Experiment: MoLS4 with L-Glutamine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt panB and DVU2447 are separated by 118 nucleotides DVU2447 and panC are separated by 172 nucleotides
DVU2446: panB - 3-methyl-2-oxobutanoate hydroxymethyltransferase (TIGR), at 2,550,513 to 2,551,436
panB
DVU2447: DVU2447 - hypothetical protein (TIGR), at 2,551,555 to 2,552,130
DVU2447
DVU2448: panC - pantoate--beta-alanine ligase (TIGR), at 2,552,303 to 2,553,154
panC
Position (kb)
2551
2552
2553 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 2550.561 kb on + strand at 2550.562 kb on + strand at 2550.570 kb on - strand at 2550.570 kb on - strand at 2550.571 kb on - strand at 2550.604 kb on - strand at 2550.630 kb on + strand, within panB at 2550.638 kb on - strand, within panB at 2550.782 kb on - strand, within panB at 2550.804 kb on - strand, within panB at 2550.809 kb on - strand, within panB at 2550.850 kb on - strand, within panB at 2550.852 kb on + strand, within panB at 2550.854 kb on + strand, within panB at 2550.854 kb on + strand, within panB at 2550.860 kb on - strand, within panB at 2550.862 kb on - strand, within panB at 2550.862 kb on - strand, within panB at 2550.862 kb on - strand, within panB at 2550.862 kb on - strand, within panB at 2550.940 kb on - strand, within panB at 2551.020 kb on + strand, within panB at 2551.103 kb on + strand, within panB at 2551.219 kb on + strand, within panB at 2551.254 kb on + strand, within panB at 2551.334 kb on - strand, within panB at 2551.533 kb on + strand at 2551.572 kb on + strand at 2551.572 kb on + strand at 2551.572 kb on + strand at 2551.580 kb on - strand at 2551.584 kb on - strand at 2551.730 kb on - strand, within DVU2447 at 2551.732 kb on - strand, within DVU2447 at 2551.782 kb on + strand, within DVU2447 at 2551.813 kb on - strand, within DVU2447 at 2551.875 kb on - strand, within DVU2447 at 2551.921 kb on + strand, within DVU2447 at 2551.929 kb on - strand, within DVU2447 at 2551.983 kb on - strand, within DVU2447 at 2552.012 kb on - strand, within DVU2447 at 2552.051 kb on - strand, within DVU2447 at 2552.077 kb on - strand at 2552.090 kb on + strand at 2552.095 kb on + strand at 2552.150 kb on + strand at 2552.169 kb on + strand at 2552.205 kb on - strand at 2552.207 kb on + strand at 2552.215 kb on - strand at 2552.222 kb on - strand at 2552.222 kb on - strand at 2552.241 kb on - strand at 2552.243 kb on - strand at 2552.243 kb on - strand at 2552.243 kb on - strand at 2552.253 kb on - strand at 2552.408 kb on + strand, within panC at 2552.416 kb on - strand, within panC at 2552.416 kb on - strand, within panC at 2552.416 kb on - strand, within panC at 2552.416 kb on - strand, within panC at 2552.431 kb on + strand, within panC at 2552.434 kb on - strand, within panC at 2552.596 kb on + strand, within panC at 2552.669 kb on + strand, within panC at 2552.674 kb on + strand, within panC at 2552.674 kb on + strand, within panC at 2552.682 kb on - strand, within panC at 2552.682 kb on - strand, within panC at 2552.682 kb