Experiment: MoLS4 with L-Glutamine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ruvC and DVU2259 are separated by 74 nucleotides DVU2259 and rrmJ are separated by 53 nucleotides rrmJ and DVU2261 are separated by 55 nucleotides
DVU2258: ruvC - crossover junction endodeoxyribonuclease RuvC (TIGR), at 2,352,212 to 2,352,712
ruvC
DVU2259: DVU2259 - conserved hypothetical protein TIGR01033 (TIGR), at 2,352,787 to 2,353,530
DVU2259
DVU2260: rrmJ - ribosomal RNA large subunit methyltransferase J (TIGR), at 2,353,584 to 2,354,198
rrmJ
DVU2261: DVU2261 - hypothetical protein (TIGR), at 2,354,254 to 2,356,068
DVU2261
Position (kb)
2353
2354
2355 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 2352.653 kb on + strand, within ruvC at 2352.668 kb on - strand at 2352.775 kb on + strand at 2352.785 kb on + strand at 2352.793 kb on - strand at 2352.968 kb on + strand, within DVU2259 at 2352.976 kb on - strand, within DVU2259 at 2353.032 kb on - strand, within DVU2259 at 2353.032 kb on - strand, within DVU2259 at 2353.058 kb on + strand, within DVU2259 at 2353.081 kb on - strand, within DVU2259 at 2353.091 kb on - strand, within DVU2259 at 2353.208 kb on + strand, within DVU2259 at 2353.342 kb on + strand, within DVU2259 at 2353.432 kb on + strand, within DVU2259 at 2353.529 kb on - strand at 2353.561 kb on + strand at 2353.610 kb on - strand at 2353.619 kb on + strand at 2353.735 kb on - strand, within rrmJ at 2353.807 kb on - strand, within rrmJ at 2353.829 kb on + strand, within rrmJ at 2353.919 kb on + strand, within rrmJ at 2353.927 kb on - strand, within rrmJ at 2353.946 kb on + strand, within rrmJ at 2353.985 kb on + strand, within rrmJ at 2354.215 kb on + strand at 2354.405 kb on - strand at 2354.430 kb on + strand at 2354.438 kb on - strand, within DVU2261 at 2354.460 kb on + strand, within DVU2261 at 2354.463 kb on - strand, within DVU2261 at 2354.504 kb on + strand, within DVU2261 at 2354.562 kb on - strand, within DVU2261 at 2354.569 kb on - strand, within DVU2261 at 2354.573 kb on + strand, within DVU2261 at 2354.607 kb on + strand, within DVU2261 at 2354.614 kb on + strand, within DVU2261 at 2354.626 kb on + strand, within DVU2261 at 2354.654 kb on - strand, within DVU2261 at 2354.693 kb on - strand, within DVU2261 at 2354.718 kb on - strand, within DVU2261 at 2354.718 kb on - strand, within DVU2261 at 2354.773 kb on + strand, within DVU2261 at 2354.865 kb on + strand, within DVU2261 at 2354.865 kb on + strand, within DVU2261 at 2354.865 kb on + strand, within DVU2261 at 2354.873 kb on - strand, within DVU2261 at 2354.873 kb on - strand, within DVU2261 at 2354.896 kb on + strand, within DVU2261 at 2354.904 kb on - strand, within DVU2261 at 2354.904 kb on - strand, within DVU2261 at 2354.904 kb on - strand, within DVU2261 at 2354.966 kb on - strand, within