8 1,696,444 - CCNA_01579 0.76 +0.7 1,696,498 + CCNA_01579 0.79 -1.6 1,696,506 - CCNA_01579 0.80 -1.5 1,696,512 - CCNA_01579 0.80 -1.9 1,696,576 + CCNA_01579 0.84 -2.1 1,696,595 + CCNA_01579 0.86 -0.0 1,696,675 + -0.5 1,696,675 + -0.6 1,696,675 + -0.7 1,696,679 - +2.8 1,696,712 + -1.1 1,696,712 + -0.2 1,696,720 - -2.7 1,696,770 + -0.3 1,696,837 + +0.2 1,696,837 + +2.8 1,696,837 + +0.4 1,696,837 + -1.7 1,696,849 + +1.3 1,696,990 + CCNA_01580 0.16 +3.7 1,696,995 - CCNA_01580 0.17 -0.1 1,696,997 + CCNA_01580 0.18 +0.3 1,697,053 - CCNA_01580 0.26 -1.3 1,697,065 + CCNA_01580 0.28 +1.1 1,697,091 + CCNA_01580 0.32 +3.2 1,697,091 + CCNA_01580 0.32 +4.2 1,697,091 + CCNA_01580 0.32 +0.8 1,697,094 - CCNA_01580 0.32 +0.5 1,697,098 + CCNA_01580 0.33 +0.5 1,697,099 - CCNA_01580 0.33 +1.4 1,697,147 - CCNA_01580 0.41 -0.9 1,697,159 + CCNA_01580 0.42 +1.0 1,697,182 + CCNA_01580 0.46 +0.1 1,697,182 + CCNA_01580 0.46 +0.1 1,697,216 + CCNA_01580 0.51 +4.1 1,697,238 + CCNA_01580 0.55 +0.5 1,697,246 - CCNA_01580 0.56 +0.8 1,697,246 - CCNA_01580 0.56 +0.2 1,697,246 - CCNA_01580 0.56 +0.8 1,697,270 + CCNA_01580 0.59 +0.7 1,697,277 + CCNA_01580 0.61 +0.2 1,697,278 - CCNA_01580 0.61 +2.8 1,697,278 - CCNA_01580 0.61 +2.8 1,697,545 + +0.2 1,697,584 + -0.7 1,697,612 - +1.8 1,697,634 + -0.3 1,697,634 + -1.4 1,697,644 - +0.4 1,697,644 - -0.5 1,697,651 + -0.5 1,697,669 + -1.2 1,697,760 + CCNA_01581 0.35 +1.2 1,697,773 - CCNA_01581 0.39 +1.2 1,697,829 - CCNA_01581 0.56 +0.2 1,697,850 - CCNA_01581 0.62 -0.8 1,697,850 - CCNA_01581 0.62 -0.2 1,698,002 - -1.5 1,698,018 + -1.0 1,698,018 + -0.6 1,698,126 + CCNA_01582 0.10 -1.4 1,698,136 + CCNA_01582 0.13 +0.0 1,698,237 - CCNA_01582 0.41 +0.2 1,698,389 - CCNA_01582 0.83 +1.2 1,698,396 - CCNA_01582 0.85 +1.1 1,698,406 + CCNA_01582 0.87 -0.9 1,698,421 + +0.2 1,698,429 - -0.2
Or see this region's nucleotide sequence