Experiment: L-Malic Acid (C) with ammonium chloride; high light
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Rru_A1297 and Rru_A1298 overlap by 1 nucleotides Rru_A1298 and Rru_A1299 are separated by 20 nucleotides Rru_A1299 and Rru_A1300 are separated by 5 nucleotides Rru_A1300 and Rru_A1301 are separated by 16 nucleotides
Rru_A1297: Rru_A1297 - Amino acid ABC transporter, permease protein, 3-TM region, His/Glu/Gln/Arg/opine (NCBI), at 1,530,813 to 1,531,496
_A1297
Rru_A1298: Rru_A1298 - ABC transporter component (NCBI), at 1,531,496 to 1,532,287
_A1298
Rru_A1299: Rru_A1299 - FAD linked oxidase-like (NCBI), at 1,532,308 to 1,533,678
_A1299
Rru_A1300: Rru_A1300 - N-formylglutamate deformylase (NCBI), at 1,533,684 to 1,534,478
_A1300
Rru_A1301: Rru_A1301 - Histidine ammonia-lyase (NCBI), at 1,534,495 to 1,536,039
_A1301
Position (kb)
1532
1533
1534 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 1531.329 kb on + strand, within Rru_A1297 at 1531.329 kb on + strand, within Rru_A1297 at 1531.330 kb on - strand, within Rru_A1297 at 1531.399 kb on - strand, within Rru_A1297 at 1531.660 kb on + strand, within Rru_A1298 at 1531.661 kb on - strand, within Rru_A1298 at 1531.661 kb on - strand, within Rru_A1298 at 1531.680 kb on - strand, within Rru_A1298 at 1531.822 kb on + strand, within Rru_A1298 at 1531.822 kb on + strand, within Rru_A1298 at 1531.822 kb on + strand, within Rru_A1298 at 1531.822 kb on + strand, within Rru_A1298 at 1531.822 kb on + strand, within Rru_A1298 at 1531.823 kb on - strand, within Rru_A1298 at 1532.265 kb on - strand at 1532.349 kb on - strand at 1532.578 kb on - strand, within Rru_A1299 at 1532.578 kb on - strand, within Rru_A1299 at 1532.619 kb on + strand, within Rru_A1299 at 1532.619 kb on + strand, within Rru_A1299 at 1532.620 kb on - strand, within Rru_A1299 at 1533.092 kb on - strand, within Rru_A1299 at 1533.277 kb on + strand, within Rru_A1299 at 1533.278 kb on - strand, within Rru_A1299 at 1533.676 kb on + strand at 1533.685 kb on + strand at 1533.686 kb on - strand at 1533.686 kb on - strand at 1533.686 kb on - strand at 1533.766 kb on + strand, within Rru_A1300 at 1533.766 kb on + strand, within Rru_A1300 at 1533.767 kb on - strand, within Rru_A1300 at 1533.782 kb on + strand, within Rru_A1300 at 1533.783 kb on - strand, within Rru_A1300 at 1533.783 kb on - strand, within Rru_A1300 at 1533.784 kb on + strand, within Rru_A1300 at 1533.784 kb on + strand, within Rru_A1300 at 1533.785 kb on - strand, within Rru_A1300 at 1533.951 kb on + strand, within Rru_A1300 at 1534.043 kb on - strand, within Rru_A1300 at 1534.172 kb on - strand, within Rru_A1300 at 1534.466 kb on - strand at 1534.466 kb on - strand at 1534.466 kb on - strand at 1534.466 kb on - strand at 1534.592 kb on + strand at 1534.592 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Malic Acid (C) with ammonium chloride; high light remove 1,531,329 + Rru_A1297 0.75 -0.2 1,531,329 + Rru_A1297 0.75 -0.2 1,531,330 - Rru_A1297 0.76 -0.2 1,531,399 - Rru_A1297 0.86 -0.2 1,531,660 + Rru_A1298 0.21 -1.2 1,531,661 - Rru_A1298 0.21 -0.6 1,531,661 - Rru_A1298 0.21 -0.2 1,531,680 - Rru_A1298 0.23 -0.2 1,531,822 + Rru_A1298 0.41 -0.2 1,531,822 + Rru_A1298 0.41 -0.2 1,531,822 + Rru_A1298 0.41 -0.2 1,531,822 + Rru_A1298 0.41 -0.2 1,531,822 + Rru_A1298 0.41 -1.8 1,531,823 - Rru_A1298 0.41 -2.2 1,532,265 - -1.2 1,532,349 - -0.2 1,532,578 - Rru_A1299 0.20 -0.2 1,532,578 - Rru_A1299 0.20 -0.2 1,532,619 + Rru_A1299 0.23 -0.5 1,532,619 + Rru_A1299 0.23 -1.2 1,532,620 - Rru_A1299 0.23 -2.2 1,533,092 - Rru_A1299 0.57 -0.2 1,533,277 + Rru_A1299 0.71 -2.6 1,533,278 - Rru_A1299 0.71 -1.1 1,533,676 + -0.2 1,533,685 + -3.0 1,533,686 - -1.1 1,533,686 - -2.7 1,533,686 - -0.2 1,533,766 + Rru_A1300 0.10 -1.8 1,533,766 + Rru_A1300 0.10 -0.2 1,533,767 - Rru_A1300 0.10 -1.0 1,533,782 + Rru_A1300 0.12 -2.4 1,533,783 - Rru_A1300 0.12 -0.2 1,533,783 - Rru_A1300 0.12 -0.2 1,533,784 + Rru_A1300 0.13 -0.2 1,533,784 + Rru_A1300 0.13 -0.2 1,533,785 - Rru_A1300 0.13 -0.2 1,533,951 + Rru_A1300 0.34 -0.2 1,534,043 - Rru_A1300 0.45 -0.2 1,534,172 - Rru_A1300 0.61 -0.2 1,534,466 - -0.2 1,534,466 - -0.2 1,534,466 - -0.2 1,534,466 - -1.2 1,534,592 + -4.1 1,534,592 + -0.2
Or see this region's nucleotide sequence