Experiment: PYE with Vancomycin 0.007 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CCNA_00413 and CCNA_00414 are separated by 20 nucleotides CCNA_00414 and CCNA_00415 are separated by 258 nucleotides CCNA_00415 and CCNA_00416 are separated by 140 nucleotides CCNA_00416 and CCNA_00417 are separated by 3 nucleotides
CCNA_00413: CCNA_00413 - poly(A) polymerase/tRNA-nucleotidyltransferase, at 426,379 to 427,632
_00413
CCNA_00414: CCNA_00414 - type 1 glutamine amidotransferase (GATase1) domain protein, at 427,653 to 428,354
_00414
CCNA_00415: CCNA_00415 - hypothetical protein, at 428,613 to 429,584
_00415
CCNA_00416: CCNA_00416 - hypothetical protein, at 429,725 to 430,123
_00416
CCNA_00417: CCNA_00417 - hypothetical protein, at 430,127 to 430,498
_00417
Position (kb) 428
429
430 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3
4 at 427.617 kb on + strand at 427.617 kb on + strand at 427.625 kb on - strand at 427.625 kb on - strand at 427.625 kb on - strand at 427.625 kb on - strand at 427.631 kb on - strand at 427.693 kb on + strand at 427.703 kb on + strand at 427.739 kb on + strand, within CCNA_00414 at 427.744 kb on + strand, within CCNA_00414 at 427.781 kb on - strand, within CCNA_00414 at 427.844 kb on + strand, within CCNA_00414 at 427.859 kb on + strand, within CCNA_00414 at 427.859 kb on + strand, within CCNA_00414 at 427.867 kb on - strand, within CCNA_00414 at 427.867 kb on - strand, within CCNA_00414 at 427.867 kb on - strand, within CCNA_00414 at 427.867 kb on - strand, within CCNA_00414 at 427.925 kb on - strand, within CCNA_00414 at 427.927 kb on + strand, within CCNA_00414 at 427.940 kb on + strand, within CCNA_00414 at 427.948 kb on - strand, within CCNA_00414 at 427.968 kb on + strand, within CCNA_00414 at 427.979 kb on + strand, within CCNA_00414 at 427.985 kb on + strand, within CCNA_00414 at 427.985 kb on + strand, within CCNA_00414 at 427.985 kb on + strand, within CCNA_00414 at 427.985 kb on + strand, within CCNA_00414 at 428.014 kb on - strand, within CCNA_00414 at 428.014 kb on - strand, within CCNA_00414 at 428.073 kb on + strand, within CCNA_00414 at 428.078 kb on + strand, within CCNA_00414 at 428.078 kb on + strand, within CCNA_00414 at 428.078 kb on + strand, within CCNA_00414 at 428.086 kb on - strand, within CCNA_00414 at 428.093 kb on - strand, within CCNA_00414 at 428.119 kb on - strand, within CCNA_00414 at 428.181 kb on - strand, within CCNA_00414 at 428.225 kb on - strand, within CCNA_00414 at 428.230 kb on - strand, within CCNA_00414 at 428.241 kb on + strand, within CCNA_00414 at 428.243 kb on + strand, within CCNA_00414 at 428.243 kb on + strand, within CCNA_00414 at 428.243 kb on + strand, within CCNA_00414 at 428.244 kb on + strand, within CCNA_00414 at 428.244 kb on + strand, within CCNA_00414 at 428.251 kb on - strand, within CCNA_00414 