Experiment: R2A with Chromium(III) Chloride Hexahydrate 0.09765625 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt LRK54_RS04360 and LRK54_RS04365 are separated by 277 nucleotides LRK54_RS04365 and LRK54_RS04370 are separated by 174 nucleotides LRK54_RS04370 and LRK54_RS04375 overlap by 4 nucleotides LRK54_RS04375 and LRK54_RS04380 are separated by 10 nucleotides
LRK54_RS04360: LRK54_RS04360 - proline--tRNA ligase, at 919,657 to 921,354
_RS04360
LRK54_RS04365: LRK54_RS04365 - H-NS histone family protein, at 921,632 to 921,955
_RS04365
LRK54_RS04370: LRK54_RS04370 - threonine/serine exporter family protein, at 922,130 to 923,410
_RS04370
LRK54_RS04375: LRK54_RS04375 - NfeD family protein, at 923,407 to 923,853
_RS04375
LRK54_RS04380: LRK54_RS04380 - SPFH/Band 7/PHB domain protein, at 923,864 to 924,808
_RS04380
Position (kb) 922
923
924 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2
3 at 921.371 kb on + strand at 921.372 kb on - strand at 921.372 kb on - strand at 921.372 kb on - strand at 921.439 kb on + strand at 921.439 kb on + strand at 921.439 kb on + strand at 921.440 kb on - strand at 921.463 kb on - strand at 921.463 kb on - strand at 921.481 kb on + strand at 921.482 kb on - strand at 921.482 kb on - strand at 921.482 kb on - strand at 921.509 kb on + strand at 921.515 kb on + strand at 921.516 kb on - strand at 921.526 kb on + strand at 921.577 kb on + strand at 921.577 kb on + strand at 921.577 kb on + strand at 921.577 kb on + strand at 921.580 kb on + strand at 921.581 kb on - strand at 921.581 kb on - strand at 921.604 kb on + strand at 921.604 kb on + strand at 921.617 kb on + strand at 921.618 kb on - strand at 921.643 kb on + strand at 921.644 kb on - strand at 921.774 kb on - strand, within LRK54_RS04365 at 921.845 kb on + strand, within LRK54_RS04365 at 921.846 kb on - strand, within LRK54_RS04365 at 922.131 kb on + strand at 922.131 kb on + strand at 922.131 kb on + strand at 922.131 kb on + strand at 922.131 kb on + strand at 922.132 kb on - strand at 922.132 kb on - strand at 922.132 kb on - strand at 922.132 kb on - strand at 922.132 kb on - strand at 922.132 kb on - strand at 922.157 kb on + strand at 922.157 kb on + strand at 922.157 kb on + strand at 922.158 kb on - strand at 922.158 kb on - strand at 922.158 kb on - strand at 922.158 kb on - strand at 922.347 kb on + strand, within LRK54_RS04370 at 922.348 kb on - strand, within LRK54_RS04370 at 922.348 kb on - strand, within LRK54_RS04370 at 922.449 kb on + strand, within LRK54_RS04370 at 922.449 kb on + strand, within LRK54_RS04370 at 922.449 kb on + strand, within LRK54_RS04370 at 922.449 kb on + strand, within LRK54_RS04370 at 922.449 kb on + strand, within LRK54_RS04370 at 922.450 kb on - strand, within LRK54_RS04370 at 922.989 kb on + strand, within LRK54_RS04370 at 922.989 kb on + strand, within LRK54_RS04370 at 922.989 kb on + strand, within LRK54_RS04370 at 922.989 kb on + strand, within