Experiment: MoLS4 with L-Glutamine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt DVUA0114 and DVUA0115 are separated by 25 nucleotides DVUA0115 and DVUA0116 are separated by 194 nucleotides DVUA0116 and DORF2111 are separated by 42 nucleotides DORF2111 and escJ are separated by 24 nucleotides escJ and DORF2116 are separated by 6 nucleotides
DVUA0114: DVUA0114 - hypothetical protein (TIGR), at 153,041 to 153,250
DVUA0114
DVUA0115: DVUA0115 - type III secretion system protein, YscF family (TIGR), at 153,276 to 153,527
DVUA0115
DVUA0116: DVUA0116 - conserved hypothetical protein (TIGR), at 153,722 to 154,087
DVUA0116
DORF2111: DORF2111 - SEED: FIG00602408: hypothetical protein, at 154,130 to 154,945
DORF2111
DVUA0117: escJ - type III secretion lipoprotein (TIGR), at 154,970 to 155,785
escJ
DORF2116: DORF2116 - SEED: FIG00606188: hypothetical protein, at 155,792 to 156,715
DORF2116
Position (kb) 154
155 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2 at 153.134 kb on - strand, within DVUA0114 at 153.134 kb on - strand, within DVUA0114 at 153.134 kb on - strand, within DVUA0114 at 153.238 kb on - strand at 153.238 kb on - strand at 153.298 kb on + strand at 153.325 kb on - strand, within DVUA0115 at 153.327 kb on + strand, within DVUA0115 at 153.350 kb on + strand, within DVUA0115 at 153.350 kb on + strand, within DVUA0115 at 153.368 kb on - strand, within DVUA0115 at 153.393 kb on + strand, within DVUA0115 at 153.398 kb on + strand, within DVUA0115 at 153.411 kb on - strand, within DVUA0115 at 153.411 kb on - strand, within DVUA0115 at 153.411 kb on - strand, within DVUA0115 at 153.446 kb on - strand, within DVUA0115 at 153.446 kb on - strand, within DVUA0115 at 153.451 kb on - strand, within DVUA0115 at 153.451 kb on - strand, within DVUA0115 at 153.482 kb on + strand, within DVUA0115 at 153.522 kb on + strand at 153.522 kb on + strand at 153.530 kb on - strand at 153.530 kb on - strand at 153.539 kb on + strand at 153.557 kb on + strand at 153.670 kb on + strand at 153.670 kb on + strand at 153.699 kb on - strand at 153.712 kb on + strand at 153.720 kb on - strand at 153.808 kb on + strand, within DVUA0116 at 153.816 kb on - strand, within DVUA0116 at 153.871 kb on - strand, within DVUA0116 at 153.872 kb on - strand, within DVUA0116 at 153.967 kb on - strand, within DVUA0116 at 153.973 kb on + strand, within DVUA0116 at 153.974 kb on + strand, within DVUA0116 at 154.003 kb on + strand, within DVUA0116 at 154.027 kb on + strand, within DVUA0116 at 154.085 kb on - strand at 154.167 kb on + strand at 154.210 kb on + strand at 154.245 kb on + strand, within DORF2111 at 154.282 kb on + strand, within DORF2111 at 154.344 kb on + strand, within DORF2111 at 154.362 kb on - strand, within DORF2111 at 154.445 kb on + strand, within DORF2111 at 154.452 kb on + strand, within DORF2111 at 154.452 kb on + strand, within DORF2111 at 154.465 kb on + strand, within DORF2111 at 154.500 kb on + strand, within DORF2111 at 154.534 kb on + strand, within DORF2111 at 154.542 kb on - strand, within DORF2111 at 154.542 kb on - strand, within DORF2111 at 154.589 kb on + strand, within DORF2111 at 154.656 kb on - strand, within DORF2111 at 154.665 kb on + strand, within DORF2111 at 154.699 kb on - strand, within DORF2111 at 