at 428.252 kb on - strand, within CCNA_00414 at 428.279 kb on - strand, within CCNA_00414 at 428.280 kb on + strand, within CCNA_00414 at 428.328 kb on - strand at 428.342 kb on + strand at 428.343 kb on + strand at 428.343 kb on + strand at 428.349 kb on + strand at 428.384 kb on + strand at 428.385 kb on + strand at 428.385 kb on + strand at 428.385 kb on + strand at 428.390 kb on + strand at 428.393 kb on - strand at 428.393 kb on - strand at 428.398 kb on - strand at 428.431 kb on + strand at 428.454 kb on + strand at 428.507 kb on - strand at 428.530 kb on - strand at 428.569 kb on + strand at 428.569 kb on + strand at 428.577 kb on - strand at 428.582 kb on - strand at 428.591 kb on - strand at 428.593 kb on + strand at 428.593 kb on + strand at 428.601 kb on - strand at 428.625 kb on - strand at 428.646 kb on - strand at 428.667 kb on + strand at 428.672 kb on + strand at 428.675 kb on - strand at 428.695 kb on + strand at 428.703 kb on - strand at 428.723 kb on - strand, within CCNA_00415 at 428.723 kb on - strand, within CCNA_00415 at 428.774 kb on + strand, within CCNA_00415 at 428.779 kb on + strand, within CCNA_00415 at 428.786 kb on + strand, within CCNA_00415 at 428.860 kb on + strand, within CCNA_00415 at 428.885 kb on - strand, within CCNA_00415 at 428.898 kb on - strand, within CCNA_00415 at 428.975 kb on - strand, within CCNA_00415 at 428.976 kb on + strand, within CCNA_00415 at 428.976 kb on + strand, within CCNA_00415 at 428.976 kb on + strand, within CCNA_00415 at 428.984 kb on - strand, within CCNA_00415 at 428.984 kb on - strand, within CCNA_00415 at 428.984 kb on - strand, within CCNA_00415 at 428.984 kb on - strand, within CCNA_00415 at 428.995 kb on + strand, within CCNA_00415 at 429.000 kb on + strand, within CCNA_00415 at 429.003 kb on - strand, within CCNA_00415 at 429.008 kb on - strand, within CCNA_00415 at 429.012 kb on + strand, within CCNA_00415 at 429.020 kb on - strand, within CCNA_00415 at 429.030 kb on - strand, within CCNA_00415 at 429.031 kb on - strand, within CCNA_00415 at 429.032 kb on + strand, within CCNA_00415 at 429.062 kb on - strand, within CCNA_00415 at 429.113 kb on + strand, within CCNA_00415 at 429.179 kb on - strand, within CCNA_00415 at 429.179 kb on - strand, within CCNA_00415 at 429.206 kb on - strand, within CCNA_00415 at 429.266 kb on - strand, within CCNA_00415 at 429.269 kb on - strand, within CCNA_00415 at 429.329 kb on + strand, within CCNA_00415 at 429.336 kb on + strand, within CCNA_00415 at 429.355 kb on + strand, within CCNA_00415 at 429.407 kb on - strand, within CCNA_00415 at 429.452 kb on + strand, within CCNA_00415 at 429.475 kb on + strand, within CCNA_00415 at 429.492 kb on - strand at 429.499 kb on + strand at 429.526 kb on - strand at 429.550 kb on + strand at 429.553 kb on - strand at 429.564 kb on + strand at 