Experiment: R2A with Chromium(III) Chloride Hexahydrate 0.09765625 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt LRK54_RS00905 and LRK54_RS00910 are separated by 69 nucleotides LRK54_RS00910 and LRK54_RS00915 are separated by 301 nucleotides
LRK54_RS00905: LRK54_RS00905 - cysteine synthase A, at 199,463 to 200,401
_RS00905
LRK54_RS00910: LRK54_RS00910 - siroheme synthase CysG, at 200,471 to 201,931
_RS00910
LRK54_RS00915: LRK54_RS00915 - LysR substrate-binding domain-containing protein, at 202,233 to 203,228
_RS00915
Position (kb) 200
201
202 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 199.515 kb on + strand at 199.515 kb on + strand at 199.515 kb on + strand at 199.515 kb on + strand at 199.515 kb on + strand at 199.515 kb on + strand at 199.515 kb on + strand at 199.515 kb on + strand at 199.516 kb on - strand at 199.516 kb on - strand at 199.893 kb on + strand, within LRK54_RS00905 at 199.893 kb on + strand, within LRK54_RS00905 at 199.893 kb on + strand, within LRK54_RS00905 at 199.893 kb on + strand, within LRK54_RS00905 at 199.893 kb on + strand, within LRK54_RS00905 at 199.893 kb on + strand, within LRK54_RS00905 at 199.894 kb on - strand, within LRK54_RS00905 at 199.894 kb on - strand, within LRK54_RS00905 at 199.894 kb on - strand, within LRK54_RS00905 at 199.894 kb on - strand, within LRK54_RS00905 at 199.894 kb on - strand, within LRK54_RS00905 at 199.894 kb on - strand, within LRK54_RS00905 at 199.953 kb on + strand, within LRK54_RS00905 at 199.953 kb on + strand, within LRK54_RS00905 at 199.954 kb on - strand, within LRK54_RS00905 at 199.954 kb on - strand, within LRK54_RS00905 at 199.954 kb on - strand, within LRK54_RS00905 at 199.954 kb on - strand, within LRK54_RS00905 at 199.954 kb on - strand, within LRK54_RS00905 at 200.082 kb on + strand, within LRK54_RS00905 at 200.119 kb on + strand, within LRK54_RS00905 at 200.119 kb on + strand, within LRK54_RS00905 at 200.119 kb on + strand, within LRK54_RS00905 at 200.120 kb on - strand, within LRK54_RS00905 at 200.120 kb on - strand, within LRK54_RS00905 at 200.120 kb on - strand, within LRK54_RS00905 at 200.316 kb on + strand at 200.316 kb on + strand at 200.316 kb on + strand at 200.316 kb on + strand at 200.316 kb on + strand at 200.316 kb on + strand at 200.316 kb on + strand at 200.316 kb on + strand at 200.316 kb on + strand at 200.316 kb on + strand at 200.316 kb on + strand at 200.316 kb on + strand at 200.316 kb on + strand at 200.316 kb on + strand at 200.316 kb on + strand at 200.316 kb on + strand at 200.316 kb on + strand at 200.316 kb on + strand at 200.316 kb on + strand at 200.316 kb on + strand at 200.316 kb on + strand at 200.316 kb on + strand at 200.316 kb on + strand at 200.316 kb on + strand at 200.317 kb on - strand at 200.317 kb on - strand at 200.317 kb on - strand at 200.317 kb on - strand at 200.317 kb on - strand at 200.317 kb on - strand at 200.317 kb on - strand at 200.317 kb on - strand at 200.317 kb on - strand at 200.317 kb on - strand at 200.317 kb on - strand at 200.317 kb on - strand at 200.317 kb on - strand at 200.317 kb on - strand at 200.317 kb on - strand at 200.317 kb on - strand at 200.317 kb on - strand at 200.317 kb on - strand at 200.317 kb on - strand at 200.317 kb on - strand at 200.534 kb on + strand at 200.787 kb on + strand, within LRK54_RS00910 at 200.787 kb on + strand, within LRK54_RS00910 at 200.787 kb on + strand, within LRK54_RS00910 at 200.787 kb on + strand, within LRK54_RS00910 at 200.787 kb on + strand, within LRK54_RS00910 at 200.787 kb on + strand, within LRK54_RS00910 at 200.787 kb on + strand, within LRK54_RS00910 at 200.787 kb on + strand, within LRK54_RS00910 at 200.787 kb on + strand, within LRK54_RS00910 at 200.787 kb on + strand, within LRK54_RS00910 at 200.787 kb on + strand, within LRK54_RS00910 at 200.787 kb on + strand, within LRK54_RS00910 at 200.787 kb on + strand, within LRK54_RS00910 at 200.787 kb on + strand, within LRK54_RS00910 at 200.787 kb on + strand, within LRK54_RS00910 at 200.787 kb on + strand, within LRK54_RS00910 at 200.788 kb on - strand, within LRK54_RS00910 at 200.788 kb on - strand, within LRK54_RS00910 at 200.788 kb on - strand, within LRK54_RS00910 at 200.788 kb on - strand, within LRK54_RS00910 at 200.788 kb on - strand, within LRK54_RS00910 at 200.788 kb on - strand, within LRK54_RS00910 at 200.788 kb on - strand, within LRK54_RS00910 at 200.788 kb on - strand, within LRK54_RS00910 at 200.788 kb on - strand, within LRK54_RS00910 at 200.788 kb on - strand, within LRK54_RS00910 at 200.788 kb on - strand, within LRK54_RS00910 at 200.788 kb on - strand, within LRK54_RS00910 at 200.788 kb on - strand, within LRK54_RS00910 at 200.788 kb on - strand, within LRK54_RS00910 at 200.788 kb on - strand, within LRK54_RS00910 at 200.788 kb on - strand, within LRK54_RS00910 at 200.788 kb on - strand, within LRK54_RS00910 at 200.788 kb on - strand, within LRK54_RS00910 at 200.788 kb on - strand, within LRK54_RS00910 at 200.788 kb on - strand, within LRK54_RS00910 at 200.788 kb on - strand, within LRK54_RS00910 at 200.961 kb on + strand, within LRK54_RS00910 at 200.961 kb on + strand, within LRK54_RS00910 at 200.961 kb on + strand, within LRK54_RS00910 at 200.961 kb on + strand, within LRK54_RS00910 at 200.962 kb on - strand, within LRK54_RS00910 at 200.962 kb on - strand, within LRK54_RS00910 at 200.962 kb on - strand, within LRK54_RS00910 at 201.921 kb on + strand at 201.921 kb on + strand at 201.921 kb on + strand at 201.921 kb on + strand at 201.921 kb on + strand at 201.922 kb on - strand at 201.922 kb on - strand at 201.922 kb on - strand at 201.922 kb on - strand at 201.922 kb on - strand at 201.922 kb on - strand at 201.975 kb on - strand at 201.975 kb on - strand at 202.019 kb on + strand at 202.019 kb on + strand at 202.019 kb on + strand at 202.019 kb on + strand at 202.020 kb on - strand at 202.020 kb on - strand at 202.020 kb on - strand at 202.036 kb on + strand at 202.036 kb on + strand at 202.036 kb on + strand at 202.036 kb on + strand at 202.036 kb on + strand at 202.037 kb on - strand at 202.037 kb on - strand at 202.037 kb on - strand at 202.037 kb on - strand at 202.037 kb on - strand at 202.077 kb on - strand at 202.077 kb on - strand at 202.077 