Experiment: KB with Nitrofurantoin 0.25 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_3204 and Psyr_3205 are separated by 10 nucleotides Psyr_3205 and Psyr_3206 overlap by 4 nucleotides Psyr_3206 and Psyr_3207 overlap by 4 nucleotides Psyr_3207 and Psyr_3208 are separated by 39 nucleotides
Psyr_3204: Psyr_3204 - NADH dehydrogenase subunit I, at 3,836,385 to 3,836,933
_3204
Psyr_3205: Psyr_3205 - NADH dehydrogenase subunit J, at 3,836,944 to 3,837,453
_3205
Psyr_3206: Psyr_3206 - NADH dehydrogenase subunit K, at 3,837,450 to 3,837,758
_3206
Psyr_3207: Psyr_3207 - NADH dehydrogenase subunit L, at 3,837,755 to 3,839,608
_3207
Psyr_3208: Psyr_3208 - NADH dehydrogenase subunit M, at 3,839,648 to 3,841,180
_3208
Position (kb) 3837
3838
3839
3840 Strain fitness (log2 ratio) -2
-1
0
1 at 3836.785 kb on - strand, within Psyr_3204 at 3837.268 kb on + strand, within Psyr_3205 at 3837.915 kb on + strand at 3837.916 kb on - strand at 3837.998 kb on + strand, within Psyr_3207 at 3838.076 kb on + strand, within Psyr_3207 at 3838.198 kb on - strand, within Psyr_3207 at 3839.043 kb on - strand, within Psyr_3207 at 3839.439 kb on + strand at 3840.315 kb on - strand, within Psyr_3208
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Nitrofurantoin 0.25 mg/ml remove 3,836,785 - Psyr_3204 0.73 +0.2 3,837,268 + Psyr_3205 0.64 +1.2 3,837,915 + -1.3 3,837,916 - -0.8 3,837,998 + Psyr_3207 0.13 -1.6 3,838,076 + Psyr_3207 0.17 +0.8 3,838,198 - Psyr_3207 0.24 -1.4 3,839,043 - Psyr_3207 0.69 -0.8 3,839,439 + +0.2 3,840,315 - Psyr_3208 0.44 -2.1
Or see this region's nucleotide sequence