Experiment: methylglyoxal 0.001 vol%
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_0124 and Shew_0125 are separated by 9 nucleotides Shew_0125 and Shew_0126 are separated by 398 nucleotides Shew_0126 and Shew_0127 overlap by 4 nucleotides
Shew_0124: Shew_0124 - cytochrome-c oxidase (RefSeq), at 133,433 to 135,043
_0124
Shew_0125: Shew_0125 - cytochrome c oxidase, subunit II (RefSeq), at 135,053 to 136,618
_0125
Shew_0126: Shew_0126 - hypothetical protein (RefSeq), at 137,017 to 137,526
_0126
Shew_0127: Shew_0127 - LexA repressor (RefSeq), at 137,523 to 138,140
_0127
Position (kb) 135
136
137 Strain fitness (log2 ratio) -2
-1
0
1
2 at 134.117 kb on - strand, within Shew_0124 at 134.408 kb on - strand, within Shew_0124 at 134.519 kb on - strand, within Shew_0124 at 134.519 kb on - strand, within Shew_0124 at 134.867 kb on - strand, within Shew_0124 at 134.993 kb on - strand at 135.104 kb on - strand at 135.176 kb on - strand at 135.194 kb on - strand at 135.194 kb on - strand at 135.368 kb on + strand, within Shew_0125 at 135.810 kb on + strand, within Shew_0125 at 135.818 kb on - strand, within Shew_0125 at 135.989 kb on + strand, within Shew_0125 at 136.060 kb on + strand, within Shew_0125 at 136.142 kb on + strand, within Shew_0125 at 136.164 kb on + strand, within Shew_0125 at 136.197 kb on + strand, within Shew_0125 at 136.373 kb on - strand, within Shew_0125 at 136.407 kb on + strand, within Shew_0125 at 136.447 kb on + strand, within Shew_0125 at 136.668 kb on - strand at 137.151 kb on + strand, within Shew_0126 at 137.379 kb on + strand, within Shew_0126 at 137.415 kb on + strand, within Shew_0126 at 137.423 kb on - strand, within Shew_0126 at 137.551 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction methylglyoxal 0.001 vol% remove 134,117 - Shew_0124 0.42 +0.4 134,408 - Shew_0124 0.61 -0.1 134,519 - Shew_0124 0.67 -0.1 134,519 - Shew_0124 0.67 +0.1 134,867 - Shew_0124 0.89 -0.3 134,993 - -1.0 135,104 - +0.0 135,176 - +0.2 135,194 - -0.3 135,194 - +0.0 135,368 + Shew_0125 0.20 -0.1 135,810 + Shew_0125 0.48 +1.6 135,818 - Shew_0125 0.49 +0.1 135,989 + Shew_0125 0.60 -0.2 136,060 + Shew_0125 0.64 -0.6 136,142 + Shew_0125 0.70 +2.0 136,164 + Shew_0125 0.71 -0.2 136,197 + Shew_0125 0.73 +2.3 136,373 - Shew_0125 0.84 -0.6 136,407 + Shew_0125 0.86 -0.5 136,447 + Shew_0125 0.89 +0.1 136,668 - -0.6 137,151 + Shew_0126 0.26 -0.2 137,379 + Shew_0126 0.71 +0.2 137,415 + Shew_0126 0.78 -1.8 137,423 - Shew_0126 0.80 +0.3 137,551 + -0.5
Or see this region's nucleotide sequence