Experiment: KB with Nitrofurantoin 0.25 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_1231 and Psyr_1232 are separated by 108 nucleotides Psyr_1232 and Psyr_1233 are separated by 66 nucleotides Psyr_1233 and Psyr_1234 are separated by 215 nucleotides Psyr_1234 and Psyr_1235 overlap by 1 nucleotides
Psyr_1231: Psyr_1231 - protein translocase subunit secF, at 1,389,879 to 1,390,790
_1231
Psyr_1232: Psyr_1232 - conserved hypothetical protein, at 1,390,899 to 1,391,438
_1232
Psyr_1233: Psyr_1233 - Inositol monophosphatase, at 1,391,505 to 1,392,320
_1233
Psyr_1234: Psyr_1234 - RNA methyltransferase TrmH, group 1, at 1,392,536 to 1,393,300
_1234
Psyr_1235: Psyr_1235 - serine O-acetyltransferase, at 1,393,300 to 1,394,076
_1235
Position (kb)
1391
1392
1393 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 1390.789 kb on - strand at 1390.803 kb on + strand at 1390.803 kb on + strand at 1390.803 kb on + strand at 1390.804 kb on - strand at 1390.804 kb on - strand at 1390.910 kb on + strand at 1390.910 kb on + strand at 1390.910 kb on + strand at 1390.910 kb on + strand at 1390.910 kb on + strand at 1390.910 kb on + strand at 1390.911 kb on - strand at 1390.911 kb on - strand at 1390.911 kb on - strand at 1390.911 kb on - strand at 1390.930 kb on + strand at 1390.930 kb on + strand at 1390.930 kb on + strand at 1390.931 kb on - strand at 1390.971 kb on + strand, within Psyr_1232 at 1390.971 kb on + strand, within Psyr_1232 at 1390.971 kb on + strand, within Psyr_1232 at 1390.971 kb on + strand, within Psyr_1232 at 1390.971 kb on + strand, within Psyr_1232 at 1390.971 kb on + strand, within Psyr_1232 at 1390.971 kb on + strand, within Psyr_1232 at 1390.971 kb on + strand, within Psyr_1232 at 1390.971 kb on + strand, within Psyr_1232 at 1390.971 kb on + strand, within Psyr_1232 at 1390.971 kb on + strand, within Psyr_1232 at 1390.971 kb on + strand, within Psyr_1232 at 1390.971 kb on + strand, within Psyr_1232 at 1390.972 kb on - strand, within Psyr_1232 at 1390.972 kb on - strand, within Psyr_1232 at 1390.972 kb on - strand, within Psyr_1232 at 1390.972 kb on - strand, within Psyr_1232 at 1390.972 kb on - strand, within Psyr_1232 at 1390.972 kb on - strand, within Psyr_1232 at 1390.972 kb on - strand, within Psyr_1232 at 1390.972 kb on - strand, within Psyr_1232 at 1390.972 kb on - strand, within Psyr_1232 at 1390.972 kb on - strand, within Psyr_1232 at 1390.972 kb on - strand, within Psyr_1232 at 1390.973 kb on + strand, within Psyr_1232 at 1390.973 kb on + strand, within Psyr_1232 at 1390.973 kb on + strand, within Psyr_1232 at 1390.973 kb on + strand, within Psyr_1232 at 1390.973 kb on + strand, within Psyr_1232 at 1390.974 kb on - strand, within Psyr_1232 at 1390.974 kb on - strand, within Psyr_1232 