kb on - strand at 202.082 kb on + strand at 202.082 kb on + strand at 202.082 kb on + strand at 202.082 kb on + strand at 202.082 kb on + strand at 202.082 kb on + strand at 202.082 kb on + strand at 202.082 kb on + strand at 202.083 kb on - strand at 202.083 kb on - strand at 202.083 kb on - strand at 202.084 kb on + strand at 202.084 kb on + strand at 202.084 kb on + strand at 202.084 kb on + strand at 202.085 kb on - strand at 202.085 kb on - strand at 202.085 kb on - strand at 202.085 kb on - strand at 202.094 kb on + strand at 202.094 kb on + strand at 202.174 kb on + strand at 202.174 kb on + strand at 202.174 kb on + strand at 202.174 kb on + strand at 202.175 kb on - strand at 202.175 kb on - strand at 202.175 kb on - strand at 202.175 kb on - strand at 202.175 kb on - strand at 202.175 kb on - strand at 202.175 kb on - strand at 202.175 kb on - strand at 202.175 kb on - strand at 202.175 kb on - strand at 202.254 kb on + strand at 202.722 kb on + strand, within LRK54_RS00915 at 202.723 kb on - strand, within LRK54_RS00915 at 202.723 kb on - strand, within LRK54_RS00915 at 202.824 kb on + strand, within LRK54_RS00915 at 202.824 kb on + strand, within LRK54_RS00915 at 202.824 kb on + strand, within LRK54_RS00915 at 202.824 kb on + strand, within LRK54_RS00915 at 202.824 kb on + strand, within LRK54_RS00915 at 202.825 kb on - strand, within LRK54_RS00915 at 202.825 kb on - strand, within LRK54_RS00915 at 202.825 kb on - strand, within LRK54_RS00915
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Chromium(III) Chloride Hexahydrate 0.09765625 mM remove 199,515 + +0.3 199,515 + +0.8 199,515 + -2.0 199,515 + +0.8 199,515 + -0.8 199,515 + -0.3 199,515 + -0.2 199,515 + -2.6 199,516 - +1.1 199,516 - -1.7 199,893 + LRK54_RS00905 0.46 -1.3 199,893 + LRK54_RS00905 0.46 -0.5 199,893 + LRK54_RS00905 0.46 -2.8 199,893 + LRK54_RS00905 0.46 -0.0 199,893 + LRK54_RS00905 0.46 +0.8 199,893 + LRK54_RS00905 0.46 -2.0 199,894 - LRK54_RS00905 0.46 +0.5 199,894 - LRK54_RS00905 0.46 -2.2 199,894 - LRK54_RS00905 0.46 +2.1 199,894 - LRK54_RS00905 0.46 -0.9 199,894 - LRK54_RS00905 0.46 -2.9 199,894 - LRK54_RS00905 0.46 -0.6 199,953 + LRK54_RS00905 0.52 -0.2 199,953 + LRK54_RS00905 0.52 -0.0 199,954 - LRK54_RS00905 0.52 -0.6 199,954 - LRK54_RS00905 0.52 -2.0 199,954 - LRK54_RS00905 0.52 +3.1 199,954 - LRK54_RS00905 0.52 -0.2 199,954 - LRK54_RS00905 0.52 +1.5 200,082 + LRK54_RS00905 0.66 -1.3 200,119 + LRK54_RS00905 0.70 -1.8 200,119 + LRK54_RS00905 0.70 +1.3 200,119 + LRK54_RS00905 0.70 -0.2 200,120 - LRK54_RS00905 0.70 -1.6 200,120 - LRK54_RS00905 0.70 -0.2 200,120 - LRK54_RS00905 0.70 -0.6 200,316 + +0.8 200,316 + -1.1 200,316 + -0.0 200,316 + +3.1 200,316 + +0.1 200,316 + +0.2 200,316 + +0.1 200,316 + -1.5 200,316 + +2.1 200,316 + +0.3 200,316 + +1.1 200,316 + -1.2 200,316 + +1.5 200,316 + +0.6 200,316 + -0.8 200,316 + +0.3 200,316 + -0.0 200,316 + -2.1 200,316 + -0.3 200,316 + +0.0 200,316 + +0.2 200,316 + -0.5 200,316 + +1.5 200,316 + +0.8 200,317 - -0.3 200,317 - -1.5 200,317 - +0.6 200,317 - -1.0 200,317 - +0.8 200,317 - +1.5 200,317 - +1.5 200,317 - +0.9 200,317 - -0.5 