Experiment: MoLS4 with L-Glutamine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt DVU0054 and ispH overlap by 4 nucleotides ispH and cheV-1 are separated by 40 nucleotides cheV-1 and DVU0057 are separated by 259 nucleotides DVU0057 and DVU0058 overlap by 4 nucleotides
DVU0054: DVU0054 - dihydrouridine synthase family protein (TIGR), at 60,507 to 61,766
DVU0054
DVU0055: ispH - hydroxymethylbutenyl pyrophosphate reductase (TIGR), at 61,763 to 62,635
ispH
DVU0056: cheV-1 - chemotaxis protein CheV (TIGR), at 62,676 to 63,629
cheV-1
DVU0057: DVU0057 - transcriptional regulator, TetR family (TIGR), at 63,889 to 64,530
DVU0057
DVU0058: DVU0058 - efflux transporter, RND family, MFP subunit (TIGR), at 64,527 to 65,678
DVU0058
Position (kb) 62
63
64 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1 at 61.709 kb on + strand at 61.729 kb on - strand at 62.704 kb on - strand at 62.758 kb on + strand at 62.767 kb on - strand at 62.767 kb on - strand at 62.853 kb on + strand, within cheV-1 at 62.854 kb on - strand, within cheV-1 at 62.854 kb on - strand, within cheV-1 at 62.859 kb on - strand, within cheV-1 at 62.868 kb on + strand, within cheV-1 at 62.876 kb on - strand, within cheV-1 at 62.876 kb on - strand, within cheV-1 at 62.913 kb on - strand, within cheV-1 at 62.928 kb on - strand, within cheV-1 at 62.928 kb on - strand, within cheV-1 at 62.960 kb on + strand, within cheV-1 at 62.989 kb on - strand, within cheV-1 at 63.049 kb on + strand, within cheV-1 at 63.049 kb on + strand, within cheV-1 at 63.057 kb on - strand, within cheV-1 at 63.160 kb on - strand, within cheV-1 at 63.213 kb on + strand, within cheV-1 at 63.226 kb on - strand, within cheV-1 at 63.247 kb on + strand, within cheV-1 at 63.255 kb on - strand, within cheV-1 at 63.276 kb on + strand, within cheV-1 at 63.330 kb on + strand, within cheV-1 at 63.380 kb on + strand, within cheV-1 at 63.439 kb on + strand, within cheV-1 at 63.439 kb on + strand, within cheV-1 at 63.447 kb on - strand, within cheV-1 at 63.447 kb on - strand, within cheV-1 at 63.447 kb on - strand, within cheV-1 at 63.457 kb on - strand, within cheV-1 at 63.475 kb on - strand, within cheV-1 at 63.484 kb on + strand, within cheV-1 at 63.525 kb on - strand, within cheV-1 at 63.537 kb on + strand at 63.538 kb on - strand at 63.545 kb on - strand at 63.579 kb on + strand at 63.741 kb on + strand at 63.824 kb on + strand at 63.824 kb on + strand at 63.831 kb on - strand at 64.007 kb on + strand, within DVU0057 at 64.077 kb on + strand, within DVU0057 at 64.150 kb on + strand, within DVU0057 at 64.229 kb on + strand, within DVU0057 at 64.229 kb on + strand, within DVU0057 at 64.229 kb on + strand, within DVU0057 at 64.299 kb on - strand, within DVU0057 at 64.345 kb on + strand, within DVU0057 at 64.348 kb on - strand, within DVU0057 at 64.414 kb on + strand, within DVU0057 at 64.416 kb on + strand, within DVU0057 at 64.416 kb on + strand, within DVU0057 at 64.421 kb on + strand, within DVU0057 at 64.421 kb on + strand, within DVU0057 at 64.590 kb on - strand at 64.600 kb on - strand at 64.600 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction MoLS4 with L-Glutamine (N) remove 61,709 + -0.5 61,729 - -0.5 62,704 - -0.8 62,758 + +0.8 62,767 - +0.2 62,767 - -0.2 62,853 + cheV-1 DVU0056 0.19 -0.7 62,854 - cheV-1 DVU0056 0.19 -0.5 62,854 - cheV-1 DVU0056 0.19 +0.3 62,859 - cheV-1 DVU0056 0.19 -0.4 62,868 + cheV-1 DVU0056 0.20 -0.5 62,876 - cheV-1 DVU0056 0.21 -0.0 62,876 - cheV-1 DVU0056 0.21 -0.6 62,913 - cheV-1 DVU0056 0.25 -0.6 62,928 - cheV-1 DVU0056 0.26 -2.6 62,928 - cheV-1 DVU0056 0.26 -1.4 62,960 + cheV-1 DVU0056 0.30 -3.3 62,989 - cheV-1 DVU0056 0.33 -0.1 63,049 + cheV-1 DVU0056 0.39 -4.1 63,049 + cheV-1 DVU0056 0.39 -0.4 63,057 - cheV-1 DVU0056 0.40 -0.8 63,160 - cheV-1 DVU0056 0.51 -0.1 63,213 + cheV-1 DVU0056 0.56 -0.5 63,226 - cheV-1 DVU0056 0.58 +0.9 63,247 + cheV-1 DVU0056 0.60 +0.1 63,255 - cheV-1 DVU0056 0.61 -3.8 63,276 + cheV-1 DVU0056 0.63 -1.0 63,330 + cheV-1 DVU0056 0.69 -0.1 63,380 + cheV-1 DVU0056 0.74 -0.6 63,439 + cheV-1 DVU0056 0.80 -0.6 63,439 + cheV-1 DVU0056 0.80 -3.7 63,447 - cheV-1 DVU0056 0.81 +0.2 63,447 - cheV-1 DVU0056 0.81 +0.0 63,447 - cheV-1 DVU0056 0.81 -0.6 63,457 - cheV-1 DVU0056 0.82 -0.1 63,475 - cheV-1 DVU0056 0.84 +0.3 63,484 + cheV-1 DVU0056 0.85 +0.4 63,525 - cheV-1 DVU0056 0.89 -1.6 63,537 + +0.2 63,538 - +0.0 63,545 - -0.1 63,579 + -3.6 63,741 + -0.1 63,824 + -1.4 63,824 + -2.7 63,831 - -0.5 64,007 + DVU0057 0.18 -0.8 64,077 + DVU0057 0.29 +0.4 64,150 + DVU0057 0.41 +0.2 64,229 + DVU0057 0.53 -2.6 64,229 + DVU0057 0.53 -0.4 64,229 + DVU0057 0.53 -1.1 64,299 - DVU0057 0.64 -0.7 64,345 + DVU0057 0.71 -0.0 64,348 - DVU0057 0.71 -0.2 64,414 + DVU0057 0.82 +0.0 64,416 + DVU0057 0.82 -0.2 64,416 + DVU0057 0.82 -2.6 64,421 + DVU0057 0.83 +0.1 64,421 + DVU0057 0.83 -0.1 64,590 - +0.3 64,600 - +0.4 64,600 - +0.7
Or see this region's nucleotide sequence