Experiment: MoLS4 with L-Glutamine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt DVU0281 and mutY are separated by 239 nucleotides mutY and DVU0283 are separated by 334 nucleotides
DVU0281: DVU0281 - exopolysaccharide biosynthesis protein, putative (TIGR), at 325,641 to 327,074
DVU0281
DVU0282: mutY - A/G-specific adenine glycosylase (TIGR), at 327,314 to 328,435
mutY
DVU0283: DVU0283 - AhpF family protein/thioredoxin reductase (TIGR), at 328,770 to 330,509
DVU0283
Position (kb) 327
328
329 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1 at 326.783 kb on + strand, within DVU0281 at 326.796 kb on - strand, within DVU0281 at 326.895 kb on + strand, within DVU0281 at 327.011 kb on + strand at 327.130 kb on - strand at 327.312 kb on + strand at 327.319 kb on + strand at 327.319 kb on + strand at 327.320 kb on - strand at 327.327 kb on - strand at 327.337 kb on - strand at 327.337 kb on - strand at 327.379 kb on + strand at 327.387 kb on - strand at 327.445 kb on + strand, within mutY at 327.453 kb on - strand, within mutY at 327.487 kb on - strand, within mutY at 327.494 kb on + strand, within mutY at 327.499 kb on + strand, within mutY at 327.547 kb on - strand, within mutY at 327.669 kb on - strand, within mutY at 327.722 kb on - strand, within mutY at 327.743 kb on - strand, within mutY at 327.752 kb on + strand, within mutY at 327.757 kb on + strand, within mutY at 327.760 kb on - strand, within mutY at 327.877 kb on - strand, within mutY at 327.908 kb on - strand, within mutY at 327.960 kb on - strand, within mutY at 327.976 kb on + strand, within mutY at 327.978 kb on + strand, within mutY at 328.025 kb on + strand, within mutY at 328.071 kb on - strand, within mutY at 328.078 kb on + strand, within mutY at 328.078 kb on + strand, within mutY at 328.097 kb on - strand, within mutY at 328.099 kb on + strand, within mutY at 328.107 kb on - strand, within mutY at 328.131 kb on - strand, within mutY at 328.167 kb on - strand, within mutY at 328.199 kb on - strand, within mutY at 328.199 kb on - strand, within mutY at 328.199 kb on - strand, within mutY at 328.254 kb on - strand, within mutY at 328.280 kb on + strand, within mutY at 328.283 kb on - strand, within mutY at 328.322 kb on + strand, within mutY at 328.323 kb on - strand at 328.340 kb on - strand at 328.340 kb on - strand at 328.396 kb on - strand at 328.427 kb on + strand at 328.427 kb on + strand at 328.443 kb on + strand at 328.448 kb on + strand at 328.448 kb on + strand at 328.456 kb on - strand at 328.480 kb on + strand at 328.490 kb on - strand at 328.625 kb on + strand at 328.690 kb on - strand at 328.695 kb on - strand at 328.756 kb on + strand at 328.770 kb on - strand at 328.777 kb on + strand at 328.915 kb on - strand at 328.945 kb on - strand, within DVU0283 at 328.971 kb on - strand, within DVU0283 at 328.971 