Experiment: KB with Nitrofurantoin 0.25 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_1647 and Psyr_1648 are separated by 193 nucleotides Psyr_1648 and Psyr_1649 are separated by 114 nucleotides Psyr_1649 and Psyr_1650 overlap by 1 nucleotides Psyr_1650 and Psyr_1651 are separated by 20 nucleotides
Psyr_1647: Psyr_1647 - 3-oxoacyl-[acyl-carrier-protein] reductase, at 1,852,511 to 1,853,254
_1647
Psyr_1648: Psyr_1648 - Acyl carrier protein (ACP), at 1,853,448 to 1,853,690
_1648
Psyr_1649: Psyr_1649 - 3-oxoacyl-[acyl-carrier-protein] synthase II, at 1,853,805 to 1,855,049
_1649
Psyr_1650: Psyr_1650 - aminodeoxychorismate lyase apoprotein, at 1,855,049 to 1,855,864
_1650
Psyr_1651: Psyr_1651 - Protein of unknown function DUF175, at 1,855,885 to 1,857,000
_1651
Position (kb) 1853
1854
1855
1856 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2 at 1853.243 kb on + strand at 1853.252 kb on + strand at 1853.252 kb on + strand at 1853.252 kb on + strand at 1853.252 kb on + strand at 1853.252 kb on + strand at 1853.283 kb on + strand at 1853.306 kb on + strand at 1853.306 kb on + strand at 1853.319 kb on + strand at 1853.319 kb on + strand at 1853.390 kb on + strand at 1853.392 kb on + strand at 1853.392 kb on + strand at 1854.228 kb on - strand, within Psyr_1649 at 1855.047 kb on + strand at 1855.491 kb on + strand, within Psyr_1650 at 1855.563 kb on + strand, within Psyr_1650 at 1855.563 kb on + strand, within Psyr_1650 at 1855.574 kb on + strand, within Psyr_1650 at 1855.749 kb on + strand, within Psyr_1650 at 1855.763 kb on + strand, within Psyr_1650 at 1855.880 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Nitrofurantoin 0.25 mg/ml remove 1,853,243 + +1.8 1,853,252 + +0.9 1,853,252 + +0.8 1,853,252 + +0.6 1,853,252 + -1.1 1,853,252 + +0.4 1,853,283 + +0.4 1,853,306 + +1.3 1,853,306 + +0.9 1,853,319 + +0.1 1,853,319 + +0.2 1,853,390 + +2.0 1,853,392 + +0.9 1,853,392 + +0.1 1,854,228 - Psyr_1649 0.34 +0.7 1,855,047 + -2.9 1,855,491 + Psyr_1650 0.54 -1.3 1,855,563 + Psyr_1650 0.63 -1.9 1,855,563 + Psyr_1650 0.63 -4.5 1,855,574 + Psyr_1650 0.64 -2.6 1,855,749 + Psyr_1650 0.86 -0.9 1,855,763 + Psyr_1650 0.88 -1.2 1,855,880 + +1.9
Or see this region's nucleotide sequence