Experiment: KB with Nitrofurantoin 0.25 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_1300 and Psyr_1301 are separated by 298 nucleotides Psyr_1301 and Psyr_1302 are separated by 57 nucleotides
Psyr_1300: Psyr_1300 - PAS/PAC sensor hybrid histidine kinase, at 1,468,991 to 1,471,078
_1300
Psyr_1301: Psyr_1301 - Protein of unknown function DUF533, at 1,471,377 to 1,472,123
_1301
Psyr_1302: Psyr_1302 - Leucine-rich repeat protein, at 1,472,181 to 1,478,153
_1302
Position (kb) 1471
1472
1473 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 1470.479 kb on + strand, within Psyr_1300 at 1470.479 kb on + strand, within Psyr_1300 at 1470.751 kb on - strand, within Psyr_1300 at 1470.877 kb on - strand at 1470.921 kb on + strand at 1470.921 kb on + strand at 1470.921 kb on + strand at 1470.921 kb on + strand at 1470.922 kb on - strand at 1470.922 kb on - strand at 1470.922 kb on - strand at 1470.922 kb on - strand at 1471.125 kb on + strand at 1471.125 kb on + strand at 1471.126 kb on - strand at 1471.126 kb on - strand at 1471.171 kb on - strand at 1471.171 kb on - strand at 1471.180 kb on + strand at 1471.181 kb on - strand at 1471.181 kb on - strand at 1471.364 kb on + strand at 1471.364 kb on + strand at 1471.364 kb on + strand at 1471.365 kb on - strand at 1471.383 kb on - strand at 1471.518 kb on - strand, within Psyr_1301 at 1471.518 kb on - strand, within Psyr_1301 at 1471.651 kb on - strand, within Psyr_1301 at 1471.683 kb on + strand, within Psyr_1301 at 1471.683 kb on + strand, within Psyr_1301 at 1471.684 kb on - strand, within Psyr_1301 at 1471.692 kb on + strand, within Psyr_1301 at 1471.692 kb on + strand, within Psyr_1301 at 1471.692 kb on + strand, within Psyr_1301 at 1471.693 kb on - strand, within Psyr_1301 at 1471.782 kb on - strand, within Psyr_1301 at 1471.989 kb on + strand, within Psyr_1301 at 1471.989 kb on + strand, within Psyr_1301 at 1471.989 kb on + strand, within Psyr_1301 at 1471.989 kb on + strand, within Psyr_1301 at 1471.989 kb on + strand, within Psyr_1301 at 1471.989 kb on + strand, within Psyr_1301 at 1471.989 kb on + strand, within Psyr_1301 at 1471.989 kb on + strand, within Psyr_1301 at 1471.989 kb on + strand at 1471.989 kb on + strand, within Psyr_1301 at 1471.989 kb on + strand, within Psyr_1301 at 1471.989 kb on + strand, within Psyr_1301 at 1471.989 kb on + strand, within Psyr_1301 at 1471.989 kb on + strand, within Psyr_1301 at 1471.989 kb on + strand, within Psyr_1301 at 1471.989 kb on + strand, within Psyr_1301 at 1471.989 kb on + strand at 1471.990 kb on - strand, within Psyr_1301 at 1471.990 kb on - strand, within Psyr_1301 at 1471.990 kb on - strand, within Psyr_1301 at 1471.990 kb on - strand, within Psyr_1301 at 1471.990 kb on - strand, within Psyr_1301 at 1471.990 kb on - strand, within Psyr_1301 at 1471.990 kb on - strand, within Psyr_1301 at 1471.990 kb on - strand, within