Experiment: MoLS4 with L-Glutamine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt nhaC-2 and DVU3109 are separated by 193 nucleotides DVU3109 and DVU3110 are separated by 39 nucleotides DVU3110 and DVU3111 are separated by 246 nucleotides
DVU3108: nhaC-2 - Na+/H+ antiporter NhaC (TIGR), at 3,256,819 to 3,258,246
nhaC-2
DVU3109: DVU3109 - iron-sulfur cluster-binding protein (TIGR), at 3,258,440 to 3,258,640
DVU3109
DVU3110: DVU3110 - L-aspartate oxidase, putative (TIGR), at 3,258,680 to 3,260,401
DVU3110
DVU3111: DVU3111 - transcriptional regulator, Crp/Fnr family (TIGR), at 3,260,648 to 3,261,457
DVU3111
Position (kb)
3258
3259
3260
3261 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1
2 at 3257.706 kb on + strand, within nhaC-2 at 3257.726 kb on + strand, within nhaC-2 at 3257.760 kb on + strand, within nhaC-2 at 3257.815 kb on - strand, within nhaC-2 at 3257.832 kb on + strand, within nhaC-2 at 3257.878 kb on + strand, within nhaC-2 at 3257.936 kb on - strand, within nhaC-2 at 3258.038 kb on + strand, within nhaC-2 at 3258.162 kb on + strand at 3258.165 kb on - strand at 3258.170 kb on - strand at 3258.196 kb on + strand at 3258.196 kb on + strand at 3258.204 kb on - strand at 3258.222 kb on + strand at 3258.326 kb on - strand at 3258.398 kb on + strand at 3258.407 kb on - strand at 3258.411 kb on + strand at 3258.419 kb on - strand at 3258.429 kb on - strand at 3258.454 kb on + strand at 3258.492 kb on + strand, within DVU3109 at 3258.531 kb on + strand, within DVU3109 at 3258.606 kb on - strand, within DVU3109 at 3258.626 kb on - strand at 3258.678 kb on + strand at 3258.773 kb on - strand at 3258.945 kb on + strand, within DVU3110 at 3259.061 kb on - strand, within DVU3110 at 3259.066 kb on - strand, within DVU3110 at 3259.156 kb on - strand, within DVU3110 at 3259.178 kb on + strand, within DVU3110 at 3259.199 kb on + strand, within DVU3110 at 3259.347 kb on - strand, within DVU3110 at 3259.350 kb on + strand, within DVU3110 at 3259.430 kb on + strand, within DVU3110 at 3259.455 kb on + strand, within DVU3110 at 3259.463 kb on - strand, within DVU3110 at 3259.463 kb on - strand, within DVU3110 at 3259.483 kb on + strand, within DVU3110 at 3259.530 kb on + strand, within DVU3110 at 3259.567 kb on + strand, within DVU3110 at 3259.575 kb on - strand, within DVU3110 at 3259.612 kb on + strand, within DVU3110 at 3259.620 kb on - strand, within DVU3110 at 3259.674 kb on + strand, within DVU3110 at 3259.703 kb on + strand, within DVU3110 at 3259.729 kb on - strand, within DVU3110 at 3259.756 kb on - strand, within DVU3110 at 3259.780 kb on - strand, within DVU3110 at 3259.790 kb on - strand, within DVU3110 at 3259.790 kb on - strand, within DVU3110 at 3259.858 kb on - strand, within DVU3110 at 3259.884 kb on - strand, within DVU3110 at 3259.985 kb on - strand, within DVU3110 at 3260.039 kb on + strand, within DVU3110 at 3260.083 kb on + strand, within DVU3110 at 3260.183 kb on + strand, within DVU3110 at 3260.184 kb on - strand, within DVU3110 at 3260.209 kb on + strand, within DVU3110 at 3260.227 kb on - strand, within DVU3110 at 3260.251 kb on - strand at 3260.268 kb on + strand at 3260.308 kb on + strand at 