Experiment: MoLS4 with L-Glutamine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt escT and invX are separated by 6 nucleotides invX and sctV are separated by 321 nucleotides sctV and DVUA0104.1 overlap by 4 nucleotides DVUA0104.1 and DORF831 are separated by 2 nucleotides DORF831 and DVUA0105 overlap by 4 nucleotides
DVUA0102: escT - type III secretion inner membrane protein (TIGR), at 138,924 to 139,739
escT
DVUA0103: invX - type III secretion protein, HrpO family (TIGR), at 139,746 to 140,006
invX
DVUA0104: sctV - type III secretion inner membrane protein, HrcV family (TIGR), at 140,328 to 142,421
sctV
DVUA0104.1: DVUA0104.1 - no description, at 142,418 to 142,777
DVUA0104.1
DORF831: DORF831 - SEED: FIG00602593: hypothetical protein, at 142,780 to 143,148
DORF831
DVUA0105: DVUA0105 - conserved hypothetical protein (TIGR), at 143,145 to 143,519
DVUA0105
Position (kb)
140
141
142
143 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 139.365 kb on - strand, within escT at 139.374 kb on - strand, within escT at 139.415 kb on + strand, within escT at 139.433 kb on - strand, within escT at 139.467 kb on - strand, within escT at 139.483 kb on + strand, within escT at 139.639 kb on - strand, within escT at 139.683 kb on - strand at 139.699 kb on - strand at 139.699 kb on - strand at 139.801 kb on - strand, within invX at 139.848 kb on - strand, within invX at 139.855 kb on - strand, within invX at 139.860 kb on + strand, within invX at 139.895 kb on + strand, within invX at 139.895 kb on + strand, within invX at 139.895 kb on + strand, within invX at 139.895 kb on + strand, within invX at 139.895 kb on + strand, within invX at 139.903 kb on - strand, within invX at 139.903 kb on - strand, within invX at 139.905 kb on + strand, within invX at 140.073 kb on - strand at 140.168 kb on - strand at 140.213 kb on + strand at 140.224 kb on + strand at 140.244 kb on + strand at 140.269 kb on + strand at 140.270 kb on - strand at 140.333 kb on + strand at 140.409 kb on - strand at 140.413 kb on + strand at 140.446 kb on - strand at 140.589 kb on - strand, within sctV at 140.605 kb on - strand, within sctV at 140.641 kb on - strand, within sctV at 140.648 kb on + strand, within sctV at 140.693 kb on - strand, within sctV at 140.697 kb on + strand, within sctV at 140.697 kb on + strand, within sctV at 140.707 kb on - strand, within sctV at 140.864 kb on + strand, within sctV at 140.864 kb on + strand, within sctV at 140.917 kb on - strand, within sctV at 140.944 kb on - strand, within sctV at 140.978 kb on - strand, within sctV at 141.111 kb on + strand, within sctV at 141.179 kb on - strand, within sctV at 141.190 kb on + strand, within sctV at 141.198 kb on - strand, within sctV at 141.224 kb on + strand, within sctV at 141.224 kb on + strand, within sctV at 141.237 kb on + strand, within sctV at 141.247 kb on + strand, within sctV at 141.266 kb on - strand, within sctV at 141.294 kb on + strand, within sctV at 141.315 kb on + strand, within sctV at 141.339 kb on + strand, within sctV at 141.351 kb on + strand, within sctV at 141.456 kb on + strand, within sctV at 141.501 kb on + strand, within sctV at 141.537 kb on + strand, within sctV at 141.568 kb on + strand, within sctV at 141.568 kb on + strand, within sctV at 141.568 kb on + strand, within sctV at 141.568 kb on + strand, within sctV at 141.576 kb on - strand, within sctV at 141.576 kb on - strand, within sctV at 141.576 kb on - strand, within sctV at 141.576 kb on - strand, within sctV at 141.576 kb on - strand, within sctV at 141.576 kb on - strand, within sctV at 141.576 kb on - strand, within sctV at 141.576 kb on - strand, within sctV at 141.675 kb on + strand, within sctV at 141.686 kb on + strand, within sctV at 141.686 kb on + strand, within sctV at 141.720 kb on + strand, within sctV at 141.740 kb on + strand, within sctV at 141.748 kb on - strand, within sctV at 141.784 kb on + strand, within sctV at 141.784 kb on + strand, within sctV at 141.909 kb on - strand, within sctV at 141.961 kb on + strand, within sctV at 142.040 kb on - strand, within sctV at 142.112 