Experiment: MoLS4 with L-Glutamine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt DVU2261 and tRNA-Ala are separated by 96 nucleotides tRNA-Ala and DVU2263 are separated by 455 nucleotides DVU2263 and DVU2264 are separated by 51 nucleotides DVU2264 and DVU2267 are separated by 304 nucleotides DVU2267 and DVU2268 are separated by 41 nucleotides
DVU2261: DVU2261 - hypothetical protein (TIGR), at 2,354,254 to 2,356,068
DVU2261
DVU_tRNA-Ala-5: tRNA-Ala - tRNA with anticodon CGC for Ala (VIMSS-AUTO), at 2,356,165 to 2,356,240
tRNA-Ala
DVU2263: DVU2263 - outer membrane autotransporter barrel domain protein (TIGR), at 2,356,696 to 2,358,621
DVU2263
DVU2264: DVU2264 - hypothetical protein (TIGR), at 2,358,673 to 2,358,825
DVU2264
DVU2267: DVU2267 - hypothetical protein (TIGR), at 2,359,130 to 2,359,423
DVU2267
DVU2268: DVU2268 - hypothetical protein (TIGR), at 2,359,465 to 2,359,584
DVU2268
Position (kb)
2356
2357
2358
2359 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1 at 2355.784 kb on - strand, within DVU2261 at 2355.887 kb on + strand at 2355.911 kb on + strand at 2355.912 kb on - strand at 2355.933 kb on - strand at 2355.950 kb on + strand at 2355.950 kb on + strand at 2356.008 kb on + strand at 2356.010 kb on + strand at 2356.016 kb on + strand at 2356.016 kb on - strand at 2356.024 kb on + strand at 2356.042 kb on + strand at 2356.056 kb on + strand at 2356.212 kb on - strand, within tRNA-Ala at 2356.238 kb on - strand at 2356.251 kb on + strand at 2356.305 kb on - strand at 2356.355 kb on + strand at 2356.375 kb on - strand at 2356.436 kb on - strand at 2356.470 kb on + strand at 2356.557 kb on + strand at 2356.727 kb on + strand at 2356.748 kb on + strand at 2356.748 kb on + strand at 2356.797 kb on + strand at 2356.808 kb on + strand at 2356.817 kb on - strand at 2356.889 kb on - strand, within DVU2263 at 2356.968 kb on + strand, within DVU2263 at 2356.968 kb on + strand, within DVU2263 at 2357.000 kb on - strand, within DVU2263 at 2357.006 kb on + strand, within DVU2263 at 2357.014 kb on - strand, within DVU2263 at 2357.043 kb on - strand, within DVU2263 at 2357.072 kb on + strand, within DVU2263 at 2357.104 kb on + strand, within DVU2263 at 2357.104 kb on + strand, within DVU2263 at 2357.112 kb on - strand, within DVU2263 at 2357.112 kb on - strand, within DVU2263 at 2357.112 kb on - strand, within DVU2263 at 2357.112 kb on - strand, within DVU2263 at 2357.125 kb on + strand, within DVU2263 at 2357.125 kb on + strand, within DVU2263 at 2357.133 kb on - strand, within DVU2263 at 2357.161 kb on - strand, within DVU2263 at 2357.236 kb on - strand, within DVU2263 at 2357.258 kb on + strand, within DVU2263 at 2357.281 kb on + strand, within