LRK54_RS04370 at 922.989 kb on + strand, within LRK54_RS04370 at 922.989 kb on + strand, within LRK54_RS04370 at 922.990 kb on - strand, within LRK54_RS04370 at 922.990 kb on - strand, within LRK54_RS04370 at 923.310 kb on + strand at 923.310 kb on + strand at 923.311 kb on - strand at 923.452 kb on + strand, within LRK54_RS04375 at 923.452 kb on + strand, within LRK54_RS04375 at 923.452 kb on + strand, within LRK54_RS04375 at 923.452 kb on + strand, within LRK54_RS04375 at 923.452 kb on + strand, within LRK54_RS04375 at 923.452 kb on + strand, within LRK54_RS04375 at 923.452 kb on + strand, within LRK54_RS04375 at 923.453 kb on - strand, within LRK54_RS04375 at 923.453 kb on - strand, within LRK54_RS04375 at 923.453 kb on - strand, within LRK54_RS04375 at 923.453 kb on - strand, within LRK54_RS04375 at 923.453 kb on - strand, within LRK54_RS04375 at 923.453 kb on - strand, within LRK54_RS04375 at 923.453 kb on - strand, within LRK54_RS04375 at 923.453 kb on - strand, within LRK54_RS04375 at 923.453 kb on - strand, within LRK54_RS04375 at 923.558 kb on + strand, within LRK54_RS04375 at 923.558 kb on + strand, within LRK54_RS04375 at 923.558 kb on + strand, within LRK54_RS04375 at 923.559 kb on - strand, within LRK54_RS04375 at 923.559 kb on - strand, within LRK54_RS04375 at 923.559 kb on - strand, within LRK54_RS04375 at 923.559 kb on - strand, within LRK54_RS04375 at 923.674 kb on + strand, within LRK54_RS04375 at 923.674 kb on + strand, within LRK54_RS04375 at 923.674 kb on + strand, within LRK54_RS04375 at 923.675 kb on - strand, within LRK54_RS04375 at 923.675 kb on - strand, within LRK54_RS04375 at 923.774 kb on + strand, within LRK54_RS04375 at 923.774 kb on + strand, within LRK54_RS04375 at 923.774 kb on + strand, within LRK54_RS04375 at 923.774 kb on + strand, within LRK54_RS04375 at 923.775 kb on - strand, within LRK54_RS04375 at 923.775 kb on - strand, within LRK54_RS04375 at 923.831 kb on + strand at 923.831 kb on + strand at 923.831 kb on + strand at 923.831 kb on + strand at 923.831 kb on + strand at 923.831 kb on + strand at 923.831 kb on + strand at 923.831 kb on + strand at 923.831 kb on + strand at 923.831 kb on + strand at 923.831 kb on + strand at 923.832 kb on - strand at 923.832 kb on - strand at 923.832 kb on - strand at 923.832 kb on - strand at 923.832 kb on - strand at 923.832 kb on - strand at 923.832 kb on - strand at 923.832 kb on - strand at 923.832 kb on - strand at 923.832 kb on - strand at 923.832 kb on - strand at 923.832 kb on - strand at 924.030 kb on + strand, within LRK54_RS04380 at 924.030 kb on + strand, within LRK54_RS04380 at 924.030 kb on + strand, within LRK54_RS04380 at 924.030 kb on + strand, within LRK54_RS04380 at 924.030 kb on + strand, within LRK54_RS04380 at 924.030 kb on + strand, within LRK54_RS04380 at 924.030 kb on + strand, within LRK54_RS04380 at 924.030 kb on + strand, within LRK54_RS04380 at 924.030 kb on + strand, within LRK54_RS04380 