on - strand, within panC at 2552.683 kb on + strand, within panC at 2552.691 kb on - strand, within panC at 2552.691 kb on - strand, within panC at 2552.865 kb on + strand, within panC at 2552.873 kb on - strand, within panC at 2552.874 kb on - strand, within panC at 2552.942 kb on - strand, within panC at 2552.952 kb on - strand, within panC at 2553.025 kb on - strand, within panC at 2553.039 kb on - strand, within panC at 2553.051 kb on + strand, within panC at 2553.051 kb on + strand, within panC at 2553.078 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction MoLS4 with L-Glutamine (N) remove 2,550,561 + -0.8 2,550,562 + -0.3 2,550,570 - -0.3 2,550,570 - -0.4 2,550,571 - +0.0 2,550,604 - +0.3 2,550,630 + panB DVU2446 0.13 -1.1 2,550,638 - panB DVU2446 0.14 -0.5 2,550,782 - panB DVU2446 0.29 -0.2 2,550,804 - panB DVU2446 0.31 +0.5 2,550,809 - panB DVU2446 0.32 -0.4 2,550,850 - panB DVU2446 0.36 -0.4 2,550,852 + panB DVU2446 0.37 -0.8 2,550,854 + panB DVU2446 0.37 +0.3 2,550,854 + panB DVU2446 0.37 +0.0 2,550,860 - panB DVU2446 0.38 -0.1 2,550,862 - panB DVU2446 0.38 +0.1 2,550,862 - panB DVU2446 0.38 -0.6 2,550,862 - panB DVU2446 0.38 +0.7 2,550,862 - panB DVU2446 0.38 -2.8 2,550,940 - panB DVU2446 0.46 -0.0 2,551,020 + panB DVU2446 0.55 -0.3 2,551,103 + panB DVU2446 0.64 -0.1 2,551,219 + panB DVU2446 0.76 -0.5 2,551,254 + panB DVU2446 0.80 -0.1 2,551,334 - panB DVU2446 0.89 -0.0 2,551,533 + -0.1 2,551,572 + -0.1 2,551,572 + -2.6 2,551,572 + -0.3 2,551,580 - +0.1 2,551,584 - -2.9 2,551,730 - DVU2447 0.30 -0.4 2,551,732 - DVU2447 0.31 +0.1 2,551,782 + DVU2447 0.39 -3.4 2,551,813 - DVU2447 0.45 -0.4 2,551,875 - DVU2447 0.56 -0.1 2,551,921 + DVU2447 0.64 -0.3 2,551,929 - DVU2447 0.65 -2.6 2,551,983 - DVU2447 0.74 -0.7 2,552,012 - DVU2447 0.79 -3.1 2,552,051 - DVU2447 0.86 -0.3 2,552,077 - -0.5 2,552,090 + -0.8 2,552,095 + -0.1 2,552,150 + -0.1 2,552,169 + +0.3 2,552,205 - -0.5 2,552,207 + -0.3 2,552,215 - -2.1 2,552,222 - -0.5 2,552,222 - -0.3 2,552,241 - -0.5 2,552,243 - -0.6 2,552,243 - -2.1 2,552,243 - +0.2 2,552,253 - -1.8 2,552,408 + panC DVU2448 0.12 -2.1 2,552,416 - panC DVU2448 0.13 -2.1 2,552,416 - panC DVU2448 0.13 -1.9 2,552,416 - panC DVU2448 0.13 -0.7 2,552,416 - panC DVU2448 0.13 -1.0 2,552,431 + panC DVU2448 0.15 -0.2 2,552,434 - panC DVU2448 0.15 -0.6 2,552,596 + panC DVU2448 0.34 -0.2 2,552,669 + panC DVU2448 0.43 +0.2 2,552,674 + panC DVU2448 0.44 -0.5 2,552,674 + panC DVU2448 0.44 +0.2 2,552,682 - panC DVU2448 0.44 -1.5 2,552,682 - panC DVU2448 0.44 +0.4 2,552,682 - panC DVU2448 0.44 -2.5 2,552,683 + panC DVU2448 0.45 -0.3 2,552,691 - panC DVU2448 0.46 -0.5 2,552,691 - panC DVU2448 0.46 +0.1 2,552,865 + panC DVU2448 0.66 -0.8 2,552,873 - panC DVU2448 0.67 -0.7 2,552,874 - panC DVU2448 0.67 +0.2 2,552,942 - panC DVU2448 0.75 -0.7 2,552,952 - panC DVU2448 0.76 -3.2 2,553,025 - panC DVU2448 0.85 -1.5 2,553,039 - panC DVU2448 0.86 -0.0 2,553,051 + panC DVU2448 0.88 -0.3 2,553,051 + panC DVU2448 0.88 -3.3 2,553,078 + -0.5
Or see this region's nucleotide sequence