Experiment: MoLS4 with L-Glutamine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt DVU2953 and tRNA-Gln are separated by 84 nucleotides tRNA-Gln and tRNA-Glu are separated by 17 nucleotides tRNA-Glu and DVU2954 are separated by 139 nucleotides DVU2954 and flrA are separated by 419 nucleotides
DVU2953: DVU2953 - transcriptional regulator, GntR family (TIGR), at 3,060,107 to 3,061,669
DVU2953
DVU_tRNA-Gln-2: tRNA-Gln - tRNA with anticodon CTG for Gln (VIMSS-AUTO), at 3,061,754 to 3,061,828
tRNA-Gln
DVU_tRNA-Glu-3: tRNA-Glu - tRNA with anticodon TTC for Glu (VIMSS-AUTO), at 3,061,846 to 3,061,923
tRNA-Glu
DVU2954: DVU2954 - GGDEF domain protein (TIGR), at 3,062,063 to 3,063,742
DVU2954
DVU2956: flrA - sigma-54 dependent transcriptional regulator (TIGR), at 3,064,162 to 3,065,199
flrA
Position (kb)
3062
3063
3064 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 3061.080 kb on + strand, within DVU2953 at 3061.126 kb on - strand, within DVU2953 at 3061.136 kb on - strand, within DVU2953 at 3061.136 kb on - strand, within DVU2953 at 3061.158 kb on - strand, within DVU2953 at 3061.226 kb on - strand, within DVU2953 at 3061.243 kb on + strand, within DVU2953 at 3061.373 kb on + strand, within DVU2953 at 3061.373 kb on - strand, within DVU2953 at 3061.381 kb on - strand, within DVU2953 at 3061.478 kb on + strand, within DVU2953 at 3061.486 kb on - strand, within DVU2953 at 3061.526 kb on + strand at 3061.565 kb on - strand at 3061.628 kb on - strand at 3061.652 kb on + strand at 3061.652 kb on + strand at 3061.934 kb on + strand at 3061.977 kb on + strand at 3062.015 kb on + strand at 3062.019 kb on + strand at 3062.225 kb on + strand at 3062.276 kb on + strand, within DVU2954 at 3062.276 kb on + strand, within DVU2954 at 3062.284 kb on - strand, within DVU2954 at 3062.299 kb on + strand, within DVU2954 at 3062.309 kb on + strand, within DVU2954 at 3062.309 kb on + strand, within DVU2954 at 3062.309 kb on + strand, within DVU2954 at 3062.309 kb on + strand, within DVU2954 at 3062.317 kb on - strand, within DVU2954 at 3062.365 kb on + strand, within DVU2954 at 3062.365 kb on + strand, within DVU2954 at 3062.715 kb on + strand, within DVU2954 at 3062.715 kb on + strand, within DVU2954 at 3062.723 kb on - strand, within DVU2954 at 3062.834 kb on + strand, within DVU2954 at 3062.842 kb on - strand, within DVU2954 at 3062.865 kb on - strand, within DVU2954 at 3062.987 kb on + strand, within DVU2954 at 3062.995 kb on - strand, within DVU2954 at 3063.038 kb on + strand, within DVU2954 at 3063.071 kb on + strand, within DVU2954 at 3063.079 kb on - strand, within DVU2954 at 3063.084 kb on + strand, within DVU2954 at 3063.084 kb on - strand, within DVU2954 at 3063.126 kb on - strand, within DVU2954 at 3063.144 kb on + strand, within DVU2954 at 3063.144 kb on + strand, within DVU2954 at 3063.184 kb on - strand, within DVU2954 at 3063.239 kb on + strand, within DVU2954 at 3063.269 kb on + strand, within DVU2954 at 3063.342 kb on + strand, within DVU2954 at 3063.373 kb on + strand, within DVU2954 at 3063.432 kb on + strand, within