429.579 kb on + strand at 429.579 kb on + strand at 429.579 kb on + strand at 429.587 kb on - strand at 429.587 kb on - strand at 429.587 kb on - strand at 429.602 kb on - strand at 429.618 kb on - strand at 429.645 kb on - strand at 429.663 kb on - strand at 429.677 kb on - strand at 429.708 kb on + strand at 429.716 kb on - strand at 429.731 kb on - strand at 429.735 kb on - strand at 429.779 kb on - strand, within CCNA_00416 at 429.827 kb on - strand, within CCNA_00416 at 429.845 kb on + strand, within CCNA_00416 at 429.872 kb on + strand, within CCNA_00416 at 429.907 kb on - strand, within CCNA_00416 at 429.917 kb on - strand, within CCNA_00416 at 429.929 kb on - strand, within CCNA_00416 at 429.931 kb on + strand, within CCNA_00416 at 429.931 kb on + strand, within CCNA_00416 at 429.956 kb on + strand, within CCNA_00416 at 429.961 kb on - strand, within CCNA_00416 at 429.961 kb on - strand, within CCNA_00416 at 429.962 kb on - strand, within CCNA_00416 at 429.962 kb on - strand, within CCNA_00416 at 429.974 kb on - strand, within CCNA_00416 at 429.974 kb on - strand, within CCNA_00416 at 429.976 kb on + strand, within CCNA_00416 at 429.976 kb on + strand, within CCNA_00416 at 429.984 kb on - strand, within CCNA_00416 at 430.009 kb on - strand, within CCNA_00416 at 430.025 kb on + strand, within CCNA_00416 at 430.025 kb on + strand, within CCNA_00416 at 430.033 kb on - strand, within CCNA_00416 at 430.051 kb on - strand, within CCNA_00416 at 430.066 kb on - strand, within CCNA_00416 at 430.067 kb on - strand, within CCNA_00416 at 430.074 kb on + strand, within CCNA_00416 at 430.081 kb on - strand, within CCNA_00416 at 430.085 kb on + strand at 430.103 kb on + strand at 430.124 kb on + strand at 430.132 kb on - strand at 430.133 kb on - strand at 430.133 kb on - strand at 430.147 kb on + strand at 430.147 kb on - strand at 430.147 kb on - strand at 430.152 kb on + strand at 430.152 kb on + strand at 430.152 kb on + strand at 430.157 kb on + strand at 430.160 kb on - strand at 430.160 kb on - strand at 430.160 kb on - strand at 430.160 kb on - strand at 430.160 kb on - strand at 430.160 kb on - strand at 430.207 kb on + strand, within CCNA_00417 at 430.207 kb on + strand, within CCNA_00417 at 430.207 kb on - strand, within CCNA_00417 at 430.209 kb on - strand, within CCNA_00417 at 430.249 kb on - strand, within CCNA_00417 at 430.256 kb on + strand, within CCNA_00417 at 430.263 kb on + strand, within CCNA_00417 at 430.263 kb on + strand at 430.266 kb on - strand, within CCNA_00417 at 430.269 kb on - strand, within CCNA_00417 at 430.271 kb on - strand, within CCNA_00417 at 430.299 kb on - strand, within CCNA_00417 at 430.315 kb on + strand, within CCNA_00417 at 430.319 kb on - strand, within CCNA_00417 at 430.361 kb on + strand, within CCNA_00417 at 430.374 kb on - strand, within CCNA_00417 