DVU2263 at 2357.399 kb on - strand, within DVU2263 at 2357.399 kb on - strand, within DVU2263 at 2357.399 kb on - strand, within DVU2263 at 2357.399 kb on - strand, within DVU2263 at 2357.444 kb on - strand, within DVU2263 at 2357.466 kb on - strand, within DVU2263 at 2357.566 kb on + strand, within DVU2263 at 2357.698 kb on + strand, within DVU2263 at 2357.706 kb on - strand, within DVU2263 at 2357.764 kb on + strand, within DVU2263 at 2357.808 kb on + strand, within DVU2263 at 2357.808 kb on + strand, within DVU2263 at 2357.816 kb on - strand, within DVU2263 at 2357.816 kb on - strand, within DVU2263 at 2357.816 kb on - strand, within DVU2263 at 2357.853 kb on - strand, within DVU2263 at 2357.869 kb on + strand, within DVU2263 at 2357.877 kb on - strand, within DVU2263 at 2357.877 kb on - strand, within DVU2263 at 2358.101 kb on + strand, within DVU2263 at 2358.101 kb on + strand, within DVU2263 at 2358.101 kb on + strand, within DVU2263 at 2358.101 kb on + strand, within DVU2263 at 2358.111 kb on + strand, within DVU2263 at 2358.135 kb on + strand, within DVU2263 at 2358.135 kb on + strand, within DVU2263 at 2358.135 kb on + strand, within DVU2263 at 2358.137 kb on + strand, within DVU2263 at 2358.143 kb on - strand, within DVU2263 at 2358.143 kb on - strand, within DVU2263 at 2358.145 kb on - strand, within DVU2263 at 2358.218 kb on + strand, within DVU2263 at 2358.263 kb on + strand, within DVU2263 at 2358.265 kb on + strand, within DVU2263 at 2358.273 kb on + strand, within DVU2263 at 2358.273 kb on - strand, within DVU2263 at 2358.513 kb on - strand at 2358.566 kb on + strand at 2358.686 kb on + strand at 2358.687 kb on + strand at 2358.753 kb on - strand, within DVU2264 at 2358.898 kb on - strand at 2358.955 kb on + strand at 2358.963 kb on - strand at 2358.966 kb on + strand at 2358.974 kb on - strand at 2358.975 kb on - strand at 2358.976 kb on + strand at 2359.094 kb on - strand at 2359.100 kb on + strand at 2359.128 kb on + strand at 2359.156 kb on - strand at 2359.156 kb on - strand at 2359.156 kb on - strand at 2359.178 kb on - strand, within DVU2267 at 2359.206 kb on + strand, within DVU2267 at 2359.237 kb on + strand, within DVU2267 at 2359.308 kb on - strand, within DVU2267 at 2359.347 kb on - strand, within DVU2267 at 2359.416 kb on - strand at 2359.416 kb on - strand at 2359.475 kb on - strand at 2359.488 kb on + strand, within DVU2268 at 2359.593 kb on - strand at 2359.596 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction MoLS4 with L-Glutamine (N) remove 2,355,784 - DVU2261 0.84 +0.3 2,355,887 + -3.3 2,355,911 + -0.1 2,355,912 - -0.5 2,355,933 - +0.5 2,355,950 + -0.3 2,355,950 + -0.6 2,356,008 + -0.2 2,356,010 + -0.1 