DVU2954 at 3063.442 kb on - strand, within DVU2954 at 3063.442 kb on - strand, within DVU2954 at 3063.587 kb on - strand at 3063.620 kb on + strand at 3063.628 kb on - strand at 3063.652 kb on - strand at 3063.665 kb on + strand at 3063.665 kb on + strand at 3063.685 kb on + strand at 3063.693 kb on - strand at 3063.714 kb on + strand at 3063.714 kb on + strand at 3063.714 kb on + strand at 3063.714 kb on + strand at 3063.722 kb on - strand at 3063.722 kb on - strand at 3063.722 kb on - strand at 3063.722 kb on - strand at 3063.722 kb on - strand at 3063.745 kb on - strand at 3063.985 kb on + strand at 3063.985 kb on + strand at 3063.987 kb on + strand at 3064.011 kb on + strand at 3064.019 kb on - strand at 3064.200 kb on + strand at 3064.209 kb on + strand at 3064.209 kb on + strand at 3064.265 kb on + strand at 3064.715 kb on + strand, within flrA
Per-strain Table
Position Strand Gene LocusTag Fraction MoLS4 with L-Glutamine (N) remove 3,061,080 + DVU2953 0.62 +0.3 3,061,126 - DVU2953 0.65 -0.2 3,061,136 - DVU2953 0.66 -0.0 3,061,136 - DVU2953 0.66 +1.7 3,061,158 - DVU2953 0.67 +0.2 3,061,226 - DVU2953 0.72 -0.4 3,061,243 + DVU2953 0.73 -0.3 3,061,373 + DVU2953 0.81 +0.2 3,061,373 - DVU2953 0.81 -1.0 3,061,381 - DVU2953 0.82 -2.7 3,061,478 + DVU2953 0.88 -1.1 3,061,486 - DVU2953 0.88 +0.4 3,061,526 + +0.5 3,061,565 - -0.4 3,061,628 - +0.7 3,061,652 + -0.2 3,061,652 + +0.0 3,061,934 + -0.3 3,061,977 + -1.1 3,062,015 + +0.1 3,062,019 + +0.2 3,062,225 + -0.0 3,062,276 + DVU2954 0.13 +0.2 3,062,276 + DVU2954 0.13 +0.8 3,062,284 - DVU2954 0.13 -0.4 3,062,299 + DVU2954 0.14 -0.1 3,062,309 + DVU2954 0.15 +0.1 3,062,309 + DVU2954 0.15 -0.3 3,062,309 + DVU2954 0.15 +0.6 3,062,309 + DVU2954 0.15 +0.5 3,062,317 - DVU2954 0.15 -0.0 3,062,365 + DVU2954 0.18 +1.9 3,062,365 + DVU2954 0.18 +0.4 3,062,715 + DVU2954 0.39 -0.2 3,062,715 + DVU2954 0.39 -0.4 3,062,723 - DVU2954 0.39 -1.6 3,062,834 + DVU2954 0.46 -0.0 3,062,842 - DVU2954 0.46 -0.1 3,062,865 - DVU2954 0.48 -4.0 3,062,987 + DVU2954 0.55 +0.9 3,062,995 - DVU2954 0.55 +0.0 3,063,038 + DVU2954 0.58 +0.5 3,063,071 + DVU2954 0.60 +0.9 3,063,079 - DVU2954 0.60 +0.6 3,063,084 + DVU2954 0.61 +0.3 3,063,084 - DVU2954 0.61 -0.1 3,063,126 - DVU2954 0.63 -0.3 3,063,144 + DVU2954 0.64 -0.0 3,063,144 + DVU2954 0.64 +0.2 3,063,184 - DVU2954 0.67 +0.1 3,063,239 + DVU2954 0.70 -0.5 3,063,269 + DVU2954 0.72 -0.2 3,063,342 + DVU2954 0.76 -0.9 3,063,373 + DVU2954 0.78 -0.1 3,063,432 + DVU2954 0.81 +0.1 3,063,442 - DVU2954 0.82 -0.1 3,063,442 - DVU2954 0.82 -0.1 3,063,587 - -0.1 3,063,620 + -0.1 3,063,628 - -0.1 3,063,652 - -2.9 3,063,665 + +0.8 3,063,665 + -0.4 3,063,685 + -0.3 3,063,693 - -0.1 3,063,714 + -0.0 3,063,714 + -0.2 3,063,714 + -3.1 3,063,714 + -0.6 3,063,722 - -0.6 3,063,722 - -3.4 3,063,722 - +0.7 3,063,722 - -0.2 3,063,722 - -2.7 3,063,745 - -0.3 3,063,985 + -0.6 3,063,985 + -0.7 3,063,987 + -0.3 3,064,011 + +0.3 3,064,019 - -0.3 3,064,200 + -1.5 3,064,209 + -1.5 3,064,209 + -0.8 3,064,265 + -0.6 3,064,715 + flrA DVU2956 0.53 +0.4
Or see this region's nucleotide sequence