at 430.385 kb on + strand, within CCNA_00417 at 430.436 kb on + strand, within CCNA_00417 at 430.436 kb on + strand, within CCNA_00417 at 430.444 kb on - strand, within CCNA_00417 at 430.486 kb on - strand at 430.501 kb on - strand at 430.510 kb on + strand at 430.548 kb on - strand at 430.577 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction PYE with Vancomycin 0.007 mg/ml remove 427,617 + -0.6 427,617 + -0.2 427,625 - +1.5 427,625 - +0.9 427,625 - -1.0 427,625 - -0.7 427,631 - -2.5 427,693 + +0.6 427,703 + -0.3 427,739 + CCNA_00414 0.12 -1.4 427,744 + CCNA_00414 0.13 +0.5 427,781 - CCNA_00414 0.18 -0.6 427,844 + CCNA_00414 0.27 -0.5 427,859 + CCNA_00414 0.29 +0.1 427,859 + CCNA_00414 0.29 -0.5 427,867 - CCNA_00414 0.30 -0.1 427,867 - CCNA_00414 0.30 +0.7 427,867 - CCNA_00414 0.30 +0.5 427,867 - CCNA_00414 0.30 -1.3 427,925 - CCNA_00414 0.39 -2.0 427,927 + CCNA_00414 0.39 -0.5 427,940 + CCNA_00414 0.41 +0.0 427,948 - CCNA_00414 0.42 +0.9 427,968 + CCNA_00414 0.45 -1.1 427,979 + CCNA_00414 0.46 +0.3 427,985 + CCNA_00414 0.47 -0.6 427,985 + CCNA_00414 0.47 +1.3 427,985 + CCNA_00414 0.47 +1.0 427,985 + CCNA_00414 0.47 +1.4 428,014 - CCNA_00414 0.51 +0.7 428,014 - CCNA_00414 0.51 +0.2 428,073 + CCNA_00414 0.60 -0.4 428,078 + CCNA_00414 0.61 -0.7 428,078 + CCNA_00414 0.61 +0.0 428,078 + CCNA_00414 0.61 +1.7 428,086 - CCNA_00414 0.62 +0.5 428,093 - CCNA_00414 0.63 -0.9 428,119 - CCNA_00414 0.66 -0.3 428,181 - CCNA_00414 0.75 -0.3 428,225 - CCNA_00414 0.81 +0.5 428,230 - CCNA_00414 0.82 +0.2 428,241 + CCNA_00414 0.84 +1.4 428,243 + CCNA_00414 0.84 -0.9 428,243 + CCNA_00414 0.84 +1.0 428,243 + CCNA_00414 0.84 +1.0 428,244 + CCNA_00414 0.84 -0.0 428,244 + CCNA_00414 0.84 +4.5 428,251 - CCNA_00414 0.85 -2.2 428,252 - CCNA_00414 0.85 +1.3 428,279 - CCNA_00414 0.89 -2.0 428,280 + CCNA_00414 0.89 +0.2 428,328 - -0.3 428,342 + +0.5 428,343 + +0.6 428,343 + +0.7 428,349 + -0.4 428,384 + +0.6 428,385 + +0.6 428,385 + +0.7 428,385 + -0.1 428,390 + +1.9 428,393 - +0.7 428,393 - -0.1 428,398 - -0.5 428,431 + -1.9 428,454 + -1.5 428,507 - -0.2 428,530 - -0.3 428,569 + +0.4 428,569 + +0.2 428,577 - -1.4 428,582 - -1.4 428,591 - +0.7 428,593 + -1.3 428,593 + -1.5 428,601 - +0.5 428,625 - -0.5 428,646 - -2.9 428,667 + +0.9 428,672 + +0.5 428,675 - +0.5 428,695 + -1.4 428,703 - -0.6 428,723 - CCNA_00415 0.11 +0.0 428,723 - CCNA_00415 0.11 +0.2 428,774 + CCNA_00415 0.17 -0.4 428,779 + CCNA_00415 0.17 -0.3 428,786 + CCNA_00415 0.18 -0.5 428,860 + CCNA_00415 0.25 +0.9 428,885 - CCNA_00415 0.28 -0.6 428,898 - CCNA_00415 0.29 -1.1 428,975 - CCNA_00415 0.37 -0.7 428,976 + CCNA_00415 0.37 -0.2 428,976 + CCNA_00415 0.37 -1.3 428,976 + CCNA_00415 0.37 -1.6 428,984 - CCNA_00415 0.38 -0.9 428,984 - CCNA_00415 0.38 -0.1 428,984 - CCNA_00415 0.38 -1.0 428,984 - CCNA_00415 0.38 -2.8 428,995 + CCNA_00415 0.39 -0.5 