at 1390.974 kb on - strand, within Psyr_1232 at 1390.974 kb on - strand, within Psyr_1232 at 1390.974 kb on - strand, within Psyr_1232 at 1390.974 kb on - strand, within Psyr_1232 at 1390.998 kb on + strand, within Psyr_1232 at 1391.008 kb on + strand, within Psyr_1232 at 1391.008 kb on + strand, within Psyr_1232 at 1391.008 kb on + strand, within Psyr_1232 at 1391.008 kb on + strand, within Psyr_1232 at 1391.008 kb on + strand, within Psyr_1232 at 1391.008 kb on + strand, within Psyr_1232 at 1391.009 kb on - strand, within Psyr_1232 at 1391.069 kb on - strand, within Psyr_1232 at 1391.262 kb on + strand, within Psyr_1232 at 1391.262 kb on + strand, within Psyr_1232 at 1391.263 kb on - strand, within Psyr_1232 at 1391.263 kb on - strand, within Psyr_1232 at 1391.263 kb on - strand, within Psyr_1232 at 1391.263 kb on - strand, within Psyr_1232 at 1391.323 kb on - strand, within Psyr_1232 at 1391.323 kb on - strand, within Psyr_1232 at 1391.334 kb on + strand, within Psyr_1232 at 1391.334 kb on + strand, within Psyr_1232 at 1391.334 kb on + strand, within Psyr_1232 at 1391.335 kb on - strand, within Psyr_1232 at 1391.335 kb on - strand, within Psyr_1232 at 1391.335 kb on - strand, within Psyr_1232 at 1391.335 kb on - strand, within Psyr_1232 at 1391.335 kb on - strand, within Psyr_1232 at 1391.335 kb on - strand, within Psyr_1232 at 1391.335 kb on - strand, within Psyr_1232 at 1391.335 kb on - strand, within Psyr_1232 at 1391.335 kb on - strand, within Psyr_1232 at 1391.335 kb on - strand, within Psyr_1232 at 1391.465 kb on + strand at 1391.466 kb on - strand at 1391.466 kb on - strand at 1391.494 kb on - strand at 1392.473 kb on + strand at 1392.473 kb on + strand at 1392.985 kb on - strand, within Psyr_1234 at 1392.998 kb on + strand, within Psyr_1234 at 1392.998 kb on + strand, within Psyr_1234 at 1392.999 kb on - strand, within Psyr_1234 at 1392.999 kb on - strand, within Psyr_1234 at 1392.999 kb on - strand, within Psyr_1234 at 1392.999 kb on - strand, within Psyr_1234 at 1393.106 kb on + strand, within Psyr_1234 at 1393.107 kb on - strand, within Psyr_1234 at 1393.107 kb on - strand, within Psyr_1234 at 1393.193 kb on + strand, within Psyr_1234 at 1393.194 kb on - strand, within Psyr_1234 at 1393.280 kb on + strand at 1393.280 kb on + strand at 1393.281 kb on - strand at 1393.281 kb on - strand at 1393.281 kb on - strand at 1393.281 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Nitrofurantoin 0.25 mg/ml remove 1,390,789 - -0.3 1,390,803 + +0.1 1,390,803 + +2.2 1,390,803 + +2.9 1,390,804 - +0.1 1,390,804 - +1.3 1,390,910 + +1.0 1,390,910 + -0.9 1,390,910 + +1.6 1,390,910 + +1.2 1,390,910 + +1.1 1,390,910 + -1.8 1,390,911 - +0.5 1,390,911 - +0.7 1,390,911 - +0.1 1,390,911 - +0.5 1,390,930 + +1.5 