Experiment: methylglyoxal 0.001 vol%
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_1972 and Shew_1973 are separated by 20 nucleotides Shew_1973 and Shew_1974 are separated by 159 nucleotides Shew_1974 and Shew_1975 are separated by 235 nucleotides
Shew_1972: Shew_1972 - FMN-binding domain-containing protein (RefSeq), at 2,279,858 to 2,281,207
_1972
Shew_1973: Shew_1973 - porin (RefSeq), at 2,281,228 to 2,282,277
_1973
Shew_1974: Shew_1974 - hypothetical protein (RefSeq), at 2,282,437 to 2,283,003
_1974
Shew_1975: Shew_1975 - putative manganese-dependent inorganic pyrophosphatase (RefSeq), at 2,283,239 to 2,284,159
_1975
Position (kb)
2281
2282
2283 Strain fitness (log2 ratio)
-1
0
1
2 at 2280.572 kb on + strand, within Shew_1972 at 2280.621 kb on + strand, within Shew_1972 at 2281.099 kb on - strand at 2281.125 kb on + strand at 2281.434 kb on - strand, within Shew_1973 at 2281.487 kb on - strand, within Shew_1973 at 2281.801 kb on + strand, within Shew_1973 at 2281.809 kb on - strand, within Shew_1973 at 2281.846 kb on - strand, within Shew_1973 at 2281.878 kb on + strand, within Shew_1973 at 2281.886 kb on - strand, within Shew_1973 at 2281.886 kb on - strand, within Shew_1973 at 2282.180 kb on - strand at 2282.383 kb on - strand at 2282.383 kb on - strand at 2282.582 kb on + strand, within Shew_1974 at 2282.733 kb on + strand, within Shew_1974 at 2282.980 kb on + strand at 2282.980 kb on + strand at 2283.029 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction methylglyoxal 0.001 vol% remove 2,280,572 + Shew_1972 0.53 -0.5 2,280,621 + Shew_1972 0.57 +0.1 2,281,099 - -0.2 2,281,125 + +0.5 2,281,434 - Shew_1973 0.20 +2.2 2,281,487 - Shew_1973 0.25 +0.0 2,281,801 + Shew_1973 0.55 +0.5 2,281,809 - Shew_1973 0.55 +1.7 2,281,846 - Shew_1973 0.59 +0.7 2,281,878 + Shew_1973 0.62 -0.2 2,281,886 - Shew_1973 0.63 +0.8 2,281,886 - Shew_1973 0.63 +0.1 2,282,180 - +0.4 2,282,383 - -1.7 2,282,383 - -0.4 2,282,582 + Shew_1974 0.26 -0.4 2,282,733 + Shew_1974 0.52 +0.2 2,282,980 + -0.3 2,282,980 + +1.6 2,283,029 + -0.3
Or see this region's nucleotide sequence