Experiment: MoLS4 with L-Glutamine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt DVU1983 and msrA are separated by 127 nucleotides msrA and DVU1985 are separated by 109 nucleotides
DVU1983: DVU1983 - hypothetical protein (TIGR), at 2,060,785 to 2,062,008
DVU1983
DVU1984: msrA - peptide methionine sulfoxide reductase MsrA (TIGR), at 2,062,136 to 2,062,762
msrA
DVU1985: DVU1985 - conserved domain protein (TIGR), at 2,062,872 to 2,064,128
DVU1985
Position (kb)
2062
2063 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 2061.142 kb on + strand, within DVU1983 at 2061.181 kb on - strand, within DVU1983 at 2061.294 kb on + strand, within DVU1983 at 2061.309 kb on + strand, within DVU1983 at 2061.332 kb on - strand, within DVU1983 at 2061.337 kb on - strand, within DVU1983 at 2061.395 kb on + strand, within DVU1983 at 2061.444 kb on + strand, within DVU1983 at 2061.463 kb on + strand, within DVU1983 at 2061.511 kb on + strand, within DVU1983 at 2061.612 kb on + strand, within DVU1983 at 2061.715 kb on + strand, within DVU1983 at 2061.717 kb on + strand, within DVU1983 at 2061.723 kb on - strand, within DVU1983 at 2061.762 kb on + strand, within DVU1983 at 2061.764 kb on + strand, within DVU1983 at 2061.764 kb on + strand, within DVU1983 at 2061.764 kb on + strand, within DVU1983 at 2061.770 kb on - strand, within DVU1983 at 2061.772 kb on - strand, within DVU1983 at 2061.772 kb on - strand, within DVU1983 at 2061.772 kb on - strand, within DVU1983 at 2061.772 kb on - strand, within DVU1983 at 2061.772 kb on - strand, within DVU1983 at 2061.796 kb on + strand, within DVU1983 at 2061.808 kb on + strand, within DVU1983 at 2061.810 kb on + strand, within DVU1983 at 2061.810 kb on + strand, within DVU1983 at 2061.816 kb on - strand, within DVU1983 at 2061.816 kb on - strand, within DVU1983 at 2061.955 kb on + strand at 2061.995 kb on - strand at 2062.008 kb on + strand at 2062.081 kb on - strand at 2062.175 kb on - strand at 2062.325 kb on + strand, within msrA at 2062.423 kb on - strand, within msrA at 2062.583 kb on - strand, within msrA at 2062.592 kb on - strand, within msrA at 2062.639 kb on + strand, within msrA at 2062.661 kb on + strand, within msrA at 2062.666 kb on + strand, within msrA at 2062.742 kb on + strand at 2062.752 kb on + strand at 2062.752 kb on + strand at 2062.908 kb on + strand at 2062.928 kb on + strand at 2062.965 kb on + strand at 2063.014 kb on - strand, within DVU1985 at 2063.075 kb on - strand, within DVU1985 at 2063.379 kb on - strand, within DVU1985
Per-strain Table
Position Strand Gene LocusTag Fraction MoLS4 with L-Glutamine (N) remove 2,061,142 + DVU1983 0.29 +0.0 2,061,181 - DVU1983 0.32 -0.8 2,061,294 + DVU1983 0.42 -0.2 2,061,309 + DVU1983 0.43 -0.5 2,061,332 - DVU1983 0.45 -1.2 2,061,337 - DVU1983 0.45 -0.1 2,061,395 + DVU1983 0.50 -0.3 2,061,444 + DVU1983 0.54 -3.4 2,061,463 + DVU1983 0.55 +0.3 2,061,511 + DVU1983 0.59 +0.8 2,061,612 + DVU1983 0.68 -0.7 2,061,715 + DVU1983 0.76 -0.1 2,061,717 + DVU1983 0.76 -0.3 2,061,723 - DVU1983 0.77 -0.3 2,061,762 + DVU1983 0.80 -0.8 2,061,764 + DVU1983 0.80 +1.3 2,061,764 + DVU1983 0.80 -0.4 2,061,764 + DVU1983 0.80 -0.7 2,061,770 - DVU1983 0.80 -0.5 2,061,772 - DVU1983 0.81 +0.1 2,061,772 - DVU1983 0.81 +0.6 2,061,772 - DVU1983 0.81 +0.6 2,061,772 - DVU1983 0.81 -1.0 2,061,772 - DVU1983 0.81 +0.0 2,061,796 + DVU1983 0.83 -0.0 2,061,808 + DVU1983 0.84 -0.1 2,061,810 + DVU1983 0.84 -4.0 2,061,810 + DVU1983 0.84 -2.3 2,061,816 - DVU1983 0.84 -2.9 2,061,816 - DVU1983 0.84 -3.0 2,061,955 + -0.1 2,061,995 - -3.7 2,062,008 + -0.3 2,062,081 - +1.6 2,062,175 - +0.8 2,062,325 + msrA DVU1984 0.30 +0.0 2,062,423 - msrA DVU1984 0.46 -3.3 2,062,583 - msrA DVU1984 0.71 -0.5 2,062,592 - msrA DVU1984 0.73 +0.2 2,062,639 + msrA DVU1984 0.80 -2.9 2,062,661 + msrA DVU1984 0.84 -0.3 2,062,666 + msrA DVU1984 0.85 -3.2 2,062,742 + -1.5 2,062,752 + +0.1 2,062,752 + -0.8 2,062,908 + -0.7 2,062,928 + +0.1 2,062,965 + +0.4 2,063,014 - DVU1985 0.11 +0.2 2,063,075 - DVU1985 0.16 +0.3 2,063,379 - DVU1985 0.40 -0.3
Or see this region's nucleotide sequence