429,000 + CCNA_00415 0.40 -2.1 429,003 - CCNA_00415 0.40 -1.4 429,008 - CCNA_00415 0.41 -2.1 429,012 + CCNA_00415 0.41 -2.5 429,020 - CCNA_00415 0.42 -0.8 429,030 - CCNA_00415 0.43 -0.9 429,031 - CCNA_00415 0.43 -0.5 429,032 + CCNA_00415 0.43 -0.8 429,062 - CCNA_00415 0.46 -1.1 429,113 + CCNA_00415 0.51 -0.5 429,179 - CCNA_00415 0.58 -0.6 429,179 - CCNA_00415 0.58 -0.3 429,206 - CCNA_00415 0.61 -1.1 429,266 - CCNA_00415 0.67 +1.7 429,269 - CCNA_00415 0.67 -0.7 429,329 + CCNA_00415 0.74 +1.3 429,336 + CCNA_00415 0.74 -0.3 429,355 + CCNA_00415 0.76 -1.6 429,407 - CCNA_00415 0.82 +1.1 429,452 + CCNA_00415 0.86 -1.2 429,475 + CCNA_00415 0.89 +0.6 429,492 - -0.2 429,499 + -1.2 429,526 - -0.3 429,550 + +1.8 429,553 - +0.5 429,564 + -0.1 429,579 + +1.0 429,579 + -0.9 429,579 + -0.4 429,587 - -1.5 429,587 - -0.9 429,587 - -0.3 429,602 - +0.3 429,618 - +0.6 429,645 - +1.0 429,663 - -1.3 429,677 - +0.3 429,708 + -0.6 429,716 - +1.4 429,731 - -0.9 429,735 - -1.7 429,779 - CCNA_00416 0.14 +0.6 429,827 - CCNA_00416 0.26 -0.7 429,845 + CCNA_00416 0.30 +1.0 429,872 + CCNA_00416 0.37 -0.4 429,907 - CCNA_00416 0.46 -0.2 429,917 - CCNA_00416 0.48 -0.9 429,929 - CCNA_00416 0.51 -1.2 429,931 + CCNA_00416 0.52 -0.2 429,931 + CCNA_00416 0.52 -0.9 429,956 + CCNA_00416 0.58 -0.3 429,961 - CCNA_00416 0.59 +0.4 429,961 - CCNA_00416 0.59 +0.3 429,962 - CCNA_00416 0.59 +1.5 429,962 - CCNA_00416 0.59 +1.0 429,974 - CCNA_00416 0.62 +0.4 429,974 - CCNA_00416 0.62 -0.6 429,976 + CCNA_00416 0.63 -0.0 429,976 + CCNA_00416 0.63 +2.0 429,984 - CCNA_00416 0.65 +1.7 430,009 - CCNA_00416 0.71 +0.5 430,025 + CCNA_00416 0.75 +1.0 430,025 + CCNA_00416 0.75 -1.2 430,033 - CCNA_00416 0.77 -0.5 430,051 - CCNA_00416 0.82 -0.7 430,066 - CCNA_00416 0.85 +0.4 430,067 - CCNA_00416 0.86 +1.2 430,074 + CCNA_00416 0.87 +3.0 430,081 - CCNA_00416 0.89 -1.3 430,085 + +1.0 430,103 + -0.9 430,124 + +1.0 430,132 - -0.2 430,133 - -1.7 430,133 - +3.5 430,147 + -2.2 430,147 - +0.3 430,147 - +0.2 430,152 + -0.9 430,152 + -1.2 430,152 + -0.5 430,157 + -0.5 430,160 - +0.4 430,160 - -0.1 430,160 - -0.5 430,160 - -1.2 430,160 - +1.0 430,160 - -0.3 430,207 + CCNA_00417 0.22 +1.5 430,207 + CCNA_00417 0.22 -1.7 430,207 - CCNA_00417 0.22 -1.0 430,209 - CCNA_00417 0.22 -0.0 430,249 - CCNA_00417 0.33 +1.0 430,256 + CCNA_00417 0.35 -1.2 430,263 + CCNA_00417 0.37 +0.2 430,263 + +1.0 430,266 - CCNA_00417 0.37 -0.4 430,269 - CCNA_00417 0.38 -0.9 430,271 - CCNA_00417 0.39 -1.3 430,299 - CCNA_00417 0.46 -0.2 430,315 + CCNA_00417 0.51 -0.3 430,319 - CCNA_00417 0.52 +0.1 430,361 + CCNA_00417 0.63 +0.3 430,374 - CCNA_00417 0.66 -0.0 430,385 + CCNA_00417 0.69 +1.0 430,436 + CCNA_00417 0.83 +1.5 430,436 + CCNA_00417 0.83 +0.2 430,444 - CCNA_00417 0.85 -0.0 430,486 - -0.9 430,501 - -2.1 430,510 + -0.9 430,548 - -0.0 430,577 + -0.6
Or see this region's nucleotide sequence