Experiment: KB with Nitrofurantoin 0.25 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_1626 and Psyr_1627 are separated by 190 nucleotides Psyr_1627 and Psyr_1628 are separated by 76 nucleotides Psyr_1628 and Psyr_1629 are separated by 160 nucleotides Psyr_1629 and Psyr_1630 are separated by 183 nucleotides
Psyr_1626: Psyr_1626 - Pirin, N-terminal:Pirin, C-terminal, at 1,828,914 to 1,829,768
_1626
Psyr_1627: Psyr_1627 - conserved hypothetical protein, at 1,829,959 to 1,830,369
_1627
Psyr_1628: Psyr_1628 - Acyl-CoA dehydrogenase, C-terminal:Acyl-CoA dehydrogenase, N-terminal, at 1,830,446 to 1,832,893
_1628
Psyr_1629: Psyr_1629 - Glutathione S-transferase, N-terminal:Glutathione S-transferase, C-terminal, at 1,833,054 to 1,833,677
_1629
Psyr_1630: Psyr_1630 - ABC transporter, at 1,833,861 to 1,834,793
_1630
Position (kb) 1830
1831
1832
1833 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 1829.560 kb on + strand, within Psyr_1626 at 1829.560 kb on + strand, within Psyr_1626 at 1829.560 kb on + strand, within Psyr_1626 at 1829.560 kb on + strand, within Psyr_1626 at 1829.560 kb on + strand, within Psyr_1626 at 1829.560 kb on + strand, within Psyr_1626 at 1829.561 kb on - strand, within Psyr_1626 at 1829.561 kb on - strand, within Psyr_1626 at 1829.561 kb on - strand, within Psyr_1626 at 1829.561 kb on - strand, within Psyr_1626 at 1829.561 kb on - strand, within Psyr_1626 at 1829.561 kb on - strand, within Psyr_1626 at 1829.561 kb on - strand, within Psyr_1626 at 1829.611 kb on + strand, within Psyr_1626 at 1829.611 kb on + strand, within Psyr_1626 at 1829.611 kb on + strand, within Psyr_1626 at 1829.611 kb on + strand, within Psyr_1626 at 1829.611 kb on + strand, within Psyr_1626 at 1829.611 kb on + strand, within Psyr_1626 at 1829.612 kb on - strand, within Psyr_1626 at 1829.612 kb on - strand, within Psyr_1626 at 1829.612 kb on - strand, within Psyr_1626 at 1829.753 kb on + strand at 1829.797 kb on + strand at 1829.812 kb on + strand at 1829.813 kb on - strand at 1829.813 kb on - strand at 1829.823 kb on - strand at 1830.069 kb on - strand, within Psyr_1627 at 1830.069 kb on - strand, within Psyr_1627 at 1830.405 kb on + strand at 1830.405 kb on + strand at 1830.405 kb on + strand at 1830.407 kb on + strand at 1830.407 kb on + strand at 1830.407 kb on + strand at 1830.408 kb on - strand at 1830.783 kb on - strand, within Psyr_1628 at 1830.849 kb on - strand, within Psyr_1628 at 1830.849 kb on - strand, within Psyr_1628 at 1831.017 kb on + strand, within Psyr_1628 at 1831.017 kb on + strand, within Psyr_1628 at 1831.017 kb on + strand, within Psyr_1628 at 1831.018 kb on - strand, within Psyr_1628 at 1831.018 kb on - strand, within Psyr_1628 at 1831.018 kb on - strand, within Psyr_1628 at 1831.018 kb on - strand, within Psyr_1628 at 1831.018 kb on - strand, within Psyr_1628 at 1831.018 kb on - strand, within Psyr_1628 at 1831.018 kb on - strand, within Psyr_1628 at 1831.018 kb on - strand, within Psyr_1628 at 1831.149 kb on + strand, within Psyr_1628 at 1831.150 kb on - strand, within Psyr_1628 at 1831.326 kb on - strand, within Psyr_1628 at 1831.441 kb on - strand, within Psyr_1628 at 1831.499 kb on - strand, within Psyr_1628 at 1831.528 kb on - strand, within Psyr_1628 at 1831.698 kb on + strand, within Psyr_1628 at 1831.699 kb on - strand, within Psyr_1628 at 1831.722 kb on + strand, within Psyr_1628 at 1831.723 kb on - strand, within Psyr_1628 at 1831.723 kb on - strand, within