Psyr_1301 at 1471.990 kb on - strand, within Psyr_1301 at 1471.990 kb on - strand, within Psyr_1301 at 1471.990 kb on - strand, within Psyr_1301 at 1471.990 kb on - strand, within Psyr_1301 at 1472.040 kb on + strand, within Psyr_1301 at 1472.279 kb on - strand at 1472.347 kb on + strand at 1472.347 kb on + strand at 1472.395 kb on + strand at 1472.396 kb on - strand at 1472.396 kb on - strand at 1472.583 kb on + strand at 1472.669 kb on - strand at 1472.813 kb on + strand, within Psyr_1302 at 1472.813 kb on + strand, within Psyr_1302 at 1473.088 kb on + strand, within Psyr_1302 at 1473.089 kb on - strand, within Psyr_1302 at 1473.097 kb on + strand, within Psyr_1302
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Nitrofurantoin 0.25 mg/ml remove 1,470,479 + Psyr_1300 0.71 -1.2 1,470,479 + Psyr_1300 0.71 -0.4 1,470,751 - Psyr_1300 0.84 +0.3 1,470,877 - +0.3 1,470,921 + +0.4 1,470,921 + -0.2 1,470,921 + +0.4 1,470,921 + -0.4 1,470,922 - -0.0 1,470,922 - +0.1 1,470,922 - -0.4 1,470,922 - -2.0 1,471,125 + +0.0 1,471,125 + +2.9 1,471,126 - +1.6 1,471,126 - +0.4 1,471,171 - +0.5 1,471,171 - -1.4 1,471,180 + +0.4 1,471,181 - -1.4 1,471,181 - +0.4 1,471,364 + +0.7 1,471,364 + +0.1 1,471,364 + +0.2 1,471,365 - -2.4 1,471,383 - +0.3 1,471,518 - Psyr_1301 0.19 +0.3 1,471,518 - Psyr_1301 0.19 -2.4 1,471,651 - Psyr_1301 0.37 -0.8 1,471,683 + Psyr_1301 0.41 +1.0 1,471,683 + Psyr_1301 0.41 +1.2 1,471,684 - Psyr_1301 0.41 +0.4 1,471,692 + Psyr_1301 0.42 -0.4 1,471,692 + Psyr_1301 0.42 +1.4 1,471,692 + Psyr_1301 0.42 +0.6 1,471,693 - Psyr_1301 0.42 +0.6 1,471,782 - Psyr_1301 0.54 +1.9 1,471,989 + Psyr_1301 0.82 -0.4 1,471,989 + Psyr_1301 0.82 +0.6 1,471,989 + Psyr_1301 0.82 -0.1 1,471,989 + Psyr_1301 0.82 -1.4 1,471,989 + Psyr_1301 0.82 -0.0 1,471,989 + Psyr_1301 0.82 +0.1 1,471,989 + Psyr_1301 0.82 +0.9 1,471,989 + Psyr_1301 0.82 +0.1 1,471,989 + -2.0 1,471,989 + Psyr_1301 0.82 +0.0 1,471,989 + Psyr_1301 0.82 +0.7 1,471,989 + Psyr_1301 0.82 -1.3 1,471,989 + Psyr_1301 0.82 -0.6 1,471,989 + Psyr_1301 0.82 +1.1 1,471,989 + Psyr_1301 0.82 -0.0 1,471,989 + Psyr_1301 0.82 -1.6 1,471,989 + +2.6 1,471,990 - Psyr_1301 0.82 -0.5 1,471,990 - Psyr_1301 0.82 +1.7 1,471,990 - Psyr_1301 0.82 -1.4 1,471,990 - Psyr_1301 0.82 +0.4 1,471,990 - Psyr_1301 0.82 -0.2 1,471,990 - Psyr_1301 0.82 +0.1 1,471,990 - Psyr_1301 0.82 +0.2 1,471,990 - Psyr_1301 0.82 +1.3 1,471,990 - Psyr_1301 0.82 -0.3 1,471,990 - Psyr_1301 0.82 -0.3 1,471,990 - Psyr_1301 0.82 +0.6 1,471,990 - Psyr_1301 0.82 +0.4 1,472,040 + Psyr_1301 0.89 +0.8 1,472,279 - +0.8 1,472,347 + +0.2 1,472,347 + -0.9 1,472,395 + +0.6 1,472,396 - +2.1 1,472,396 - -0.3 1,472,583 + -0.4 1,472,669 - +1.0 1,472,813 + Psyr_1302 0.11 -1.3 1,472,813 + Psyr_1302 0.11 +2.1 1,473,088 + Psyr_1302 0.15 +0.3 1,473,089 - Psyr_1302 0.15 -0.0 1,473,097 + Psyr_1302 0.15 -1.1
Or see this region's nucleotide sequence