Experiment: methylglyoxal 0.001 vol%
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_0208 and Shew_0209 overlap by 13 nucleotides Shew_0209 and Shew_0210 overlap by 4 nucleotides Shew_0210 and Shew_0211 overlap by 4 nucleotides
Shew_0208: Shew_0208 - type IV pilus assembly protein PilM (RefSeq), at 223,714 to 224,793
_0208
Shew_0209: Shew_0209 - fimbrial assembly family protein (RefSeq), at 224,781 to 225,362
_0209
Shew_0210: Shew_0210 - pilus assembly protein, PilO (RefSeq), at 225,359 to 225,982
_0210
Shew_0211: Shew_0211 - pilus assembly protein, PilQ (RefSeq), at 225,979 to 226,494
_0211
Position (kb) 224
225
226 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 223.792 kb on - strand at 223.797 kb on + strand at 223.840 kb on - strand, within Shew_0208 at 223.845 kb on + strand, within Shew_0208 at 223.894 kb on + strand, within Shew_0208 at 223.902 kb on - strand, within Shew_0208 at 223.904 kb on + strand, within Shew_0208 at 223.912 kb on - strand, within Shew_0208 at 224.008 kb on - strand, within Shew_0208 at 224.020 kb on - strand, within Shew_0208 at 224.114 kb on + strand, within Shew_0208 at 224.123 kb on + strand, within Shew_0208 at 224.131 kb on - strand, within Shew_0208 at 224.288 kb on + strand, within Shew_0208 at 224.613 kb on + strand, within Shew_0208 at 224.687 kb on + strand at 224.756 kb on + strand at 224.761 kb on + strand at 224.761 kb on + strand at 224.761 kb on + strand at 224.761 kb on + strand at 224.761 kb on + strand at 224.764 kb on - strand at 224.764 kb on - strand at 224.764 kb on - strand at 224.764 kb on - strand at 224.769 kb on - strand at 224.769 kb on - strand at 224.769 kb on - strand at 224.769 kb on - strand at 224.777 kb on - strand at 225.076 kb on + strand, within Shew_0209 at 225.084 kb on - strand, within Shew_0209 at 225.424 kb on + strand, within Shew_0210 at 225.424 kb on + strand, within Shew_0210 at 225.432 kb on - strand, within Shew_0210 at 225.516 kb on - strand, within Shew_0210 at 225.516 kb on - strand, within Shew_0210 at 225.516 kb on - strand, within Shew_0210 at 225.702 kb on + strand, within Shew_0210 at 225.702 kb on + strand, within Shew_0210 at 225.702 kb on + strand, within Shew_0210 at 225.710 kb on - strand, within Shew_0210 at 225.710 kb on - strand, within Shew_0210 at 225.825 kb on - strand, within Shew_0210 at 225.837 kb on - strand, within Shew_0210 at 226.204 kb on + strand, within Shew_0211 at 226.271 kb on + strand, within Shew_0211 at 226.289 kb on - strand, within Shew_0211
Per-strain Table
Position Strand Gene LocusTag Fraction methylglyoxal 0.001 vol% remove 223,792 - -0.0 223,797 + -0.1 223,840 - Shew_0208 0.12 -0.6 223,845 + Shew_0208 0.12 +0.0 223,894 + Shew_0208 0.17 -0.1 223,902 - Shew_0208 0.17 -0.5 223,904 + Shew_0208 0.18 -3.1 223,912 - Shew_0208 0.18 -0.1 224,008 - Shew_0208 0.27 +0.5 224,020 - Shew_0208 0.28 -0.4 224,114 + Shew_0208 0.37 +0.1 224,123 + Shew_0208 0.38 -0.1 224,131 - Shew_0208 0.39 -0.1 224,288 + Shew_0208 0.53 -0.8 224,613 + Shew_0208 0.83 -0.1 224,687 + +0.4 224,756 + +0.6 224,761 + -0.1 224,761 + -0.2 224,761 + -0.2 224,761 + +1.3 224,761 + -0.2 224,764 - +0.1 224,764 - +0.2 224,764 - -1.0 224,764 - +0.1 224,769 - -0.0 224,769 - -0.0 224,769 - +0.2 224,769 - -1.4 224,777 - +0.1 225,076 + Shew_0209 0.51 +0.0 225,084 - Shew_0209 0.52 +0.4 225,424 + Shew_0210 0.10 +0.1 225,424 + Shew_0210 0.10 +0.4 225,432 - Shew_0210 0.12 +0.1 225,516 - Shew_0210 0.25 +0.1 225,516 - Shew_0210 0.25 -0.2 225,516 - Shew_0210 0.25 -0.3 225,702 + Shew_0210 0.55 +0.2 225,702 + Shew_0210 0.55 -0.4 225,702 + Shew_0210 0.55 -1.9 225,710 - Shew_0210 0.56 +0.1 225,710 - Shew_0210 0.56 +0.1 225,825 - Shew_0210 0.75 +0.2 225,837 - Shew_0210 0.77 -0.0 226,204 + Shew_0211 0.44 -0.2 226,271 + Shew_0211 0.57 -0.0 226,289 - Shew_0211 0.60 -0.5
Or see this region's nucleotide sequence