Experiment: R2A with Chromium(III) Chloride Hexahydrate 0.09765625 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt LRK54_RS06015 and LRK54_RS06020 overlap by 4 nucleotides LRK54_RS06020 and LRK54_RS06025 are separated by 10 nucleotides LRK54_RS06025 and LRK54_RS06030 are separated by 8 nucleotides LRK54_RS06030 and LRK54_RS06035 overlap by 4 nucleotides
LRK54_RS06015: LRK54_RS06015 - putative baseplate assembly protein, at 1,296,646 to 1,299,369
_RS06015
LRK54_RS06020: LRK54_RS06020 - GPW/gp25 family protein, at 1,299,366 to 1,299,725
_RS06020
LRK54_RS06025: LRK54_RS06025 - hypothetical protein, at 1,299,736 to 1,300,062
_RS06025
LRK54_RS06030: LRK54_RS06030 - phage baseplate assembly protein V, at 1,300,071 to 1,300,577
_RS06030
LRK54_RS06035: LRK54_RS06035 - hypothetical protein, at 1,300,574 to 1,301,710
_RS06035
Position (kb)
1300
1301 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 1299.249 kb on - strand at 1299.249 kb on - strand at 1299.249 kb on - strand at 1299.249 kb on - strand at 1299.249 kb on - strand at 1299.249 kb on - strand at 1299.336 kb on + strand at 1299.423 kb on - strand, within LRK54_RS06020 at 1299.424 kb on + strand, within LRK54_RS06020 at 1299.424 kb on + strand, within LRK54_RS06020 at 1299.424 kb on + strand, within LRK54_RS06020 at 1299.424 kb on + strand, within LRK54_RS06020 at 1299.424 kb on + strand, within LRK54_RS06020 at 1299.424 kb on + strand, within LRK54_RS06020 at 1299.425 kb on - strand, within LRK54_RS06020 at 1299.425 kb on - strand, within LRK54_RS06020 at 1299.425 kb on - strand, within LRK54_RS06020 at 1299.425 kb on - strand, within LRK54_RS06020 at 1299.425 kb on - strand, within LRK54_RS06020 at 1299.425 kb on - strand, within LRK54_RS06020 at 1299.425 kb on - strand, within LRK54_RS06020 at 1299.425 kb on - strand, within LRK54_RS06020 at 1300.072 kb on + strand at 1300.072 kb on + strand at 1300.072 kb on + strand at 1300.072 kb on + strand at 1300.072 kb on + strand at 1300.072 kb on + strand at 1300.073 kb on - strand at 1300.073 kb on - strand at 1300.073 kb on - strand at 1300.073 kb on - strand at 1300.358 kb on - strand, within LRK54_RS06030 at 1300.417 kb on + strand, within LRK54_RS06030 at 1300.417 kb on + strand, within LRK54_RS06030 at 1300.418 kb on - strand, within LRK54_RS06030 at 1300.418 kb on - strand, within LRK54_RS06030 at 1300.418 kb on - strand, within LRK54_RS06030 at 1300.547 kb on + strand at 1300.555 kb on + strand at 1300.555 kb on + strand at 1300.555 kb on + strand at 1300.555 kb on + strand at 1300.556 kb on - strand at 1300.556 kb on - strand at 1300.677 kb on + strand at 1300.677 kb on + strand at 1300.677 kb on + strand at 1300.677 kb on + strand at 1300.677 kb on + strand at 1300.677 kb on + strand at 1300.677 kb on + strand at 1300.677 kb on + strand at 1300.677 kb on + strand at 1300.677 kb on + strand at 1300.678 kb on - strand at 1300.678 kb on - strand at 1300.678 kb on - strand at 1300.678 kb on - strand at 1300.678 kb on - strand at 1300.678 kb on - strand at 1300.678 kb on - strand at 1300.678 kb on - strand at 1300.678 kb on - strand at 1300.678 kb on - strand at 1300.842 kb on + strand, within LRK54_RS06035 at 1300.842 kb on + strand, within LRK54_RS06035 at 1300.842 kb on + strand, within LRK54_RS06035 at 1300.842 kb on + strand, within LRK54_RS06035 at 1300.843 kb on - strand, within LRK54_RS06035 at 1300.843 kb on - strand, within LRK54_RS06035 at 1300.843 kb on - strand, within LRK54_RS06035 at 1300.843 kb on - strand, within LRK54_RS06035 at 1300.843 kb on - strand, within LRK54_RS06035 at 1300.971 kb on + strand, within LRK54_RS06035 at 1300.971 kb on + strand, within LRK54_RS06035 at 1300.971 kb on + strand, within LRK54_RS06035 at 1300.971 kb on + strand, within LRK54_RS06035 at 1300.971 kb on + strand, within LRK54_RS06035 at 1300.972 kb on - strand, within LRK54_RS06035 at 1300.972 kb on - strand, within LRK54_RS06035 at 1300.972 kb on - strand, within LRK54_RS06035 at 1300.972 kb on - strand, within LRK54_RS06035 at 1300.972 kb on - strand, within LRK54_RS06035 at 1301.004 kb on + strand, within LRK54_RS06035 at 1301.075 kb on + strand, within LRK54_RS06035 at 1301.075 kb on + strand, within LRK54_RS06035 at 1301.075 kb on + strand, within LRK54_RS06035 at 1301.075 kb on + strand, within LRK54_RS06035 at 1301.076 kb on - strand, within LRK54_RS06035 at 1301.076 kb on - strand, within LRK54_RS06035 at 1301.076 kb on - strand, within LRK54_RS06035 at 1301.076 kb on - strand, within LRK54_RS06035 at 1301.076 kb on - strand, within LRK54_RS06035 at 1301.076 kb on - strand, within LRK54_RS06035 at 1301.076 kb on - strand, within LRK54_RS06035 at 1301.136 kb on + strand, within LRK54_RS06035 at 1301.136 kb on + strand, within LRK54_RS06035 at 1301.136 kb on + strand, within LRK54_RS06035 at 1301.137 kb on - strand, within LRK54_RS06035 at 1301.137 kb on - strand, within LRK54_RS06035 at 1301.137 kb on - strand, within LRK54_RS06035 at 1301.137 kb on - strand, within LRK54_RS06035 at 1301.145 kb on + strand, within LRK54_RS06035 at 1301.146 kb on - strand, within LRK54_RS06035 at 1301.146 kb on - strand, within LRK54_RS06035 at 1301.146 kb on - strand, within LRK54_RS06035 at 1301.175 kb on + strand, within LRK54_RS06035 at 1301.175 kb on + strand, within LRK54_RS06035 at 1301.175 kb on + strand, within LRK54_RS06035 at 1301.175 kb on + strand, within LRK54_RS06035 at 1301.175 kb on + strand, within LRK54_RS06035 at 1301.175 kb on + strand, within LRK54_RS06035 at 1301.176 kb on - strand, within LRK54_RS06035 at 1301.176 kb on - strand, within LRK54_RS06035 at 1301.176 kb on - strand, within LRK54_RS06035 at 1301.176 kb on - strand, within LRK54_RS06035 at 1301.176 kb on - strand, within LRK54_RS06035 at 1301.176 kb on - strand, within LRK54_RS06035 at 1301.176 kb on - strand, within LRK54_RS06035 at 1301.176 kb on - strand, within LRK54_RS06035 at 1301.176 kb on - strand, within LRK54_RS06035 