Experiment: MoLS4 with L-Glutamine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt leuB and pspC are separated by 608 nucleotides pspC and DVU2987 overlap by 4 nucleotides DVU2987 and pspA are separated by 78 nucleotides
DVU2985: leuB - 3-isopropylmalate dehydrogenase (TIGR), at 3,092,192 to 3,093,265
leuB
DVU2986: pspC - phage shock protein C (TIGR), at 3,093,874 to 3,094,296
pspC
DVU2987: DVU2987 - hypothetical protein (TIGR), at 3,094,293 to 3,094,592
DVU2987
DVU2988: pspA - phage shock protein A (TIGR), at 3,094,671 to 3,095,336
pspA
Position (kb)
3093
3094
3095 Strain fitness (log2 ratio)
-7
-6
-5
-4
-3
-2
-1
0
1 at 3092.897 kb on - strand, within leuB at 3092.905 kb on - strand, within leuB at 3092.931 kb on - strand, within leuB at 3092.990 kb on + strand, within leuB at 3093.019 kb on + strand, within leuB at 3093.061 kb on + strand, within leuB at 3093.061 kb on + strand, within leuB at 3093.061 kb on + strand, within leuB at 3093.061 kb on + strand, within leuB at 3093.061 kb on + strand, within leuB at 3093.061 kb on + strand, within leuB at 3093.067 kb on + strand, within leuB at 3093.069 kb on - strand, within leuB at 3093.069 kb on - strand, within leuB at 3093.069 kb on - strand, within leuB at 3093.069 kb on - strand, within leuB at 3093.069 kb on - strand, within leuB at 3093.075 kb on - strand, within leuB at 3093.117 kb on + strand, within leuB at 3093.209 kb on + strand at 3093.218 kb on + strand at 3093.220 kb on + strand at 3093.226 kb on - strand at 3093.317 kb on + strand at 3093.375 kb on + strand at 3093.419 kb on - strand at 3093.439 kb on + strand at 3093.447 kb on - strand at 3093.449 kb on - strand at 3093.690 kb on - strand at 3093.979 kb on - strand, within pspC at 3093.986 kb on - strand, within pspC at 3093.987 kb on + strand, within pspC at 3094.056 kb on - strand, within pspC at 3094.064 kb on + strand, within pspC at 3094.141 kb on - strand, within pspC at 3094.199 kb on + strand, within pspC at 3094.207 kb on - strand, within pspC at 3094.207 kb on - strand, within pspC at 3094.207 kb on - strand, within pspC at 3094.207 kb on - strand, within pspC at 3094.233 kb on - strand, within pspC at 3094.307 kb on + strand at 3094.376 kb on + strand, within DVU2987 at 3094.376 kb on + strand, within DVU2987 at 3094.384 kb on - strand, within DVU2987 at 3094.388 kb on + strand, within DVU2987 at 3094.388 kb on + strand, within DVU2987 at 3094.388 kb on + strand, within DVU2987 at 3094.393 kb on + strand, within DVU2987 at 3094.396 kb on - strand, within DVU2987 at 3094.516 kb on + strand, within DVU2987 at 3094.524 kb on - strand, within DVU2987 at 3094.534 kb on - strand, within DVU2987 at 3094.543 kb on - strand, within DVU2987 at 3094.543 kb on - strand, within DVU2987 at 3094.556 kb on + strand, within DVU2987 at 3094.556 kb on + strand, within DVU2987 at 3094.564 kb on - strand at 3094.570 kb on + strand at 3094.578 kb on - strand at 3094.631 kb on - strand at 3094.645 kb on - strand at 3094.645 kb on - strand at 3094.645 kb on - strand at 3094.645 