at 924.030 kb on + strand, within LRK54_RS04380 at 924.030 kb on + strand, within LRK54_RS04380 at 924.031 kb on - strand, within LRK54_RS04380 at 924.031 kb on - strand, within LRK54_RS04380 at 924.031 kb on - strand, within LRK54_RS04380 at 924.031 kb on - strand, within LRK54_RS04380 at 924.031 kb on - strand, within LRK54_RS04380 at 924.031 kb on - strand, within LRK54_RS04380 at 924.031 kb on - strand, within LRK54_RS04380 at 924.031 kb on - strand, within LRK54_RS04380 at 924.031 kb on - strand, within LRK54_RS04380 at 924.031 kb on - strand, within LRK54_RS04380 at 924.031 kb on - strand, within LRK54_RS04380 at 924.031 kb on - strand, within LRK54_RS04380 at 924.048 kb on + strand, within LRK54_RS04380 at 924.048 kb on + strand, within LRK54_RS04380 at 924.048 kb on + strand, within LRK54_RS04380 at 924.048 kb on + strand, within LRK54_RS04380 at 924.048 kb on + strand, within LRK54_RS04380 at 924.049 kb on - strand, within LRK54_RS04380 at 924.049 kb on - strand, within LRK54_RS04380 at 924.049 kb on - strand, within LRK54_RS04380 at 924.049 kb on - strand, within LRK54_RS04380 at 924.049 kb on - strand, within LRK54_RS04380 at 924.049 kb on - strand, within LRK54_RS04380 at 924.049 kb on - strand, within LRK54_RS04380 at 924.049 kb on - strand, within LRK54_RS04380 at 924.049 kb on - strand, within LRK54_RS04380 at 924.049 kb on - strand, within LRK54_RS04380 at 924.049 kb on - strand, within LRK54_RS04380
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Chromium(III) Chloride Hexahydrate 0.09765625 mM remove 921,371 + +1.6 921,372 - +0.4 921,372 - +0.6 921,372 - -0.6 921,439 + -0.9 921,439 + -3.1 921,439 + -0.3 921,440 - -1.6 921,463 - -0.5 921,463 - -0.4 921,481 + +0.9 921,482 - +0.6 921,482 - -1.8 921,482 - -1.2 921,509 + -0.7 921,515 + -0.3 921,516 - -3.0 921,526 + +1.6 921,577 + -0.6 921,577 + -0.1 921,577 + -1.6 921,577 + -1.8 921,580 + +1.6 921,581 - -1.0 921,581 - -2.2 921,604 + -0.6 921,604 + -0.1 921,617 + -1.7 921,618 - +0.0 921,643 + -0.5 921,644 - -0.9 921,774 - LRK54_RS04365 0.44 +0.2 921,845 + LRK54_RS04365 0.66 +1.6 921,846 - LRK54_RS04365 0.66 -1.3 922,131 + -0.1 922,131 + -1.5 922,131 + +0.0 922,131 + -0.3 922,131 + +0.4 922,132 - +1.6 922,132 - -1.9 922,132 - +0.4 922,132 - -0.4 922,132 - +1.6 922,132 - -1.3 922,157 + -0.9 922,157 + +0.4 922,157 + -1.0 922,158 - +3.2 922,158 - +0.0 922,158 - -2.6 922,158 - -0.5 922,347 + LRK54_RS04370 0.17 +0.9 922,348 - LRK54_RS04370 0.17 -2.7 922,348 - LRK54_RS04370 0.17 +0.9 922,449 + LRK54_RS04370 0.25 +0.9 922,449 + LRK54_RS04370 0.25 +0.4 922,449 + LRK54_RS04370 0.25 -0.4 922,449 + LRK54_RS04370 0.25 -0.3 922,449 + LRK54_RS04370 0.25 +2.2 922,450 - LRK54_RS04370 0.25 -2.0 922,989 + LRK54_RS04370 0.67 -1.3 922,989 + LRK54_RS04370 0.67 -0.3 922,989 + LRK54_RS04370 0.67 -2.0 922,989 + LRK54_RS04370 0.67 -1.1 922,989 + LRK54_RS04370 0.67 -1.1 922,989 + LRK54_RS04370 0.67 -0.1 922,990 - LRK54_RS04370 0.67 +1.2 922,990 - LRK54_RS04370 0.67 -1.6 923,310 + -0.4 923,310 + +0.6 923,311 - +0.4 923,452 + LRK54_RS04375 0.10 +0.2 923,452 + LRK54_RS04375 0.10 -0.1 923,452 + LRK54_RS04375 0.10 +0.0 923,452 + LRK54_RS04375 0.10 +0.9 923,452 + LRK54_RS04375 0.10 -1.6 923,452 + LRK54_RS04375 0.10 -1.3 923,452 + LRK54_RS04375 0.10 -0.5 923,453 - LRK54_RS04375 0.10 +0.6 923,453 - LRK54_RS04375 0.10 +2.2 923,453 - LRK54_RS04375 0.10 -0.3 923,453 - LRK54_RS04375 0.10 -0.4 923,453 - LRK54_RS04375 0.10 -0.5 923,453 - LRK54_RS04375 0.10 -0.6 923,453 - LRK54_RS04375 0.10 -0.9 923,453 - LRK54_RS04375 0.10 -2.4 923,453 - LRK54_RS04375 0.10 +0.4 923,558 + LRK54_RS04375 0.34 +0.9 923,558 + LRK54_RS04375 0.34 +0.2 923,558 + LRK54_RS04375 0.34 -0.7 923,559 - LRK54_RS04375 0.34 -1.3 923,559 - LRK54_RS04375 0.34 -2.1 923,559 - LRK54_RS04375 0.34 -2.2 923,559 - LRK54_RS04375 0.34 -1.2 923,674 + LRK54_RS04375 0.60 -1.8 923,674 + LRK54_RS04375 0.60 +0.9 923,674 + LRK54_RS04375 0.60 +0.6 923,675 - LRK54_RS04375 0.60 +1.2 923,675 - LRK54_RS04375 0.60 -1.5 923,774 + LRK54_RS04375 0.82 -1.2 923,774 + LRK54_RS04375 0.82 -2.4 923,774 + LRK54_RS04375 0.82 -1.5 923,774 + LRK54_RS04375 0.82 -3.2 923,775 - LRK54_RS04375 0.82 +1.0 923,775 - LRK54_RS04375 0.82 -1.8 923,831 + -0.7 923,831 + -1.4 923,831 + -0.3 923,831 + -0.7 923,831 + +2.2 923,831 + -3.1 923,831 + +0.9 923,831 + -0.3 923,831 + -0.8 923,831 + -1.7 923,831 + +1.4 923,832 - -0.6 923,832 - +0.2 923,832 - -1.0 923,832 - +3.2 923,832 - +2.2 923,832 - -0.3 923,832 - -2.0 923,832 - +0.4 923,832 - -0.6 923,832 - -0.3 923,832 - +0.1 923,832 - +0.2 924,030 + LRK54_RS04380 0.18 +0.4 924,030 + LRK54_RS04380 0.18 +1.2 924,030 + LRK54_RS04380 0.18 -2.6 924,030 + LRK54_RS04380 0.18 -3.8 924,030 + LRK54_RS04380 0.18 +0.0 924,030 + LRK54_RS04380 0.18 -1.8 924,030 + LRK54_RS04380 0.18 +0.9 924,030 + LRK54_RS04380 0.18 +1.0 924,030 + LRK54_RS04380 0.18 +0.0 924,030 + LRK54_RS04380 0.18 +0.4 924,030 + LRK54_RS04380 0.18 +0.6 924,031 - LRK54_RS04380 0.18 -0.8 924,031 - LRK54_RS04380 0.18 -0.7 924,031 - LRK54_RS04380 0.18 -1.1 924,031 - LRK54_RS04380 0.18 -0.1 924,031 - LRK54_RS04380 0.18 +0.4 924,031 - LRK54_RS04380 0.18 +1.5 924,031 - LRK54_RS04380 0.18 -1.8 924,031 - LRK54_RS04380 0.18 +2.2 924,031 - LRK54_RS04380 0.18 -2.1 924,031 - LRK54_RS04380 0.18 +0.8 924,031 - LRK54_RS04380 0.18 +0.9 924,031 - LRK54_RS04380 0.18 -0.1 924,048 + LRK54_RS04380 0.19 +1.6 924,048 + LRK54_RS04380 0.19 -1.4 924,048 + LRK54_RS04380 0.19 -1.7 924,048 + LRK54_RS04380 0.19 -1.3 924,048 + LRK54_RS04380 0.19 -1.3 924,049 - LRK54_RS04380 0.20 +1.5 924,049 - LRK54_RS04380 0.20 +0.4 924,049 - LRK54_RS04380 0.20 +1.0 924,049 - LRK54_RS04380 0.20 +0.4 924,049 - LRK54_RS04380 0.20 -0.8 924,049 - LRK54_RS04380 0.20 +1.0 924,049 - LRK54_RS04380 0.20 +0.7 924,049 - LRK54_RS04380 0.20 +0.3 924,049 - LRK54_RS04380 0.20 -1.9 924,049 - LRK54_RS04380 0.20 +0.5 924,049 - LRK54_RS04380 0.20 -1.0
Or see this region's nucleotide sequence