Strain Fitness in Rhodanobacter denitrificans FW104-10B01 around LRK54_RS15115

Experiment: R2A with Chromium(III) Chloride Hexahydrate 0.09765625 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntLRK54_RS15105 and LRK54_RS15110 overlap by 4 nucleotidesLRK54_RS15110 and LRK54_RS15115 are separated by 55 nucleotidesLRK54_RS15115 and LRK54_RS15120 are separated by 17 nucleotidesLRK54_RS15120 and LRK54_RS15125 are separated by 100 nucleotides LRK54_RS15105: LRK54_RS15105 - polynucleotide adenylyltransferase PcnB, at 3,336,120 to 3,337,472 _RS15105 LRK54_RS15110: LRK54_RS15110 - 2-amino-4-hydroxy-6- hydroxymethyldihydropteridine diphosphokinase, at 3,337,469 to 3,337,948 _RS15110 LRK54_RS15115: LRK54_RS15115 - 3-methyl-2-oxobutanoate hydroxymethyltransferase, at 3,338,004 to 3,338,834 _RS15115 LRK54_RS15120: LRK54_RS15120 - pantoate--beta-alanine ligase, at 3,338,852 to 3,339,697 _RS15120 LRK54_RS15125: LRK54_RS15125 - aspartate 1-decarboxylase, at 3,339,798 to 3,340,190 _RS15125 Position (kb) 3338 3339Strain fitness (log2 ratio) -2 -1 0 1at 3337.481 kb on + strandat 3337.971 kb on + strandat 3338.007 kb on + strandat 3339.708 kb on + strandat 3339.756 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction R2A with Chromium(III) Chloride Hexahydrate 0.09765625 mM
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3,337,481 + -0.6
3,337,971 + +1.7
3,338,007 + -2.0
3,339,708 + +0.6
3,339,756 + -1.3

Or see this region's nucleotide sequence