kb on - strand at 3094.677 kb on + strand at 3094.899 kb on + strand, within pspA at 3094.907 kb on - strand, within pspA at 3094.958 kb on + strand, within pspA at 3094.966 kb on - strand, within pspA at 3094.976 kb on + strand, within pspA at 3094.977 kb on - strand, within pspA at 3095.009 kb on + strand, within pspA at 3095.037 kb on - strand, within pspA at 3095.147 kb on - strand, within pspA at 3095.157 kb on - strand, within pspA at 3095.178 kb on - strand, within pspA at 3095.186 kb on - strand, within pspA at 3095.190 kb on - strand, within pspA
Per-strain Table
Position Strand Gene LocusTag Fraction MoLS4 with L-Glutamine (N) remove 3,092,897 - leuB DVU2985 0.66 -4.9 3,092,905 - leuB DVU2985 0.66 -2.0 3,092,931 - leuB DVU2985 0.69 -5.0 3,092,990 + leuB DVU2985 0.74 -5.4 3,093,019 + leuB DVU2985 0.77 -1.7 3,093,061 + leuB DVU2985 0.81 -2.1 3,093,061 + leuB DVU2985 0.81 -4.6 3,093,061 + leuB DVU2985 0.81 -4.6 3,093,061 + leuB DVU2985 0.81 -4.0 3,093,061 + leuB DVU2985 0.81 -5.6 3,093,061 + leuB DVU2985 0.81 -3.9 3,093,067 + leuB DVU2985 0.81 -3.4 3,093,069 - leuB DVU2985 0.82 -5.7 3,093,069 - leuB DVU2985 0.82 -4.7 3,093,069 - leuB DVU2985 0.82 -3.8 3,093,069 - leuB DVU2985 0.82 -3.3 3,093,069 - leuB DVU2985 0.82 -1.5 3,093,075 - leuB DVU2985 0.82 -4.3 3,093,117 + leuB DVU2985 0.86 -4.7 3,093,209 + -0.9 3,093,218 + -4.4 3,093,220 + -5.2 3,093,226 - -6.9 3,093,317 + -0.2 3,093,375 + -0.8 3,093,419 - -0.0 3,093,439 + +0.1 3,093,447 - -0.3 3,093,449 - -2.6 3,093,690 - -0.4 3,093,979 - pspC DVU2986 0.25 +0.1 3,093,986 - pspC DVU2986 0.26 +0.2 3,093,987 + pspC DVU2986 0.27 +1.5 3,094,056 - pspC DVU2986 0.43 +0.9 3,094,064 + pspC DVU2986 0.45 -0.1 3,094,141 - pspC DVU2986 0.63 -0.4 3,094,199 + pspC DVU2986 0.77 -2.5 3,094,207 - pspC DVU2986 0.79 -0.0 3,094,207 - pspC DVU2986 0.79 +0.3 3,094,207 - pspC DVU2986 0.79 -0.2 3,094,207 - pspC DVU2986 0.79 -0.1 3,094,233 - pspC DVU2986 0.85 +1.2 3,094,307 + +0.7 3,094,376 + DVU2987 0.28 -0.5 3,094,376 + DVU2987 0.28 -1.1 3,094,384 - DVU2987 0.30 -0.1 3,094,388 + DVU2987 0.32 +0.6 3,094,388 + DVU2987 0.32 +0.5 3,094,388 + DVU2987 0.32 +0.4 3,094,393 + DVU2987 0.33 -3.6 3,094,396 - DVU2987 0.34 -0.3 3,094,516 + DVU2987 0.74 -3.0 3,094,524 - DVU2987 0.77 -2.3 3,094,534 - DVU2987 0.80 -0.1 3,094,543 - DVU2987 0.83 -0.1 3,094,543 - DVU2987 0.83 -3.0 3,094,556 + DVU2987 0.88 -0.1 3,094,556 + DVU2987 0.88 -0.0 3,094,564 - +0.5 3,094,570 + +0.2 3,094,578 - +1.2 3,094,631 - -0.1 3,094,645 - +0.2 3,094,645 - -0.6 3,094,645 - +0.3 3,094,645 - +0.0 3,094,677 + -0.2 3,094,899 + pspA DVU2988 0.34 -0.8 3,094,907 - pspA DVU2988 0.35 +0.1 3,094,958 + pspA DVU2988 0.43 +0.2 3,094,966 - pspA DVU2988 0.44 +0.1 3,094,976 + pspA DVU2988 0.46 +0.2 3,094,977 - pspA DVU2988 0.46 +0.0 3,095,009 + pspA DVU2988 0.51 -0.6 3,095,037 - pspA DVU2988 0.55 +0.8 3,095,147 - pspA DVU2988 0.71 -0.1 3,095,157 - pspA DVU2988 0.73 -0.3 3,095,178 - pspA DVU2988 0.76 +0.6 3,095,186 - pspA DVU2988 0.77 +0.2 3,095,190 - pspA DVU2988 0.78 -4.0
Or see this region's nucleotide sequence