Psyr_1628 at 1831.834 kb on - strand, within Psyr_1628 at 1832.049 kb on + strand, within Psyr_1628 at 1832.049 kb on + strand, within Psyr_1628 at 1832.049 kb on + strand, within Psyr_1628 at 1832.049 kb on + strand, within Psyr_1628 at 1832.049 kb on + strand, within Psyr_1628 at 1832.049 kb on + strand, within Psyr_1628 at 1832.050 kb on - strand, within Psyr_1628 at 1832.211 kb on + strand, within Psyr_1628 at 1832.211 kb on + strand, within Psyr_1628 at 1832.212 kb on - strand, within Psyr_1628 at 1832.212 kb on - strand, within Psyr_1628 at 1832.212 kb on - strand, within Psyr_1628 at 1832.212 kb on - strand, within Psyr_1628 at 1832.212 kb on - strand, within Psyr_1628 at 1832.239 kb on - strand, within Psyr_1628 at 1832.779 kb on + strand at 1832.780 kb on - strand at 1832.780 kb on - strand at 1832.796 kb on + strand at 1832.796 kb on + strand at 1832.804 kb on + strand at 1832.804 kb on + strand at 1832.893 kb on + strand at 1832.893 kb on + strand at 1832.893 kb on + strand at 1832.893 kb on + strand at 1832.893 kb on + strand at 1832.893 kb on + strand at 1832.893 kb on + strand at 1832.893 kb on + strand at 1832.893 kb on + strand at 1832.893 kb on + strand at 1832.894 kb on - strand at 1832.894 kb on - strand at 1832.894 kb on - strand at 1832.894 kb on - strand at 1832.894 kb on - strand at 1832.894 kb on - strand at 1832.894 kb on - strand at 1832.897 kb on + strand at 1832.897 kb on + strand at 1832.897 kb on + strand at 1832.897 kb on + strand at 1832.897 kb on + strand at 1832.897 kb on + strand at 1832.897 kb on + strand at 1832.897 kb on + strand at 1832.897 kb on + strand at 1832.898 kb on - strand at 1832.898 kb on - strand at 1832.898 kb on - strand at 1832.898 kb on - strand at 1832.898 kb on - strand at 1833.010 kb on + strand at 1833.010 kb on + strand at 1833.010 kb on + strand at 1833.025 kb on + strand at 1833.025 kb on + strand at 1833.025 kb on + strand at 1833.025 kb on + strand at 1833.025 kb on + strand at 1833.026 kb on - strand at 1833.026 kb on - strand at 1833.029 kb on + strand at 1833.029 kb on + strand at 1833.030 kb on - strand at 1833.183 kb on + strand, within Psyr_1629 at 1833.301 kb on + strand, within Psyr_1629 at 1833.301 kb on + strand, within Psyr_1629 at 1833.301 kb on + strand, within Psyr_1629 at 1833.301 kb on + strand, within Psyr_1629 at 1833.302 kb on - strand, within Psyr_1629 at 1833.302 kb on - strand, within Psyr_1629 at 1833.303 kb on + strand, within Psyr_1629 at 1833.304 kb on - strand, within Psyr_1629 at 1833.618 kb on + strand at 1833.619 kb on - strand at 1833.731 kb on + strand at 1833.735 kb on + strand at 1833.735 kb on + strand at 1833.736 kb on - strand at 1833.738 kb on + strand at 1833.738 kb on + strand at 1833.739 kb on - strand at 1833.739 kb on - strand at 1833.741 kb on + strand at 1833.742 kb on - strand at 1833.756 kb on + strand at 1833.756 kb on + strand at 1833.757 kb on - strand at 1833.848 kb on + strand at 1833.848 kb on + strand at 1833.861 kb on - strand at 1833.889 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Nitrofurantoin 0.25 mg/ml remove 1,829,560 + Psyr_1626 0.76 +0.6 1,829,560 + Psyr_1626 0.76 -1.1 1,829,560 + Psyr_1626 0.76 +1.8 1,829,560 + Psyr_1626 0.76 -0.7 1,829,560 + Psyr_1626 0.76 +0.5 1,829,560 + Psyr_1626 0.76 -0.6 1,829,561 - Psyr_1626 0.76 +1.0 1,829,561 - Psyr_1626 0.76 +0.7 1,829,561 - Psyr_1626 0.76 -1.1 1,829,561 - Psyr_1626 0.76 -0.1 1,829,561 - Psyr_1626 0.76 -0.8 1,829,561 - Psyr_1626 0.76 +0.2 1,829,561 - Psyr_1626 0.76 +0.2 1,829,611 + Psyr_1626 0.82 +0.2 1,829,611 + Psyr_1626 0.82 -0.0 1,829,611 + Psyr_1626 0.82 -0.4 1,829,611 + Psyr_1626 0.82 -0.3 1,829,611 + Psyr_1626 0.82 -0.1 1,829,611 + Psyr_1626 0.82 -0.1 1,829,612 - Psyr_1626 0.82 +1.0 1,829,612 - Psyr_1626 0.82 +1.1 1,829,612 - Psyr_1626 0.82 +0.0 1,829,753 + -0.4 1,829,797 + -1.0 1,829,812 + +0.5 1,829,813 - +0.5 1,829,813 - +2.0 1,829,823 - +0.1 1,830,069 - Psyr_1627 0.27 +0.4 1,830,069 - Psyr_1627 0.27 +1.0 1,830,405 + +1.3 1,830,405 + -0.1 1,830,405 + +0.9 1,830,407 + +2.8 1,830,407 + +0.2 1,830,407 + -0.5 1,830,408 - +1.0 1,830,783 - Psyr_1628 0.14 +0.6 1,830,849 - Psyr_1628 0.16 +1.6 1,830,849 - Psyr_1628 0.16 +0.7 1,831,017 + Psyr_1628 0.23 +0.4 1,831,017 + Psyr_1628 0.23 +0.1 1,831,017 + Psyr_1628 0.23 +1.7 1,831,018 - Psyr_1628 0.23 +0.8 1,831,018 - Psyr_1628 0.23 +0.0 1,831,018 - Psyr_1628 0.23 -1.9 1,831,018 - Psyr_1628 0.23 -0.6 1,831,018 - Psyr_1628 0.23 +0.5 1,831,018 - Psyr_1628 0.23 -0.6 1,831,018 - Psyr_1628 0.23 -2.1 1,831,018 - Psyr_1628 0.23 -0.0 1,831,149 + Psyr_1628 0.29 -0.0 1,831,150 - Psyr_1628 0.29 +0.3 1,831,326 - Psyr_1628 0.36 +0.0 1,831,441 - Psyr_1628 0.41 -0.5 1,831,499 - Psyr_1628 0.43 -0.2 1,831,528 - Psyr_1628 0.44 -0.2 1,831,698 + Psyr_1628 0.51 +0.3 1,831,699 - Psyr_1628 0.51 +0.3 1,831,722 + Psyr_1628 0.52 +0.2 1,831,723 - Psyr_1628 0.52 -0.2 1,831,723 - Psyr_1628 0.52 +0.0 1,831,834 - Psyr_1628 0.57 +1.5 1,832,049 + Psyr_1628 0.65 -0.1 1,832,049 + Psyr_1628 0.65 -0.4 1,832,049 + Psyr_1628 0.65 -2.7 1,832,049 + Psyr_1628 0.65 +1.5 1,832,049 + Psyr_1628 0.65 +0.3 1,832,049 + Psyr_1628 0.65 -0.2 1,832,050 - Psyr_1628 0.66 -0.1 1,832,211 + Psyr_1628 0.72 +1.6 1,832,211 + Psyr_1628 0.72 +0.6 1,832,212 - Psyr_1628 0.72 -0.7 1,832,212 - Psyr_1628 0.72 +1.2 1,832,212 - Psyr_1628 0.72 -0.6 1,832,212 - Psyr_1628 0.72 -0.6 1,832,212 - Psyr_1628 0.72 -0.1 1,832,239 - Psyr_1628 0.73 -0.7 1,832,779 + +0.8 1,832,780 - +0.0 1,832,780 - -0.8 1,832,796 + +0.1 1,832,796 + -0.4 1,832,804 + -0.2 1,832,804 + -0.4 1,832,893 + -0.8 1,832,893 + +2.4 1,832,893 + -1.2 1,832,893 + +1.4 1,832,893 + +0.6 1,832,893 + +1.4 1,832,893 + +0.1 1,832,893 + -0.3 1,832,893 + +0.5 1,832,893 + +1.0 1,832,894 - -2.1 1,832,894 - +1.0 1,832,894 - +0.4 1,832,894 - +0.9 1,832,894 - -0.8 1,832,894 - +1.7 1,832,894 - -1.3 1,832,897 + +0.4 1,832,897 + +0.3 1,832,897 + -1.9 1,832,897 + +0.1 1,832,897 + -0.3 1,832,897 + -1.2 1,832,897 + -0.2 1,832,897 + +0.3 1,832,897 + +1.0 1,832,898 - +0.1 1,832,898 - -0.7 1,832,898 - +0.4 1,832,898 - -0.9 1,832,898 - -0.1 1,833,010 + +2.0 1,833,010 + +1.2 1,833,010 + -0.7 1,833,025 + +0.9 1,833,025 + -1.0 1,833,025 + +0.8 1,833,025 + +0.9 1,833,025 + -1.4 1,833,026 - -0.3 1,833,026 - +0.9 1,833,029 + +0.6 1,833,029 + -0.7 1,833,030 - +0.3 1,833,183 + Psyr_1629 0.21 +0.7 1,833,301 + Psyr_1629 0.40 +1.4 1,833,301 + Psyr_1629 0.40 +1.0 1,833,301 + Psyr_1629 0.40 +0.0 1,833,301 + Psyr_1629 0.40 +0.4 1,833,302 - Psyr_1629 0.40 -0.0 1,833,302 - Psyr_1629 0.40 +0.7 1,833,303 + Psyr_1629 0.40 -0.2 1,833,304 - Psyr_1629 0.40 +0.3 1,833,618 + -0.0 1,833,619 - +1.0 1,833,731 + -0.5 1,833,735 + -0.0 1,833,735 + -0.3 1,833,736 - -0.4 1,833,738 + +1.0 1,833,738 + -0.7 1,833,739 - +1.1 1,833,739 - -0.2 1,833,741 + -0.0 1,833,742 - -1.0 1,833,756 + -0.1 1,833,756 + +0.2 1,833,757 - -0.9 1,833,848 + +2.4 1,833,848 + -2.9 1,833,861 - -1.9 1,833,889 + +0.9
Or see this region's nucleotide sequence