kb on - strand, within sctV at 142.147 kb on - strand, within sctV at 142.147 kb on - strand, within sctV at 142.161 kb on + strand, within sctV at 142.169 kb on - strand, within sctV at 142.169 kb on - strand, within sctV at 142.396 kb on + strand at 142.427 kb on - strand at 142.427 kb on - strand at 142.427 kb on - strand at 142.453 kb on + strand at 142.522 kb on - strand, within DVUA0104.1 at 142.605 kb on - strand, within DVUA0104.1 at 142.627 kb on + strand, within DVUA0104.1 at 142.640 kb on - strand, within DVUA0104.1 at 142.696 kb on + strand, within DVUA0104.1 at 142.731 kb on - strand, within DVUA0104.1 at 142.786 kb on - strand at 143.040 kb on - strand, within DORF831 at 143.275 kb on + strand, within DVUA0105 at 143.345 kb on - strand, within DVUA0105
Per-strain Table
Position Strand Gene LocusTag Fraction MoLS4 with L-Glutamine (N) remove 139,365 - escT DVUA0102 0.54 +0.1 139,374 - escT DVUA0102 0.55 -0.2 139,415 + escT DVUA0102 0.60 +2.0 139,433 - escT DVUA0102 0.62 -0.1 139,467 - escT DVUA0102 0.67 -0.7 139,483 + escT DVUA0102 0.69 -3.1 139,639 - escT DVUA0102 0.88 +1.4 139,683 - -0.4 139,699 - -0.1 139,699 - -0.2 139,801 - invX DVUA0103 0.21 -2.9 139,848 - invX DVUA0103 0.39 -2.8 139,855 - invX DVUA0103 0.42 -3.3 139,860 + invX DVUA0103 0.44 +0.1 139,895 + invX DVUA0103 0.57 +0.8 139,895 + invX DVUA0103 0.57 -0.0 139,895 + invX DVUA0103 0.57 -2.9 139,895 + invX DVUA0103 0.57 -1.7 139,895 + invX DVUA0103 0.57 +0.1 139,903 - invX DVUA0103 0.60 +1.1 139,903 - invX DVUA0103 0.60 -2.7 139,905 + invX DVUA0103 0.61 -0.3 140,073 - -0.2 140,168 - -2.8 140,213 + -0.1 140,224 + +0.3 140,244 + -1.0 140,269 + +0.3 140,270 - -0.8 140,333 + -0.1 140,409 - -1.0 140,413 + +0.3 140,446 - +0.5 140,589 - sctV DVUA0104 0.12 -0.2 140,605 - sctV DVUA0104 0.13 +0.3 140,641 - sctV DVUA0104 0.15 -0.1 140,648 + sctV DVUA0104 0.15 +0.1 140,693 - sctV DVUA0104 0.17 -0.0 140,697 + sctV DVUA0104 0.18 -0.5 140,697 + sctV DVUA0104 0.18 +0.8 140,707 - sctV DVUA0104 0.18 +0.0 140,864 + sctV DVUA0104 0.26 -1.2 140,864 + sctV DVUA0104 0.26 +0.3 140,917 - sctV DVUA0104 0.28 +0.7 140,944 - sctV DVUA0104 0.29 +0.9 140,978 - sctV DVUA0104 0.31 -0.2 141,111 + sctV DVUA0104 0.37 +0.4 141,179 - sctV DVUA0104 0.41 -0.2 141,190 + sctV DVUA0104 0.41 -0.1 141,198 - sctV DVUA0104 0.42 -0.4 141,224 + sctV DVUA0104 0.43 +1.3 141,224 + sctV DVUA0104 0.43 +0.2 141,237 + sctV DVUA0104 0.43 +0.4 141,247 + sctV DVUA0104 0.44 -0.3 141,266 - sctV DVUA0104 0.45 -0.9 141,294 + sctV DVUA0104 0.46 -0.4 141,315 + sctV DVUA0104 0.47 -0.0 141,339 + sctV DVUA0104 0.48 +0.8 141,351 + sctV DVUA0104 0.49 +0.2 141,456 + sctV DVUA0104 0.54 -0.3 141,501 + sctV DVUA0104 0.56 +0.0 141,537 + sctV DVUA0104 0.58 -0.1 141,568 + sctV DVUA0104 0.59 -0.1 141,568 + sctV DVUA0104 0.59 -1.9 141,568 + sctV DVUA0104 0.59 -0.3 141,568 + sctV DVUA0104 0.59 +0.4 141,576 - sctV DVUA0104 0.60 -0.0 141,576 - sctV DVUA0104 0.60 +0.2 141,576 - sctV DVUA0104 0.60 +0.7 141,576 - sctV DVUA0104 0.60 -0.0 141,576 - sctV DVUA0104 0.60 -0.3 141,576 - sctV DVUA0104 0.60 +0.2 141,576 - sctV DVUA0104 0.60 +0.4 141,576 - sctV DVUA0104 0.60 -4.5 141,675 + sctV DVUA0104 0.64 +0.9 141,686 + sctV DVUA0104 0.65 -0.0 141,686 + sctV DVUA0104 0.65 +0.3 141,720 + sctV DVUA0104 0.66 -2.0 141,740 + sctV DVUA0104 0.67 +0.1 141,748 - sctV DVUA0104 0.68 -1.6 141,784 + sctV DVUA0104 0.70 -4.5 141,784 + sctV DVUA0104 0.70 -0.7 141,909 - sctV DVUA0104 0.76 +0.2 141,961 + sctV DVUA0104 0.78 -0.4 142,040 - sctV DVUA0104 0.82 -0.3 142,112 - sctV DVUA0104 0.85 +1.2 142,147 - sctV DVUA0104 0.87 -0.5 142,147 - sctV DVUA0104 0.87 +0.2 142,161 + sctV DVUA0104 0.88 +0.3 142,169 - sctV DVUA0104 0.88 +0.0 142,169 - sctV DVUA0104 0.88 +0.1 142,396 + -0.0 142,427 - +0.5 142,427 - +0.8 142,427 - -0.5 142,453 + +1.1 142,522 - DVUA0104.1 0.29 +0.4 142,605 - DVUA0104.1 0.52 -0.0 142,627 + DVUA0104.1 0.58 -0.2 142,640 - DVUA0104.1 0.62 +0.1 142,696 + DVUA0104.1 0.77 -1.3 142,731 - DVUA0104.1 0.87 +0.1 142,786 - +0.2 143,040 - DORF831 0.70 -0.2 143,275 + DVUA0105 0.35 -2.1 143,345 - DVUA0105 0.53 -4.0
Or see this region's nucleotide sequence