kb on - strand, within DVU0283 at 329.002 kb on - strand, within DVU0283 at 329.022 kb on + strand, within DVU0283 at 329.061 kb on + strand, within DVU0283 at 329.081 kb on + strand, within DVU0283 at 329.089 kb on - strand, within DVU0283 at 329.168 kb on - strand, within DVU0283 at 329.179 kb on - strand, within DVU0283 at 329.188 kb on - strand, within DVU0283 at 329.190 kb on + strand, within DVU0283 at 329.223 kb on + strand, within DVU0283 at 329.288 kb on + strand, within DVU0283 at 329.388 kb on + strand, within DVU0283 at 329.424 kb on + strand, within DVU0283 at 329.429 kb on - strand, within DVU0283
Per-strain Table
Position Strand Gene LocusTag Fraction MoLS4 with L-Glutamine (N) remove 326,783 + DVU0281 0.80 -2.8 326,796 - DVU0281 0.81 -0.1 326,895 + DVU0281 0.87 -2.1 327,011 + -2.4 327,130 - -0.4 327,312 + +0.2 327,319 + -0.3 327,319 + -0.4 327,320 - -0.0 327,327 - -0.2 327,337 - -0.9 327,337 - -4.5 327,379 + -3.0 327,387 - +0.1 327,445 + mutY DVU0282 0.12 -0.6 327,453 - mutY DVU0282 0.12 -3.0 327,487 - mutY DVU0282 0.15 -3.5 327,494 + mutY DVU0282 0.16 -0.1 327,499 + mutY DVU0282 0.16 -0.2 327,547 - mutY DVU0282 0.21 -0.2 327,669 - mutY DVU0282 0.32 +0.2 327,722 - mutY DVU0282 0.36 -0.5 327,743 - mutY DVU0282 0.38 -2.9 327,752 + mutY DVU0282 0.39 -0.5 327,757 + mutY DVU0282 0.39 -2.3 327,760 - mutY DVU0282 0.40 -0.6 327,877 - mutY DVU0282 0.50 -3.3 327,908 - mutY DVU0282 0.53 -4.0 327,960 - mutY DVU0282 0.58 -0.5 327,976 + mutY DVU0282 0.59 +0.4 327,978 + mutY DVU0282 0.59 +0.0 328,025 + mutY DVU0282 0.63 -0.1 328,071 - mutY DVU0282 0.67 -4.2 328,078 + mutY DVU0282 0.68 -0.1 328,078 + mutY DVU0282 0.68 -0.6 328,097 - mutY DVU0282 0.70 -3.2 328,099 + mutY DVU0282 0.70 +0.7 328,107 - mutY DVU0282 0.71 -1.4 328,131 - mutY DVU0282 0.73 -0.4 328,167 - mutY DVU0282 0.76 +0.1 328,199 - mutY DVU0282 0.79 -0.1 328,199 - mutY DVU0282 0.79 -0.5 328,199 - mutY DVU0282 0.79 -1.4 328,254 - mutY DVU0282 0.84 -1.9 328,280 + mutY DVU0282 0.86 +1.3 328,283 - mutY DVU0282 0.86 -0.1 328,322 + mutY DVU0282 0.90 -4.0 328,323 - -0.3 328,340 - +0.6 328,340 - -0.3 328,396 - +0.5 328,427 + -0.3 328,427 + -0.2 328,443 + -0.6 328,448 + +1.4 328,448 + -1.4 328,456 - -0.7 328,480 + -3.4 328,490 - -0.2 328,625 + +0.2 328,690 - +0.9 328,695 - +0.4 328,756 + +0.3 328,770 - -1.8 328,777 + +0.1 328,915 - -1.9 328,945 - DVU0283 0.10 +0.2 328,971 - DVU0283 0.12 -0.1 328,971 - DVU0283 0.12 +0.0 329,002 - DVU0283 0.13 -0.3 329,022 + DVU0283 0.14 -1.0 329,061 + DVU0283 0.17 -0.2 329,081 + DVU0283 0.18 -0.5 329,089 - DVU0283 0.18 -2.9 329,168 - DVU0283 0.23 +0.2 329,179 - DVU0283 0.24 -3.4 329,188 - DVU0283 0.24 -0.9 329,190 + DVU0283 0.24 -0.2 329,223 + DVU0283 0.26 -0.6 329,288 + DVU0283 0.30 +0.3 329,388 + DVU0283 0.36 +0.5 329,424 + DVU0283 0.38 +0.5 329,429 - DVU0283 0.38 -0.4
Or see this region's nucleotide sequence