1,390,930 + +0.2 1,390,930 + +0.0 1,390,931 - +0.0 1,390,971 + Psyr_1232 0.13 -0.4 1,390,971 + Psyr_1232 0.13 +0.7 1,390,971 + Psyr_1232 0.13 +0.6 1,390,971 + Psyr_1232 0.13 +0.8 1,390,971 + Psyr_1232 0.13 +0.1 1,390,971 + Psyr_1232 0.13 +0.9 1,390,971 + Psyr_1232 0.13 -0.8 1,390,971 + Psyr_1232 0.13 -0.9 1,390,971 + Psyr_1232 0.13 +0.8 1,390,971 + Psyr_1232 0.13 +0.9 1,390,971 + Psyr_1232 0.13 +0.8 1,390,971 + Psyr_1232 0.13 +1.1 1,390,971 + Psyr_1232 0.13 -0.8 1,390,972 - Psyr_1232 0.14 -0.3 1,390,972 - Psyr_1232 0.14 +0.9 1,390,972 - Psyr_1232 0.14 +1.2 1,390,972 - Psyr_1232 0.14 -0.2 1,390,972 - Psyr_1232 0.14 +0.4 1,390,972 - Psyr_1232 0.14 +1.4 1,390,972 - Psyr_1232 0.14 -0.1 1,390,972 - Psyr_1232 0.14 -0.5 1,390,972 - Psyr_1232 0.14 +0.6 1,390,972 - Psyr_1232 0.14 -0.2 1,390,972 - Psyr_1232 0.14 +0.7 1,390,973 + Psyr_1232 0.14 +1.6 1,390,973 + Psyr_1232 0.14 +1.0 1,390,973 + Psyr_1232 0.14 +0.4 1,390,973 + Psyr_1232 0.14 +0.6 1,390,973 + Psyr_1232 0.14 -0.1 1,390,974 - Psyr_1232 0.14 +0.5 1,390,974 - Psyr_1232 0.14 -0.7 1,390,974 - Psyr_1232 0.14 +1.2 1,390,974 - Psyr_1232 0.14 +1.4 1,390,974 - Psyr_1232 0.14 -0.0 1,390,974 - Psyr_1232 0.14 -1.4 1,390,998 + Psyr_1232 0.18 +3.6 1,391,008 + Psyr_1232 0.20 -0.2 1,391,008 + Psyr_1232 0.20 +0.1 1,391,008 + Psyr_1232 0.20 +0.6 1,391,008 + Psyr_1232 0.20 +0.2 1,391,008 + Psyr_1232 0.20 +0.5 1,391,008 + Psyr_1232 0.20 -1.0 1,391,009 - Psyr_1232 0.20 +0.9 1,391,069 - Psyr_1232 0.31 +0.8 1,391,262 + Psyr_1232 0.67 +1.1 1,391,262 + Psyr_1232 0.67 -2.9 1,391,263 - Psyr_1232 0.67 -0.3 1,391,263 - Psyr_1232 0.67 -1.0 1,391,263 - Psyr_1232 0.67 +0.7 1,391,263 - Psyr_1232 0.67 +0.6 1,391,323 - Psyr_1232 0.79 +1.2 1,391,323 - Psyr_1232 0.79 +1.1 1,391,334 + Psyr_1232 0.81 -1.1 1,391,334 + Psyr_1232 0.81 +1.6 1,391,334 + Psyr_1232 0.81 -1.3 1,391,335 - Psyr_1232 0.81 +0.9 1,391,335 - Psyr_1232 0.81 +0.6 1,391,335 - Psyr_1232 0.81 -0.3 1,391,335 - Psyr_1232 0.81 +0.0 1,391,335 - Psyr_1232 0.81 +0.3 1,391,335 - Psyr_1232 0.81 -0.8 1,391,335 - Psyr_1232 0.81 +2.2 1,391,335 - Psyr_1232 0.81 +1.2 1,391,335 - Psyr_1232 0.81 -0.9 1,391,335 - Psyr_1232 0.81 -0.4 1,391,465 + +0.3 1,391,466 - -0.8 1,391,466 - -0.8 1,391,494 - +1.1 1,392,473 + +0.4 1,392,473 + +0.6 1,392,985 - Psyr_1234 0.59 -0.4 1,392,998 + Psyr_1234 0.60 +0.5 1,392,998 + Psyr_1234 0.60 -1.0 1,392,999 - Psyr_1234 0.61 -0.1 1,392,999 - Psyr_1234 0.61 -0.6 1,392,999 - Psyr_1234 0.61 -2.4 1,392,999 - Psyr_1234 0.61 -0.8 1,393,106 + Psyr_1234 0.75 +0.1 1,393,107 - Psyr_1234 0.75 -0.2 1,393,107 - Psyr_1234 0.75 -1.4 1,393,193 + Psyr_1234 0.86 +0.8 1,393,194 - Psyr_1234 0.86 +2.2 1,393,280 + +0.9 1,393,280 + -2.3 1,393,281 - -0.6 1,393,281 - +0.1 1,393,281 - +0.1 1,393,281 - -0.8
Or see this region's nucleotide sequence