2,356,016 + -0.4 2,356,016 - -0.5 2,356,024 + -0.4 2,356,042 + +0.5 2,356,056 + +1.0 2,356,212 - tRNA-Ala DVU_tRNA-Ala-5 0.62 +0.2 2,356,238 - +0.2 2,356,251 + -1.1 2,356,305 - -3.4 2,356,355 + +0.1 2,356,375 - -3.2 2,356,436 - +0.6 2,356,470 + -0.1 2,356,557 + +0.6 2,356,727 + -0.3 2,356,748 + +0.0 2,356,748 + -0.5 2,356,797 + +0.4 2,356,808 + +0.1 2,356,817 - +0.7 2,356,889 - DVU2263 0.10 -2.0 2,356,968 + DVU2263 0.14 -0.6 2,356,968 + DVU2263 0.14 +0.1 2,357,000 - DVU2263 0.16 +1.1 2,357,006 + DVU2263 0.16 -0.1 2,357,014 - DVU2263 0.17 -0.8 2,357,043 - DVU2263 0.18 -0.9 2,357,072 + DVU2263 0.20 -0.1 2,357,104 + DVU2263 0.21 -0.3 2,357,104 + DVU2263 0.21 -0.4 2,357,112 - DVU2263 0.22 -2.9 2,357,112 - DVU2263 0.22 -0.2 2,357,112 - DVU2263 0.22 -4.4 2,357,112 - DVU2263 0.22 -0.3 2,357,125 + DVU2263 0.22 +0.7 2,357,125 + DVU2263 0.22 -0.4 2,357,133 - DVU2263 0.23 -3.3 2,357,161 - DVU2263 0.24 -0.3 2,357,236 - DVU2263 0.28 -0.4 2,357,258 + DVU2263 0.29 +0.0 2,357,281 + DVU2263 0.30 +1.3 2,357,399 - DVU2263 0.37 -0.2 2,357,399 - DVU2263 0.37 +0.2 2,357,399 - DVU2263 0.37 +0.5 2,357,399 - DVU2263 0.37 +0.4 2,357,444 - DVU2263 0.39 -0.1 2,357,466 - DVU2263 0.40 -0.1 2,357,566 + DVU2263 0.45 -0.2 2,357,698 + DVU2263 0.52 +0.1 2,357,706 - DVU2263 0.52 -0.1 2,357,764 + DVU2263 0.55 -0.2 2,357,808 + DVU2263 0.58 +0.7 2,357,808 + DVU2263 0.58 -0.2 2,357,816 - DVU2263 0.58 +0.1 2,357,816 - DVU2263 0.58 -0.3 2,357,816 - DVU2263 0.58 -0.4 2,357,853 - DVU2263 0.60 +0.7 2,357,869 + DVU2263 0.61 +0.4 2,357,877 - DVU2263 0.61 -0.0 2,357,877 - DVU2263 0.61 +1.5 2,358,101 + DVU2263 0.73 +0.3 2,358,101 + DVU2263 0.73 -1.0 2,358,101 + DVU2263 0.73 +0.5 2,358,101 + DVU2263 0.73 -0.1 2,358,111 + DVU2263 0.73 +0.2 2,358,135 + DVU2263 0.75 +0.3 2,358,135 + DVU2263 0.75 -1.2 2,358,135 + DVU2263 0.75 -0.3 2,358,137 + DVU2263 0.75 +0.5 2,358,143 - DVU2263 0.75 +1.3 2,358,143 - DVU2263 0.75 +0.3 2,358,145 - DVU2263 0.75 +0.3 2,358,218 + DVU2263 0.79 +0.7 2,358,263 + DVU2263 0.81 -1.3 2,358,265 + DVU2263 0.81 +1.2 2,358,273 + DVU2263 0.82 -0.1 2,358,273 - DVU2263 0.82 +0.9 2,358,513 - +0.1 2,358,566 + +0.4 2,358,686 + -0.4 2,358,687 + +0.2 2,358,753 - DVU2264 0.52 -0.6 2,358,898 - -4.8 2,358,955 + -0.1 2,358,963 - -1.1 2,358,966 + +1.7 2,358,974 - -3.5 2,358,975 - -0.4 2,358,976 + +0.2 2,359,094 - -0.4 2,359,100 + +1.1 2,359,128 + -3.4 2,359,156 - -0.6 2,359,156 - +1.4 2,359,156 - +0.8 2,359,178 - DVU2267 0.16 -0.2 2,359,206 + DVU2267 0.26 -2.6 2,359,237 + DVU2267 0.36 -0.1 2,359,308 - DVU2267 0.61 +0.9 2,359,347 - DVU2267 0.74 +0.4 2,359,416 - +0.8 2,359,416 - +0.2 2,359,475 - +0.1 2,359,488 + DVU2268 0.19 -0.2 2,359,593 - +0.9 2,359,596 - -1.1
Or see this region's nucleotide sequence