Experiment: MoLS4 with L-Glutamine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt pflA and DVU2272 are separated by 111 nucleotides DVU2272 and DVU2274 are separated by 294 nucleotides DVU2274 and rocR are separated by 61 nucleotides
DVU2271: pflA - pyruvate formate-lyase activating enzyme, putative (TIGR), at 2,360,039 to 2,360,932
pflA
DVU2272: DVU2272 - formate acetyltransferase, putative (TIGR), at 2,361,044 to 2,363,497
DVU2272
DVU2274: DVU2274 - hypothetical protein (TIGR), at 2,363,792 to 2,363,929
DVU2274
DVU2275: rocR - sigma-54 dependent transcriptional regulator (TIGR), at 2,363,991 to 2,365,445
rocR
Position (kb)
2361
2362
2363
2364 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 2360.076 kb on - strand at 2360.086 kb on - strand at 2360.088 kb on + strand at 2360.096 kb on - strand at 2360.116 kb on - strand at 2360.116 kb on - strand at 2360.143 kb on - strand, within pflA at 2360.166 kb on + strand, within pflA at 2360.181 kb on + strand, within pflA at 2360.181 kb on + strand, within pflA at 2360.181 kb on + strand, within pflA at 2360.233 kb on - strand, within pflA at 2360.293 kb on - strand, within pflA at 2360.336 kb on + strand, within pflA at 2360.407 kb on + strand, within pflA at 2360.416 kb on - strand, within pflA at 2360.487 kb on + strand, within pflA at 2360.487 kb on + strand, within pflA at 2360.495 kb on - strand, within pflA at 2360.495 kb on - strand, within pflA at 2360.595 kb on + strand, within pflA at 2360.603 kb on - strand, within pflA at 2360.613 kb on + strand, within pflA at 2360.613 kb on + strand, within pflA at 2360.621 kb on - strand, within pflA at 2360.666 kb on - strand, within pflA at 2360.666 kb on - strand, within pflA at 2360.666 kb on - strand, within pflA at 2360.766 kb on + strand, within pflA at 2360.766 kb on + strand, within pflA at 2360.766 kb on + strand, within pflA at 2360.766 kb on + strand, within pflA at 2360.766 kb on + strand, within pflA at 2360.774 kb on - strand, within pflA at 2360.774 kb on - strand, within pflA at 2360.827 kb on + strand, within pflA at 2360.837 kb on + strand, within pflA at 2360.970 kb on - strand at 2360.970 kb on - strand at 2360.970 kb on - strand at 2360.980 kb on - strand at 2361.023 kb on - strand at 2361.095 kb on - strand at 2361.095 kb on - strand at 2361.119 kb on - strand at 2361.121 kb on - strand at 2361.156 kb on + strand at 2361.156 kb on + strand at 2361.197 kb on + strand at 2361.234 kb on + strand at 2361.242 kb on - strand at 2361.242 kb on - strand at 2361.288 kb on + strand at 2361.323 kb on - strand, within DVU2272 at 2361.392 kb on - strand, within DVU2272 at 2361.514 kb on + strand, within DVU2272 at 2361.540 kb on + strand, within DVU2272 at 2361.550 kb on - strand, within DVU2272 at 2361.581 kb on + strand, within DVU2272 at 2361.589 kb on - strand, within DVU2272 at 2361.778 kb on - strand, within DVU2272 at 2361.791 kb on + strand, within DVU2272 at 2361.875 kb on - strand, within DVU2272 at 2361.914 kb on + strand, within DVU2272 at 2361.968 kb on + strand, within DVU2272 at 2362.150 kb on + strand, within DVU2272 at 2362.158 kb on - strand, within DVU2272 at 2362.175 kb on - strand, within DVU2272 at 2362.187 kb on + strand, within DVU2272 at 2362.296 kb on + strand, within DVU2272 at 2362.301 kb on + strand, within DVU2272 at 2362.334 kb on - strand, within DVU2272 at 2362.342 kb on + strand, within DVU2272 at 2362.423 kb on - strand, within DVU2272 at 2362.425 kb on + strand, within DVU2272 at 2362.446 kb on - strand, within DVU2272 at 2362.447 kb on - strand, within DVU2272 at 2362.447 kb on - strand, within DVU2272 at 2362.526 kb on + strand, within DVU2272 at 2362.563 kb on + strand, within DVU2272 at 2362.584 kb on + strand, within DVU2272 at 2362.607 kb on + strand, within DVU2272 at 2362.663 kb on - strand, within DVU2272 at 2362.720 kb on + strand, within DVU2272 at 2362.728 kb on - strand, within DVU2272 at 2362.880 kb on - strand, within DVU2272 at 2362.907 kb on + strand, within DVU2272 at 2362.914 kb on + strand, within DVU2272 at 2362.920 kb on - strand, within DVU2272 at 2362.979 kb on + strand, within DVU2272 at 2362.987 kb on - strand, within DVU2272 at 2363.024 kb on - strand, within DVU2272 at 2363.050 kb on - strand, within DVU2272 at 2363.125 kb on + strand, within DVU2272 at 2363.193 kb on + strand, within DVU2272 at 2363.195 kb on + strand, within DVU2272 at 2363.214 kb on + strand, within DVU2272 at 2363.294 kb on + strand at 2363.294 kb on + strand at 2363.332 kb on + strand at 2363.436 kb on + strand at 2363.447 kb on + strand at 2363.461 kb on + strand at 2363.464 kb on - strand at 2363.479 kb on - strand at 2363.607 kb on + strand at 2363.657 kb on - strand at 2363.754 kb on + strand at 2363.921 kb on - strand at 2364.155 kb on + strand, within rocR at 2364.240 kb on + strand, within rocR at 2364.332 kb on - strand, within rocR at 2364.344 kb on + strand, within rocR at 2364.365 kb on + strand, within rocR at 2364.405 kb on - strand, within rocR at 2364.412 kb on - strand, within rocR at 2364.438 kb on + strand, within rocR
Per-strain Table
Position Strand Gene LocusTag Fraction MoLS4 with L-Glutamine (N) remove 2,360,076 - +0.6 2,360,086 - -0.1 2,360,088 + -0.4 2,360,096 - -0.7 2,360,116 - -0.2 2,360,116 - -0.2 2,360,143 - pflA DVU2271 0.12 -0.6 2,360,166 + pflA DVU2271 0.14 -0.9 2,360,181 + pflA DVU2271 0.16 +0.6 2,360,181 + pflA DVU2271 0.16 +0.2 2,360,181 + pflA DVU2271 0.16 +0.2 2,360,233 - pflA DVU2271 0.22 +0.3 2,360,293 - pflA DVU2271 0.28 +0.2 2,360,336 + pflA DVU2271 0.33 -4.5 2,360,407 + pflA DVU2271 0.41 -0.1 2,360,416 - pflA DVU2271 0.42 +0.8 2,360,487 + pflA DVU2271 