at 1301.176 kb on - strand, within LRK54_RS06035 at 1301.176 kb on - strand, within LRK54_RS06035 at 1301.206 kb on + strand, within LRK54_RS06035 at 1301.206 kb on + strand, within LRK54_RS06035 at 1301.206 kb on + strand, within LRK54_RS06035 at 1301.206 kb on + strand, within LRK54_RS06035 at 1301.207 kb on - strand, within LRK54_RS06035 at 1301.207 kb on - strand, within LRK54_RS06035 at 1301.277 kb on + strand, within LRK54_RS06035 at 1301.277 kb on + strand, within LRK54_RS06035 at 1301.277 kb on + strand, within LRK54_RS06035 at 1301.277 kb on + strand, within LRK54_RS06035 at 1301.277 kb on + strand, within LRK54_RS06035 at 1301.277 kb on + strand, within LRK54_RS06035 at 1301.277 kb on + strand, within LRK54_RS06035 at 1301.277 kb on + strand, within LRK54_RS06035 at 1301.278 kb on - strand, within LRK54_RS06035 at 1301.278 kb on - strand, within LRK54_RS06035 at 1301.278 kb on - strand, within LRK54_RS06035 at 1301.278 kb on - strand, within LRK54_RS06035 at 1301.404 kb on + strand, within LRK54_RS06035 at 1301.404 kb on + strand, within LRK54_RS06035 at 1301.404 kb on + strand, within LRK54_RS06035 at 1301.404 kb on + strand, within LRK54_RS06035 at 1301.404 kb on + strand, within LRK54_RS06035 at 1301.405 kb on - strand, within LRK54_RS06035
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Chromium(III) Chloride Hexahydrate 0.09765625 mM remove 1,299,249 - +2.1 1,299,249 - -0.4 1,299,249 - +0.3 1,299,249 - -0.4 1,299,249 - +0.3 1,299,249 - +2.1 1,299,336 + -1.1 1,299,423 - LRK54_RS06020 0.16 -0.6 1,299,424 + LRK54_RS06020 0.16 -0.6 1,299,424 + LRK54_RS06020 0.16 -0.6 1,299,424 + LRK54_RS06020 0.16 +0.6 1,299,424 + LRK54_RS06020 0.16 +1.1 1,299,424 + LRK54_RS06020 0.16 +0.8 1,299,424 + LRK54_RS06020 0.16 -0.7 1,299,425 - LRK54_RS06020 0.16 -2.0 1,299,425 - LRK54_RS06020 0.16 +0.8 1,299,425 - LRK54_RS06020 0.16 +2.1 1,299,425 - LRK54_RS06020 0.16 -0.6 1,299,425 - LRK54_RS06020 0.16 -0.2 1,299,425 - LRK54_RS06020 0.16 +0.6 1,299,425 - LRK54_RS06020 0.16 -0.0 1,299,425 - LRK54_RS06020 0.16 -1.0 1,300,072 + -0.7 1,300,072 + -0.1 1,300,072 + -1.3 1,300,072 + -0.4 1,300,072 + +1.6 1,300,072 + -0.3 1,300,073 - -1.5 1,300,073 - -0.2 1,300,073 - +2.1 1,300,073 - -1.6 1,300,358 - LRK54_RS06030 0.57 -1.4 1,300,417 + LRK54_RS06030 0.68 -0.5 1,300,417 + LRK54_RS06030 0.68 +0.3 1,300,418 - LRK54_RS06030 0.68 -1.5 1,300,418 - LRK54_RS06030 0.68 -0.6 1,300,418 - LRK54_RS06030 0.68 -0.9 1,300,547 + +1.1 1,300,555 + +0.9 1,300,555 + -2.0 1,300,555 + -2.6 1,300,555 + -0.7 1,300,556 - -0.7 1,300,556 - -1.0 1,300,677 + +1.1 1,300,677 + -1.7 1,300,677 + +1.2 1,300,677 + -0.3 1,300,677 + +0.3 1,300,677 + +0.4 1,300,677 + +2.1 1,300,677 + +0.3 1,300,677 + -1.6 1,300,677 + +3.1 1,300,678 - +0.2 1,300,678 - -1.7 1,300,678 - +1.1 1,300,678 - +1.4 1,300,678 - -0.2 1,300,678 - -2.3 1,300,678 - -2.5 1,300,678 - +0.5 1,300,678 - -0.0 1,300,678 - -0.1 1,300,842 + LRK54_RS06035 0.24 -0.8 1,300,842 + LRK54_RS06035 0.24 -0.7 1,300,842 + LRK54_RS06035 0.24 +0.6 1,300,842 + LRK54_RS06035 0.24 +0.1 1,300,843 - LRK54_RS06035 0.24 -0.0 1,300,843 - LRK54_RS06035 0.24 +1.6 1,300,843 - LRK54_RS06035 0.24 +0.6 1,300,843 - LRK54_RS06035 0.24 +1.3 1,300,843 - LRK54_RS06035 0.24 -0.4 1,300,971 + LRK54_RS06035 0.35 -1.3 1,300,971 + LRK54_RS06035 0.35 -1.9 1,300,971 + LRK54_RS06035 0.35 -1.4 1,300,971 + LRK54_RS06035 0.35 +0.9 1,300,971 + LRK54_RS06035 0.35 +0.3 1,300,972 - LRK54_RS06035 0.35 -0.2 1,300,972 - LRK54_RS06035 0.35 -0.8 1,300,972 - LRK54_RS06035 0.35 +0.3 1,300,972 - LRK54_RS06035 0.35 +0.1 1,300,972 - LRK54_RS06035 0.35 -0.5 1,301,004 + LRK54_RS06035 0.38 -2.3 1,301,075 + LRK54_RS06035 0.44 +2.1 1,301,075 + LRK54_RS06035 0.44 -0.3 1,301,075 + LRK54_RS06035 0.44 -0.2 1,301,075 + LRK54_RS06035 0.44 -0.9 1,301,076 - LRK54_RS06035 0.44 +0.1 1,301,076 - LRK54_RS06035 0.44 -1.2 1,301,076 - LRK54_RS06035 0.44 -0.2 1,301,076 - LRK54_RS06035 0.44 -1.1 1,301,076 - LRK54_RS06035 0.44 -0.4 1,301,076 - LRK54_RS06035 0.44 -1.8 1,301,076 - LRK54_RS06035 0.44 -0.4 1,301,136 + LRK54_RS06035 0.49 +1.0 1,301,136 + LRK54_RS06035 0.49 -0.9 1,301,136 + LRK54_RS06035 0.49 -0.2 1,301,137 - LRK54_RS06035 0.50 -1.5 1,301,137 - LRK54_RS06035 0.50 +0.8 1,301,137 - LRK54_RS06035 0.50 +0.8 1,301,137 - LRK54_RS06035 0.50 -1.9 1,301,145 + LRK54_RS06035 0.50 -1.9 1,301,146 - LRK54_RS06035 0.50 -1.1 1,301,146 - LRK54_RS06035 0.50 -0.0 1,301,146 - LRK54_RS06035 0.50 -0.2 1,301,175 + LRK54_RS06035 0.53 -0.2 1,301,175 + LRK54_RS06035 0.53 -0.7 1,301,175 + LRK54_RS06035 0.53 -1.9 1,301,175 + LRK54_RS06035 0.53 -1.4 1,301,175 + LRK54_RS06035 0.53 +0.6 1,301,175 + LRK54_RS06035 0.53 -0.3 1,301,176 - LRK54_RS06035 0.53 +1.3 1,301,176 - LRK54_RS06035 0.53 -0.9 1,301,176 - LRK54_RS06035 0.53 -0.0 1,301,176 - LRK54_RS06035 0.53 -1.0 1,301,176 - LRK54_RS06035 0.53 +0.1 1,301,176 - LRK54_RS06035 0.53 +1.1 1,301,176 - LRK54_RS06035 0.53 -2.0 1,301,176 - LRK54_RS06035 0.53 +0.3 1,301,176 - LRK54_RS06035 0.53 -2.3 1,301,176 - LRK54_RS06035 0.53 -0.3 1,301,176 - LRK54_RS06035 0.53 -0.8 1,301,206 + LRK54_RS06035 0.56 +1.8 1,301,206 + LRK54_RS06035 0.56 -0.6 1,301,206 + LRK54_RS06035 0.56 +0.8 1,301,206 + LRK54_RS06035 0.56 -0.0 1,301,207 - LRK54_RS06035 0.56 +0.3 1,301,207 - LRK54_RS06035 0.56 +2.1 1,301,277 + LRK54_RS06035 0.62 -0.9 1,301,277 + LRK54_RS06035 0.62 -1.3 1,301,277 + LRK54_RS06035 0.62 +2.1 1,301,277 + LRK54_RS06035 0.62 +1.1 1,301,277 + LRK54_RS06035 0.62 -0.6 1,301,277 + LRK54_RS06035 0.62 -0.8 1,301,277 + LRK54_RS06035 0.62 +0.8 1,301,277 + LRK54_RS06035 0.62 +0.3 1,301,278 - LRK54_RS06035 0.62 -2.6 1,301,278 - LRK54_RS06035 0.62 -0.8 1,301,278 - LRK54_RS06035 0.62 -1.6 1,301,278 - LRK54_RS06035 0.62 -0.0 1,301,404 + LRK54_RS06035 0.73 -0.2 1,301,404 + LRK54_RS06035 0.73 -0.6 1,301,404 + LRK54_RS06035 0.73 -0.6 1,301,404 + LRK54_RS06035 0.73 -0.0 1,301,404 + LRK54_RS06035 0.73 +0.3 1,301,405 - LRK54_RS06035 0.73 +0.8
Or see this region's nucleotide sequence