3260.316 kb on - strand at 3260.378 kb on + strand at 3260.386 kb on - strand at 3260.457 kb on + strand at 3260.465 kb on - strand at 3260.472 kb on + strand at 3260.614 kb on - strand at 3260.614 kb on - strand at 3260.620 kb on + strand at 3260.628 kb on - strand at 3260.695 kb on + strand at 3260.722 kb on + strand at 3260.732 kb on + strand, within DVU3111 at 3260.805 kb on - strand, within DVU3111 at 3260.950 kb on - strand, within DVU3111 at 3261.022 kb on - strand, within DVU3111 at 3261.075 kb on - strand, within DVU3111 at 3261.207 kb on - strand, within DVU3111 at 3261.212 kb on - strand, within DVU3111 at 3261.279 kb on - strand, within DVU3111 at 3261.313 kb on - strand, within DVU3111 at 3261.377 kb on + strand at 3261.390 kb on - strand at 3261.397 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction MoLS4 with L-Glutamine (N) remove 3,257,706 + nhaC-2 DVU3108 0.62 +0.1 3,257,726 + nhaC-2 DVU3108 0.64 -0.2 3,257,760 + nhaC-2 DVU3108 0.66 -0.5 3,257,815 - nhaC-2 DVU3108 0.70 -0.0 3,257,832 + nhaC-2 DVU3108 0.71 -0.4 3,257,878 + nhaC-2 DVU3108 0.74 +0.7 3,257,936 - nhaC-2 DVU3108 0.78 +0.7 3,258,038 + nhaC-2 DVU3108 0.85 -0.1 3,258,162 + +0.0 3,258,165 - -0.3 3,258,170 - +0.1 3,258,196 + +0.1 3,258,196 + -5.1 3,258,204 - -0.8 3,258,222 + +0.1 3,258,326 - -0.5 3,258,398 + -3.1 3,258,407 - -0.2 3,258,411 + -0.2 3,258,419 - -1.3 3,258,429 - -0.2 3,258,454 + -0.4 3,258,492 + DVU3109 0.26 -1.0 3,258,531 + DVU3109 0.45 -4.0 3,258,606 - DVU3109 0.83 -0.2 3,258,626 - -0.4 3,258,678 + -0.6 3,258,773 - -0.1 3,258,945 + DVU3110 0.15 -0.6 3,259,061 - DVU3110 0.22 -0.2 3,259,066 - DVU3110 0.22 +0.4 3,259,156 - DVU3110 0.28 +1.0 3,259,178 + DVU3110 0.29 -0.5 3,259,199 + DVU3110 0.30 -0.5 3,259,347 - DVU3110 0.39 -0.4 3,259,350 + DVU3110 0.39 +0.0 3,259,430 + DVU3110 0.44 +0.7 3,259,455 + DVU3110 0.45 -3.6 3,259,463 - DVU3110 0.45 +1.3 3,259,463 - DVU3110 0.45 -0.3 3,259,483 + DVU3110 0.47 -0.0 3,259,530 + DVU3110 0.49 -0.5 3,259,567 + DVU3110 0.52 +0.7 3,259,575 - DVU3110 0.52 +1.9 3,259,612 + DVU3110 0.54 +0.5 3,259,620 - DVU3110 0.55 -1.1 3,259,674 + DVU3110 0.58 -0.1 3,259,703 + DVU3110 0.59 +0.2 3,259,729 - DVU3110 0.61 -0.7 3,259,756 - DVU3110 0.62 -0.1 3,259,780 - DVU3110 0.64 +0.9 3,259,790 - DVU3110 0.64 -3.4 3,259,790 - DVU3110 0.64 -0.2 3,259,858 - DVU3110 0.68 -0.5 3,259,884 - DVU3110 0.70 +0.1 3,259,985 - DVU3110 0.76 -1.8 3,260,039 + DVU3110 0.79 -0.5 3,260,083 + DVU3110 0.81 -0.0 3,260,183 + DVU3110 0.87 -3.4 3,260,184 - DVU3110 0.87 -0.3 3,260,209 + DVU3110 0.89 +0.0 3,260,227 - DVU3110 0.90 -0.5 3,260,251 - +0.4 3,260,268 + -0.6 3,260,308 + -0.2 3,260,316 - +0.0 3,260,378 + +1.0 3,260,386 - -1.0 3,260,457 + +0.2 3,260,465 - +0.0 3,260,472 + -3.0 3,260,614 - -0.0 3,260,614 - +0.2 3,260,620 + +0.1 3,260,628 - +0.2 3,260,695 + +0.4 3,260,722 + +0.1 3,260,732 + DVU3111 0.10 -0.2 3,260,805 - DVU3111 0.19 -0.2 3,260,950 - DVU3111 0.37 +0.1 3,261,022 - DVU3111 0.46 +0.3 3,261,075 - DVU3111 0.53 +0.4 3,261,207 - DVU3111 0.69 -0.3 3,261,212 - DVU3111 0.70 -3.1 3,261,279 - DVU3111 0.78 -3.7 3,261,313 - DVU3111 0.82 -0.2 3,261,377 + -4.1 3,261,390 - +0.4 3,261,397 + +1.1
Or see this region's nucleotide sequence