Experiment: MoLS4 with L-Glutamine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt DORF2111 and escJ are separated by 24 nucleotides escJ and DORF2116 are separated by 6 nucleotides DORF2116 and DVUA0118 overlap by 22 nucleotides DVUA0118 and sctN are separated by 327 nucleotides
DORF2111: DORF2111 - SEED: FIG00602408: hypothetical protein, at 154,130 to 154,945
DORF2111
DVUA0117: escJ - type III secretion lipoprotein (TIGR), at 154,970 to 155,785
escJ
DORF2116: DORF2116 - SEED: FIG00606188: hypothetical protein, at 155,792 to 156,715
DORF2116
DVUA0118: DVUA0118 - type III secretion protein, YopL family (TIGR), at 156,694 to 157,311
DVUA0118
DVUA0119: sctN - type III secretion system ATPase (TIGR), at 157,639 to 158,955
sctN
Position (kb) 155
156
157 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 154.834 kb on + strand, within DORF2111 at 154.841 kb on + strand, within DORF2111 at 154.849 kb on - strand, within DORF2111 at 154.886 kb on + strand at 154.892 kb on - strand at 154.904 kb on + strand at 154.976 kb on - strand at 154.997 kb on + strand at 155.041 kb on - strand at 155.095 kb on + strand, within escJ at 155.103 kb on - strand, within escJ at 155.103 kb on - strand, within escJ at 155.157 kb on - strand, within escJ at 155.166 kb on + strand, within escJ at 155.174 kb on - strand, within escJ at 155.190 kb on + strand, within escJ at 155.268 kb on + strand, within escJ at 155.445 kb on + strand, within escJ at 155.459 kb on - strand, within escJ at 155.512 kb on + strand, within escJ at 155.687 kb on + strand, within escJ at 155.765 kb on + strand at 155.775 kb on + strand at 155.780 kb on + strand at 155.839 kb on + strand at 155.839 kb on + strand at 155.847 kb on - strand at 156.146 kb on + strand, within DORF2116 at 156.160 kb on + strand, within DORF2116 at 156.206 kb on - strand, within DORF2116 at 156.231 kb on + strand, within DORF2116 at 156.231 kb on + strand, within DORF2116 at 156.235 kb on + strand, within DORF2116 at 156.236 kb on + strand, within DORF2116 at 156.254 kb on + strand, within DORF2116 at 156.325 kb on - strand, within DORF2116 at 156.339 kb on - strand, within DORF2116 at 156.344 kb on + strand, within DORF2116 at 156.419 kb on + strand, within DORF2116 at 156.419 kb on + strand, within DORF2116 at 156.574 kb on + strand, within DORF2116 at 156.608 kb on - strand, within DORF2116 at 156.713 kb on - strand at 156.887 kb on + strand, within DVUA0118 at 156.895 kb on - strand, within DVUA0118 at 156.922 kb on + strand, within DVUA0118 at 156.934 kb on + strand, within DVUA0118 at 156.949 kb on - strand, within DVUA0118 at 157.445 kb on - strand at 157.459 kb on + strand at 157.474 kb on + strand at 157.482 kb on - strand at 157.482 kb on - strand at 157.562 kb on - strand at 157.607 kb on + strand at 157.665 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction MoLS4 with L-Glutamine (N) remove 154,834 + DORF2111 0.86 +1.5 154,841 + DORF2111 0.87 -0.9 154,849 - DORF2111 0.88 +0.3 154,886 + +0.2 154,892 - -0.0 154,904 + -0.1 154,976 - +0.3 154,997 + +1.3 155,041 - -0.6 155,095 + escJ DVUA0117 0.15 -0.1 155,103 - escJ DVUA0117 0.16 -0.5 155,103 - escJ DVUA0117 0.16 +0.2 155,157 - escJ DVUA0117 0.23 -0.6 155,166 + escJ DVUA0117 0.24 -0.3 155,174 - escJ DVUA0117 0.25 -0.2 155,190 + escJ DVUA0117 0.27 -0.1 155,268 + escJ DVUA0117 0.37 -0.4 155,445 + escJ DVUA0117 0.58 +0.9 155,459 - escJ DVUA0117 0.60 -0.7 155,512 + escJ DVUA0117 0.66 +1.4 155,687 + escJ DVUA0117 0.88 -2.8 155,765 + +0.4 155,775 + +0.3 155,780 + -3.2 155,839 + -0.4 155,839 + -0.4 155,847 - +0.1 156,146 + DORF2116 0.38 -0.1 156,160 + DORF2116 0.40 -3.0 156,206 - DORF2116 0.45 -0.3 156,231 + DORF2116 0.48 -1.5 156,231 + DORF2116 0.48 -0.3 156,235 + DORF2116 0.48 -1.6 156,236 + DORF2116 0.48 -0.3 156,254 + DORF2116 0.50 -2.7 156,325 - DORF2116 0.58 -0.3 156,339 - DORF2116 0.59 -2.5 156,344 + DORF2116 0.60 -1.1 156,419 + DORF2116 0.68 -0.6 156,419 + DORF2116 0.68 -0.0 156,574 + DORF2116 0.85 +0.8 156,608 - DORF2116 0.88 +0.2 156,713 - -1.1 156,887 + DVUA0118 0.31 +0.1 156,895 - DVUA0118 0.33 +0.0 156,922 + DVUA0118 0.37 +0.1 156,934 + DVUA0118 0.39 -1.2 156,949 - DVUA0118 0.41 -0.4 157,445 - -0.2 157,459 + -3.3 157,474 + +0.3 157,482 - -0.1 157,482 - -2.3 157,562 - -0.3 157,607 + -0.0 157,665 - -0.7
Or see this region's nucleotide sequence