200,317 - -0.6 200,317 - +0.2 200,317 - +0.5 200,317 - -2.1 200,317 - +1.5 200,317 - -2.6 200,317 - +1.5 200,317 - -0.5 200,317 - +0.3 200,317 - +0.1 200,317 - -2.6 200,534 + +1.1 200,787 + LRK54_RS00910 0.22 +1.5 200,787 + LRK54_RS00910 0.22 -1.1 200,787 + LRK54_RS00910 0.22 -1.0 200,787 + LRK54_RS00910 0.22 +0.3 200,787 + LRK54_RS00910 0.22 -1.9 200,787 + LRK54_RS00910 0.22 +0.3 200,787 + LRK54_RS00910 0.22 -0.2 200,787 + LRK54_RS00910 0.22 -0.6 200,787 + LRK54_RS00910 0.22 -1.5 200,787 + LRK54_RS00910 0.22 +2.1 200,787 + LRK54_RS00910 0.22 +0.3 200,787 + LRK54_RS00910 0.22 -1.5 200,787 + LRK54_RS00910 0.22 -1.3 200,787 + LRK54_RS00910 0.22 +0.7 200,787 + LRK54_RS00910 0.22 -1.9 200,787 + LRK54_RS00910 0.22 -1.1 200,788 - LRK54_RS00910 0.22 -1.0 200,788 - LRK54_RS00910 0.22 -1.3 200,788 - LRK54_RS00910 0.22 -0.2 200,788 - LRK54_RS00910 0.22 -0.0 200,788 - LRK54_RS00910 0.22 -1.0 200,788 - LRK54_RS00910 0.22 -0.8 200,788 - LRK54_RS00910 0.22 -1.9 200,788 - LRK54_RS00910 0.22 -2.6 200,788 - LRK54_RS00910 0.22 -2.6 200,788 - LRK54_RS00910 0.22 -2.3 200,788 - LRK54_RS00910 0.22 -1.5 200,788 - LRK54_RS00910 0.22 +0.7 200,788 - LRK54_RS00910 0.22 -1.0 200,788 - LRK54_RS00910 0.22 +1.5 200,788 - LRK54_RS00910 0.22 -1.9 200,788 - LRK54_RS00910 0.22 -0.4 200,788 - LRK54_RS00910 0.22 +0.6 200,788 - LRK54_RS00910 0.22 +0.3 200,788 - LRK54_RS00910 0.22 -1.4 200,788 - LRK54_RS00910 0.22 -0.0 200,788 - LRK54_RS00910 0.22 -0.6 200,961 + LRK54_RS00910 0.34 -1.1 200,961 + LRK54_RS00910 0.34 +1.1 200,961 + LRK54_RS00910 0.34 -1.9 200,961 + LRK54_RS00910 0.34 -1.1 200,962 - LRK54_RS00910 0.34 -2.0 200,962 - LRK54_RS00910 0.34 -1.3 200,962 - LRK54_RS00910 0.34 +2.1 201,921 + +1.5 201,921 + -1.0 201,921 + -1.6 201,921 + -2.9 201,921 + -0.5 201,922 - -0.2 201,922 - -0.0 201,922 - +1.5 201,922 - +0.5 201,922 - -0.3 201,922 - -1.8 201,975 - -1.3 201,975 - +0.4 202,019 + +0.5 202,019 + +0.4 202,019 + +0.3 202,019 + +0.1 202,020 - +1.5 202,020 - -0.8 202,020 - -0.6 202,036 + -1.0 202,036 + -0.5 202,036 + -2.1 202,036 + -0.7 202,036 + -3.7 202,037 - -1.9 202,037 - -1.2 202,037 - -3.2 202,037 - -1.3 202,037 - +0.1 202,077 - -1.7 202,077 - -0.8 202,077 - +1.1 202,082 + -0.8 202,082 + +0.3 202,082 + -2.1 202,082 + +0.8 202,082 + -2.0 202,082 + -0.0 202,082 + -1.5 202,082 + +0.3 202,083 - -1.7 202,083 - +0.6 202,083 - -1.4 202,084 + -2.1 202,084 + -2.5 202,084 + -0.6 202,084 + -2.9 202,085 - +0.3 202,085 - -1.0 202,085 - -0.3 202,085 - -1.3 202,094 + +0.8 202,094 + +0.1 202,174 + +1.6 202,174 + -0.5 202,174 + -0.7 202,174 + +0.1 202,175 - -0.9 202,175 - -0.1 202,175 - -1.6 202,175 - -2.4 202,175 - -0.7 202,175 - -0.5 202,175 - -0.8 202,175 - -0.8 202,175 - -1.0 202,175 - -1.0 202,254 + -0.0 202,722 + LRK54_RS00915 0.49 +0.1 202,723 - LRK54_RS00915 0.49 +2.1 202,723 - LRK54_RS00915 0.49 -0.2 202,824 + LRK54_RS00915 0.59 -0.4 202,824 + LRK54_RS00915 0.59 +0.5 202,824 + LRK54_RS00915 0.59 -0.9 202,824 + LRK54_RS00915 0.59 -0.6 202,824 + LRK54_RS00915 0.59 -1.3 202,825 - LRK54_RS00915 0.59 -0.0 202,825 - LRK54_RS00915 0.59 +0.8 202,825 - LRK54_RS00915 0.59 -1.0
Or see this region's nucleotide sequence