Experiment: MoLS4 with L-Glutamine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt rfbA and rfbC overlap by 4 nucleotides rfbC and DVU0699 are separated by 176 nucleotides DVU0699 and tRNA-Ser are separated by 56 nucleotides tRNA-Ser and DVU0700 are separated by 264 nucleotides
DVU0697: rfbA - mannose-1-phosphate guanylyltransferase/mannose-6-phosphate isomerase (TIGR), at 771,808 to 773,226
rfbA
DVU0698: rfbC - dTDP-4-dehydrorhamnose 3,5-epimerase (TIGR), at 773,223 to 773,795
rfbC
DVU0699: DVU0699 - sensory box sensor histidine kinase/response regulator, authentic frameshift (TIGR), at 773,972 to 775,650
DVU0699
DVU_tRNA-Ser-1: tRNA-Ser - tRNA with anticodon TGA for Ser (VIMSS-AUTO), at 775,707 to 775,798
tRNA-Ser
DVU0700: DVU0700 - methyl-accepting chemotaxis protein (TIGR), at 776,063 to 778,519
DVU0700
Position (kb) 773
774
775
776 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 773.386 kb on - strand, within rfbC at 773.395 kb on + strand, within rfbC at 773.482 kb on - strand, within rfbC at 773.497 kb on - strand, within rfbC at 773.542 kb on - strand, within rfbC at 773.572 kb on - strand, within rfbC at 773.577 kb on - strand, within rfbC at 773.878 kb on - strand at 773.882 kb on + strand at 773.981 kb on + strand at 773.983 kb on + strand at 774.054 kb on - strand at 774.054 kb on - strand at 774.060 kb on - strand at 774.068 kb on + strand at 774.123 kb on + strand at 774.123 kb on + strand at 774.131 kb on - strand at 774.131 kb on - strand at 774.135 kb on + strand at 774.151 kb on + strand, within DVU0699 at 774.158 kb on + strand, within DVU0699 at 774.208 kb on + strand, within DVU0699 at 774.286 kb on + strand, within DVU0699 at 774.323 kb on + strand, within DVU0699 at 774.375 kb on - strand, within DVU0699 at 774.460 kb on + strand, within DVU0699 at 774.540 kb on + strand, within DVU0699 at 774.541 kb on - strand, within DVU0699 at 774.564 kb on + strand, within DVU0699 at 774.567 kb on - strand, within DVU0699 at 774.567 kb on - strand, within DVU0699 at 774.593 kb on + strand, within DVU0699 at 774.685 kb on + strand, within DVU0699 at 774.706 kb on + strand, within DVU0699 at 774.706 kb on + strand, within DVU0699 at 774.716 kb on - strand, within DVU0699 at 774.844 kb on + strand, within DVU0699 at 774.844 kb on + strand, within DVU0699 at 774.877 kb on + strand, within DVU0699 at 774.877 kb on + strand, within DVU0699 at 774.905 kb on + strand, within DVU0699 at 775.016 kb on + strand, within DVU0699 at 775.021 kb on + strand, within DVU0699 at 775.058 kb on + strand, within DVU0699 at 775.058 kb on + strand, within DVU0699 at 775.058 kb on + strand, within DVU0699 at 775.058 kb on + strand, within DVU0699 at 775.058 kb on + strand, within DVU0699 at 775.058 kb on + strand, within DVU0699 at 775.059 kb on + strand, within DVU0699 at 775.066 kb on - strand, within DVU0699 at 775.066 kb on - strand, within DVU0699 at 775.078 kb on - strand, within DVU0699 at 775.182 kb on - strand, within DVU0699 at 775.257 kb on + strand, within DVU0699 at 775.312 kb on + strand, within DVU0699 at 775.313 kb on - strand, within DVU0699 at 775.313 kb on - strand, within DVU0699 at 775.373 kb on + strand, within DVU0699 at 775.381 kb on - strand, within DVU0699 at 775.402 kb on + strand, within DVU0699 at 775.516 kb on - strand at 775.705 kb on + strand at 776.069 kb on - strand at 776.113 kb on + strand at 776.115 kb on - strand at 776.123 kb on + strand at 776.123 kb on + strand at 776.131 kb on - strand at 776.167 kb on - strand at 776.170 kb on + strand at 776.384 kb on - strand, within DVU0700 at 776.402 kb on + strand, within DVU0700 at 776.412 kb on + strand, within DVU0700 at 776.475 kb on - strand, within DVU0700 at 776.574 kb on + strand, within DVU0700 at 776.587 kb on - strand, within DVU0700 at 776.632 kb on - strand, within DVU0700 at 776.632 kb on - strand, within DVU0700 at 776.634 kb on + strand, within DVU0700 at 776.635 kb on + strand, within DVU0700 at 776.645 kb on + strand, within DVU0700
Per-strain Table
Position Strand Gene LocusTag Fraction MoLS4 with L-Glutamine (N) remove 773,386 - rfbC DVU0698 0.28 +0.2 773,395 + rfbC DVU0698 0.30 -3.4 773,482 - rfbC DVU0698 0.45 +0.2 773,497 - rfbC DVU0698 0.48 +0.2 773,542 - rfbC DVU0698 0.56 -2.0 773,572 - rfbC DVU0698 0.61 -0.0 773,577 - rfbC DVU0698 0.62 -0.3 773,878 - -3.1 773,882 + -0.4 773,981 + +0.1 773,983 + +0.5 774,054 - +0.5 774,054 - +0.4 774,060 - -0.7 774,068 + +0.1 774,123 + -0.1 774,123 + +0.1 774,131 - +0.1 774,131 - -3.0 774,135 + -0.1 774,151 + DVU0699 0.11 -0.2 774,158 + DVU0699 0.11 -0.6 774,208 + DVU0699 0.14 -3.2 774,286 + DVU0699 0.19 -0.3 774,323 + DVU0699 0.21 -0.4 774,375 - DVU0699 0.24 +0.1 774,460 + DVU0699 0.29 -1.7 774,540 + DVU0699 0.34 +0.1 774,541 - DVU0699 0.34 -0.0 774,564 + DVU0699 0.35 +0.4 774,567 - DVU0699 0.35 -0.3 774,567 - DVU0699 0.35 +0.4 774,593 + DVU0699 0.37 -0.6 774,685 + DVU0699 0.42 +0.8 774,706 + DVU0699 0.44 -0.3 774,706 + DVU0699 0.44 -0.3 774,716 - DVU0699 0.44 -1.8 774,844 + DVU0699 0.52 +0.6 774,844 + DVU0699 0.52 -0.3 774,877 + DVU0699 0.54 -3.1 774,877 + DVU0699 0.54 +0.1 774,905 + DVU0699 0.56 +1.2 775,016 + DVU0699 0.62 +0.3 775,021 + DVU0699 0.62 -2.8 775,058 + DVU0699 0.65 -3.2 775,058 + DVU0699 0.65 -0.4 775,058 + DVU0699 0.65 -0.4 775,058 + DVU0699 0.65 -2.9 775,058 + DVU0699 0.65 -0.4 775,058 + DVU0699 0.65 -0.7 775,059 + DVU0699 0.65 -3.2 775,066 - DVU0699 0.65 -0.2 775,066 - DVU0699 0.65 +0.1 775,078 - DVU0699 0.66 -0.2 775,182 - DVU0699 0.72 +0.2 775,257 + DVU0699 0.77 -0.1 775,312 + DVU0699 0.80 -0.3 775,313 - DVU0699 0.80 +0.4 775,313 - DVU0699 0.80 -0.4 775,373 + DVU0699 0.83 +1.7 775,381 - DVU0699 0.84 -0.0 775,402 + DVU0699 0.85 -1.8 775,516 - -2.7 775,705 + -0.3 776,069 - -0.7 776,113 + -0.1 776,115 - -0.4 776,123 + +0.1 776,123 + -2.6 776,131 - +0.1 776,167 - -0.3 776,170 + -0.1 776,384 - DVU0700 0.13 -0.6 776,402 + DVU0700 0.14 -3.2 776,412 + DVU0700 0.14 +0.1 776,475 - DVU0700 0.17 +1.1 776,574 + DVU0700 0.21 -0.3 776,587 - DVU0700 0.21 -0.4 776,632 - DVU0700 0.23 -0.6 776,632 - DVU0700 0.23 +0.0 776,634 + DVU0700 0.23 +0.4 776,635 + DVU0700 0.23 -0.4 776,645 + DVU0700 0.24 -0.9
Or see this region's nucleotide sequence