Experiment: MoLS4 with L-Glutamine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt proS and xseA are separated by 46 nucleotides xseA and DVU1347 are separated by 28 nucleotides DVU1347 and xseB are separated by 11 nucleotides
DVU1345: proS - prolyl-tRNA synthetase (TIGR), at 1,422,321 to 1,424,045
proS
DVU1346: xseA - exodeoxyribonuclease VII, large subunit (TIGR), at 1,424,092 to 1,425,489
xseA
DVU1347: DVU1347 - peptidase, M23/M37 family (TIGR), at 1,425,518 to 1,426,432
DVU1347
DVU1348: xseB - exodeoxyribonuclease VII, small subunit (TIGR), at 1,426,444 to 1,426,689
xseB
Position (kb)
1424
1425
1426 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 1424.108 kb on - strand at 1424.245 kb on + strand, within xseA at 1424.245 kb on + strand, within xseA at 1424.291 kb on + strand, within xseA at 1424.322 kb on - strand, within xseA at 1424.376 kb on - strand, within xseA at 1424.386 kb on - strand, within xseA at 1424.391 kb on - strand, within xseA at 1424.403 kb on - strand, within xseA at 1424.482 kb on + strand, within xseA at 1424.643 kb on + strand, within xseA at 1424.658 kb on + strand, within xseA at 1424.723 kb on + strand, within xseA at 1424.973 kb on + strand, within xseA at 1425.026 kb on - strand, within xseA at 1425.033 kb on - strand, within xseA at 1425.064 kb on + strand, within xseA at 1425.185 kb on + strand, within xseA at 1425.241 kb on + strand, within xseA at 1425.241 kb on + strand, within xseA at 1425.254 kb on - strand, within xseA at 1425.275 kb on + strand, within xseA at 1425.283 kb on - strand, within xseA at 1425.412 kb on - strand at 1425.501 kb on - strand at 1425.528 kb on + strand at 1425.559 kb on + strand at 1425.574 kb on + strand at 1425.577 kb on - strand at 1425.704 kb on - strand, within DVU1347 at 1425.738 kb on - strand, within DVU1347 at 1425.862 kb on + strand, within DVU1347 at 1425.893 kb on - strand, within DVU1347 at 1425.897 kb on + strand, within DVU1347 at 1426.020 kb on - strand, within DVU1347 at 1426.158 kb on + strand, within DVU1347 at 1426.347 kb on + strand at 1426.440 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction MoLS4 with L-Glutamine (N) remove 1,424,108 - +0.4 1,424,245 + xseA DVU1346 0.11 -2.3 1,424,245 + xseA DVU1346 0.11 +0.1 1,424,291 + xseA DVU1346 0.14 -0.6 1,424,322 - xseA DVU1346 0.16 -3.5 1,424,376 - xseA DVU1346 0.20 -0.2 1,424,386 - xseA DVU1346 0.21 +1.0 1,424,391 - xseA DVU1346 0.21 -0.7 1,424,403 - xseA DVU1346 0.22 -1.2 1,424,482 + xseA DVU1346 0.28 -0.4 1,424,643 + xseA DVU1346 0.39 -2.2 1,424,658 + xseA DVU1346 0.40 -0.1 1,424,723 + xseA DVU1346 0.45 +0.1 1,424,973 + xseA DVU1346 0.63 -1.0 1,425,026 - xseA DVU1346 0.67 -2.7 1,425,033 - xseA DVU1346 0.67 +0.0 1,425,064 + xseA DVU1346 0.70 -0.5 1,425,185 + xseA DVU1346 0.78 -4.6 1,425,241 + xseA DVU1346 0.82 +0.4 1,425,241 + xseA DVU1346 0.82 -2.8 1,425,254 - xseA DVU1346 0.83 -0.3 1,425,275 + xseA DVU1346 0.85 +0.9 1,425,283 - xseA DVU1346 0.85 -3.0 1,425,412 - -3.2 1,425,501 - +1.2 1,425,528 + -0.2 1,425,559 + -0.6 1,425,574 + -0.2 1,425,577 - -0.2 1,425,704 - DVU1347 0.20 +0.3 1,425,738 - DVU1347 0.24 -1.2 1,425,862 + DVU1347 0.38 +0.5 1,425,893 - DVU1347 0.41 -2.9 1,425,897 + DVU1347 0.41 -0.7 1,426,020 - DVU1347 0.55 +0.5 1,426,158 + DVU1347 0.70 +0.2 1,426,347 + -0.9 1,426,440 + +1.1
Or see this region's nucleotide sequence