154.722 kb on - strand, within DORF2111 at 154.834 kb on + strand, within DORF2111 at 154.841 kb on + strand, within DORF2111 at 154.849 kb on - strand, within DORF2111 at 154.886 kb on + strand at 154.892 kb on - strand at 154.904 kb on + strand at 154.976 kb on - strand at 154.997 kb on + strand at 155.041 kb on - strand at 155.095 kb on + strand, within escJ at 155.103 kb on - strand, within escJ at 155.103 kb on - strand, within escJ at 155.157 kb on - strand, within escJ at 155.166 kb on + strand, within escJ at 155.174 kb on - strand, within escJ at 155.190 kb on + strand, within escJ at 155.268 kb on + strand, within escJ at 155.445 kb on + strand, within escJ at 155.459 kb on - strand, within escJ at 155.512 kb on + strand, within escJ at 155.687 kb on + strand, within escJ at 155.765 kb on + strand at 155.775 kb on + strand at 155.780 kb on + strand at 155.839 kb on + strand at 155.839 kb on + strand at 155.847 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction MoLS4 with L-Glutamine (N) remove 153,134 - DVUA0114 0.44 -0.3 153,134 - DVUA0114 0.44 -0.3 153,134 - DVUA0114 0.44 +1.9 153,238 - -0.3 153,238 - -0.0 153,298 + -2.7 153,325 - DVUA0115 0.19 +0.2 153,327 + DVUA0115 0.20 +0.0 153,350 + DVUA0115 0.29 -2.5 153,350 + DVUA0115 0.29 +0.4 153,368 - DVUA0115 0.37 -0.1 153,393 + DVUA0115 0.46 -3.7 153,398 + DVUA0115 0.48 +0.0 153,411 - DVUA0115 0.54 -0.1 153,411 - DVUA0115 0.54 -0.1 153,411 - DVUA0115 0.54 +0.3 153,446 - DVUA0115 0.67 -1.9 153,446 - DVUA0115 0.67 +0.0 153,451 - DVUA0115 0.69 -0.0 153,451 - DVUA0115 0.69 -1.8 153,482 + DVUA0115 0.82 -0.2 153,522 + -2.6 153,522 + +0.4 153,530 - -3.6 153,530 - -0.9 153,539 + -0.3 153,557 + -0.3 153,670 + -0.3 153,670 + -0.1 153,699 - -0.4 153,712 + -0.2 153,720 - +0.3 153,808 + DVUA0116 0.23 +0.2 153,816 - DVUA0116 0.26 -3.4 153,871 - DVUA0116 0.41 -0.4 153,872 - DVUA0116 0.41 -0.4 153,967 - DVUA0116 0.67 -0.5 153,973 + DVUA0116 0.69 +0.2 153,974 + DVUA0116 0.69 +1.5 154,003 + DVUA0116 0.77 -0.5 154,027 + DVUA0116 0.83 -0.9 154,085 - +0.7 154,167 + -0.6 154,210 + -3.2 154,245 + DORF2111 0.14 -0.5 154,282 + DORF2111 0.19 -4.1 154,344 + DORF2111 0.26 -0.4 154,362 - DORF2111 0.28 -3.9 154,445 + DORF2111 0.39 +0.0 154,452 + DORF2111 0.39 -3.5 154,452 + DORF2111 0.39 +0.3 154,465 + DORF2111 0.41 +0.1 154,500 + DORF2111 0.45 -2.9 154,534 + DORF2111 0.50 -0.5 154,542 - DORF2111 0.50 -2.3 154,542 - DORF2111 0.50 -3.8 154,589 + DORF2111 0.56 -0.4 154,656 - DORF2111 0.64 +0.0 154,665 + DORF2111 0.66 +0.2 154,699 - DORF2111 0.70 +0.2 154,722 - DORF2111 0.73 -0.3 154,834 + DORF2111 0.86 +1.5 154,841 + DORF2111 0.87 -0.9 154,849 - DORF2111 0.88 +0.3 154,886 + +0.2 154,892 - -0.0 154,904 + -0.1 154,976 - +0.3 154,997 + +1.3 155,041 - -0.6 155,095 + escJ DVUA0117 0.15 -0.1 155,103 - escJ DVUA0117 0.16 -0.5 155,103 - escJ DVUA0117 0.16 +0.2 155,157 - escJ DVUA0117 0.23 -0.6 155,166 + escJ DVUA0117 0.24 -0.3 155,174 - escJ DVUA0117 0.25 -0.2 155,190 + escJ DVUA0117 0.27 -0.1 155,268 + escJ DVUA0117 0.37 -0.4 155,445 + escJ DVUA0117 0.58 +0.9 155,459 - escJ DVUA0117 0.60 -0.7 155,512 + escJ DVUA0117 0.66 +1.4 155,687 + escJ DVUA0117 0.88 -2.8 155,765 + +0.4 155,775 + +0.3 155,780 + -3.2 155,839 + -0.4 155,839 + -0.4 155,847 - +0.1
Or see this region's nucleotide sequence