0.50 +0.0 2,360,487 + pflA DVU2271 0.50 +0.0 2,360,495 - pflA DVU2271 0.51 -3.3 2,360,495 - pflA DVU2271 0.51 -0.1 2,360,595 + pflA DVU2271 0.62 -1.1 2,360,603 - pflA DVU2271 0.63 -2.4 2,360,613 + pflA DVU2271 0.64 -2.8 2,360,613 + pflA DVU2271 0.64 +0.0 2,360,621 - pflA DVU2271 0.65 -0.2 2,360,666 - pflA DVU2271 0.70 +0.1 2,360,666 - pflA DVU2271 0.70 -0.3 2,360,666 - pflA DVU2271 0.70 +0.0 2,360,766 + pflA DVU2271 0.81 -0.5 2,360,766 + pflA DVU2271 0.81 -3.5 2,360,766 + pflA DVU2271 0.81 -0.0 2,360,766 + pflA DVU2271 0.81 -0.2 2,360,766 + pflA DVU2271 0.81 -0.9 2,360,774 - pflA DVU2271 0.82 -3.7 2,360,774 - pflA DVU2271 0.82 -0.4 2,360,827 + pflA DVU2271 0.88 -3.4 2,360,837 + pflA DVU2271 0.89 -0.5 2,360,970 - -2.6 2,360,970 - -0.6 2,360,970 - -0.4 2,360,980 - -3.0 2,361,023 - +0.4 2,361,095 - +0.0 2,361,095 - +0.1 2,361,119 - -1.5 2,361,121 - -3.0 2,361,156 + +0.1 2,361,156 + -0.1 2,361,197 + +0.2 2,361,234 + -0.2 2,361,242 - -0.0 2,361,242 - -0.5 2,361,288 + -0.3 2,361,323 - DVU2272 0.11 +0.4 2,361,392 - DVU2272 0.14 -3.0 2,361,514 + DVU2272 0.19 -3.0 2,361,540 + DVU2272 0.20 -0.1 2,361,550 - DVU2272 0.21 -0.4 2,361,581 + DVU2272 0.22 -0.1 2,361,589 - DVU2272 0.22 -0.3 2,361,778 - DVU2272 0.30 +0.4 2,361,791 + DVU2272 0.30 +1.3 2,361,875 - DVU2272 0.34 -0.1 2,361,914 + DVU2272 0.35 -3.1 2,361,968 + DVU2272 0.38 -0.2 2,362,150 + DVU2272 0.45 -0.8 2,362,158 - DVU2272 0.45 +0.5 2,362,175 - DVU2272 0.46 -0.3 2,362,187 + DVU2272 0.47 +0.1 2,362,296 + DVU2272 0.51 -3.3 2,362,301 + DVU2272 0.51 +0.5 2,362,334 - DVU2272 0.53 +0.4 2,362,342 + DVU2272 0.53 +0.4 2,362,423 - DVU2272 0.56 -1.1 2,362,425 + DVU2272 0.56 -1.1 2,362,446 - DVU2272 0.57 +0.3 2,362,447 - DVU2272 0.57 -3.9 2,362,447 - DVU2272 0.57 +0.7 2,362,526 + DVU2272 0.60 -3.2 2,362,563 + DVU2272 0.62 +0.2 2,362,584 + DVU2272 0.63 +1.0 2,362,607 + DVU2272 0.64 +0.8 2,362,663 - DVU2272 0.66 -0.4 2,362,720 + DVU2272 0.68 +0.6 2,362,728 - DVU2272 0.69 -1.6 2,362,880 - DVU2272 0.75 -3.7 2,362,907 + DVU2272 0.76 -0.2 2,362,914 + DVU2272 0.76 +0.5 2,362,920 - DVU2272 0.76 +0.2 2,362,979 + DVU2272 0.79 -1.8 2,362,987 - DVU2272 0.79 -0.2 2,363,024 - DVU2272 0.81 -3.2 2,363,050 - DVU2272 0.82 -0.6 2,363,125 + DVU2272 0.85 +0.2 2,363,193 + DVU2272 0.88 +0.1 2,363,195 + DVU2272 0.88 -0.9 2,363,214 + DVU2272 0.88 -0.3 2,363,294 + -0.2 2,363,294 + -0.2 2,363,332 + +0.2 2,363,436 + -0.1 2,363,447 + -1.1 2,363,461 + +0.2 2,363,464 - -0.1 2,363,479 - -0.6 2,363,607 + +0.2 2,363,657 - -0.3 2,363,754 + -4.1 2,363,921 - +0.7 2,364,155 + rocR DVU2275 0.11 -0.2 2,364,240 + rocR DVU2275 0.17 +0.9 2,364,332 - rocR DVU2275 0.23 +0.1 2,364,344 + rocR DVU2275 0.24 -0.2 2,364,365 + rocR DVU2275 0.26 -1.1 2,364,405 - rocR DVU2275 0.28 -3.2 2,364,412 - rocR DVU2275 0.29 +2.0 2,364,438 + rocR DVU2275 0.31 -0.2
Or see this region's nucleotide sequence