DVU2261 at 2354.971 kb on - strand, within DVU2261 at 2354.993 kb on - strand, within DVU2261 at 2355.086 kb on + strand, within DVU2261 at 2355.094 kb on - strand, within DVU2261 at 2355.117 kb on + strand, within DVU2261 at 2355.123 kb on + strand, within DVU2261 at 2355.142 kb on + strand, within DVU2261 at 2355.142 kb on + strand, within DVU2261 at 2355.142 kb on + strand, within DVU2261 at 2355.150 kb on - strand, within DVU2261 at 2355.150 kb on - strand, within DVU2261 at 2355.150 kb on - strand, within DVU2261 at 2355.150 kb on - strand, within DVU2261 at 2355.174 kb on + strand, within DVU2261 at 2355.181 kb on + strand, within DVU2261 at 2355.181 kb on + strand, within DVU2261
Per-strain Table
Position Strand Gene LocusTag Fraction MoLS4 with L-Glutamine (N) remove 2,352,653 + ruvC DVU2258 0.88 -0.2 2,352,668 - -0.7 2,352,775 + -3.1 2,352,785 + +0.5 2,352,793 - -0.4 2,352,968 + DVU2259 0.24 -0.8 2,352,976 - DVU2259 0.25 -0.4 2,353,032 - DVU2259 0.33 -0.0 2,353,032 - DVU2259 0.33 +0.2 2,353,058 + DVU2259 0.36 +0.2 2,353,081 - DVU2259 0.40 +0.0 2,353,091 - DVU2259 0.41 -0.4 2,353,208 + DVU2259 0.57 -0.0 2,353,342 + DVU2259 0.75 -1.9 2,353,432 + DVU2259 0.87 +0.0 2,353,529 - -1.2 2,353,561 + -0.6 2,353,610 - -3.1 2,353,619 + -0.2 2,353,735 - rrmJ DVU2260 0.25 -3.2 2,353,807 - rrmJ DVU2260 0.36 -0.3 2,353,829 + rrmJ DVU2260 0.40 -0.2 2,353,919 + rrmJ DVU2260 0.54 -1.2 2,353,927 - rrmJ DVU2260 0.56 -3.3 2,353,946 + rrmJ DVU2260 0.59 +0.5 2,353,985 + rrmJ DVU2260 0.65 -0.2 2,354,215 + -0.5 2,354,405 - -0.2 2,354,430 + +0.5 2,354,438 - DVU2261 0.10 +0.2 2,354,460 + DVU2261 0.11 -0.5 2,354,463 - DVU2261 0.12 +0.3 2,354,504 + DVU2261 0.14 -0.7 2,354,562 - DVU2261 0.17 -1.8 2,354,569 - DVU2261 0.17 -0.3 2,354,573 + DVU2261 0.18 -0.6 2,354,607 + DVU2261 0.19 -0.3 2,354,614 + DVU2261 0.20 +1.1 2,354,626 + DVU2261 0.20 -0.1 2,354,654 - DVU2261 0.22 +0.9 2,354,693 - DVU2261 0.24 -0.1 2,354,718 - DVU2261 0.26 -0.0 2,354,718 - DVU2261 0.26 +0.2 2,354,773 + DVU2261 0.29 +1.0 2,354,865 + DVU2261 0.34 +0.2 2,354,865 + DVU2261 0.34 -0.1 2,354,865 + DVU2261 0.34 -0.2 2,354,873 - DVU2261 0.34 -0.3 2,354,873 - DVU2261 0.34 +0.2 2,354,896 + DVU2261 0.35 -3.0 2,354,904 - DVU2261 0.36 -0.1 2,354,904 - DVU2261 0.36 -0.2 2,354,904 - DVU2261 0.36 +0.1 2,354,966 - DVU2261 0.39 +0.2 2,354,971 - DVU2261 0.40 -0.2 2,354,993 - DVU2261 0.41 +0.7 2,355,086 + DVU2261 0.46 -0.7 2,355,094 - DVU2261 0.46 +1.1 2,355,117 + DVU2261 0.48 -0.6 2,355,123 + DVU2261 0.48 -0.8 2,355,142 + DVU2261 0.49 +1.8 2,355,142 + DVU2261 0.49 -3.1 2,355,142 + DVU2261 0.49 -0.1 2,355,150 - DVU2261 0.49 -0.2 2,355,150 - DVU2261 0.49 +0.6 2,355,150 - DVU2261 0.49 -0.3 2,355,150 - DVU2261 0.49 -0.2 2,355,174 + DVU2261 0.51 -0.2 2,355,181 + DVU2261 0.51 +0.8 2,355,181 + DVU2261 0.51 +0.1
Or see this region's nucleotide sequence