Experiment: KB with Nitrofurantoin 0.25 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_0609 and Psyr_0610 are separated by 315 nucleotides Psyr_0610 and Psyr_0611 overlap by 4 nucleotides Psyr_0611 and Psyr_0612 are separated by 66 nucleotides
Psyr_0609: Psyr_0609 - Glutathione S-transferase, C-terminal, at 699,376 to 700,218
_0609
Psyr_0610: Psyr_0610 - ABC-2, at 700,534 to 701,310
_0610
Psyr_0611: Psyr_0611 - ABC transporter, at 701,307 to 702,011
_0611
Psyr_0612: Psyr_0612 - Nucleotidyl transferase, at 702,078 to 702,839
_0612
Position (kb)
700
701
702 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 699.545 kb on + strand, within Psyr_0609 at 699.546 kb on - strand, within Psyr_0609 at 699.546 kb on - strand, within Psyr_0609 at 699.572 kb on + strand, within Psyr_0609 at 699.605 kb on + strand, within Psyr_0609 at 699.605 kb on + strand, within Psyr_0609 at 699.605 kb on + strand, within Psyr_0609 at 699.606 kb on - strand, within Psyr_0609 at 699.606 kb on - strand, within Psyr_0609 at 699.606 kb on - strand, within Psyr_0609 at 699.698 kb on + strand, within Psyr_0609 at 699.719 kb on + strand, within Psyr_0609 at 699.720 kb on - strand, within Psyr_0609 at 699.725 kb on + strand, within Psyr_0609 at 699.725 kb on + strand, within Psyr_0609 at 699.725 kb on + strand, within Psyr_0609 at 699.725 kb on + strand, within Psyr_0609 at 699.725 kb on + strand, within Psyr_0609 at 699.725 kb on + strand, within Psyr_0609 at 699.725 kb on + strand, within Psyr_0609 at 699.725 kb on + strand, within Psyr_0609 at 699.726 kb on - strand, within Psyr_0609 at 699.752 kb on + strand, within Psyr_0609 at 699.848 kb on + strand, within Psyr_0609 at 699.848 kb on + strand, within Psyr_0609 at 699.858 kb on + strand, within Psyr_0609 at 699.975 kb on + strand, within Psyr_0609 at 699.975 kb on + strand, within Psyr_0609 at 699.976 kb on - strand, within Psyr_0609 at 699.976 kb on - strand, within Psyr_0609 at 699.995 kb on + strand, within Psyr_0609 at 699.995 kb on + strand, within Psyr_0609 at 700.076 kb on + strand, within Psyr_0609 at 700.076 kb on + strand, within Psyr_0609 at 700.077 kb on - strand, within Psyr_0609 at 700.077 kb on - strand, within Psyr_0609 at 700.077 kb on - strand, within Psyr_0609 at 700.202 kb on + strand at 700.202 kb on + strand at 700.275 kb on + strand at 700.275 kb on + strand at 700.275 kb on + strand at 700.276 kb on - strand at 700.276 kb on - strand at 700.282 kb on + strand at 700.284 kb on + strand at 700.284 kb on + strand at 700.284 kb on + strand at 700.284 kb on + strand at 700.284 kb on + strand at 700.284 kb on + strand at 700.284 kb on + strand at 700.284 kb on + strand at 700.284 kb on + strand at 700.285 kb on - strand at 700.285 kb on - strand at 700.285 kb on - strand at 700.285 kb on - strand at 700.285 kb on - strand at 700.285 kb on - strand at 700.294 kb on + strand at 700.295 kb on - strand at 700.295 kb on - strand at 700.331 kb on + strand at 700.380 kb on + strand at 700.396 kb on - strand at 700.409 kb on - strand at 700.410 kb on + strand at 700.410 kb on + strand at 700.410 kb on + strand at 700.410 kb on + strand at 700.411 kb on - strand at 700.411 kb on - strand at 700.411 kb on - strand at 700.417 kb on - strand at 700.425 kb on - strand at 700.437 kb on + strand at 700.437 kb on + strand at 700.437 kb on + strand at 700.437 kb on + strand at 700.438 kb on - strand at 700.438 kb on - strand at 700.438 kb on - strand at 700.438 kb on - strand at 700.438 kb on - strand at 700.438 kb on - strand at 700.461 kb on + strand at 700.461 kb on + strand at 700.461 kb on + strand at 700.461 kb on + strand at 700.462 kb on - strand at 700.518 kb on - strand at 700.521 kb on - strand at 700.534 kb on - strand at 700.615 kb on + strand, within Psyr_0610 at 700.616 kb on - strand, within Psyr_0610 at 700.616 kb on - strand, within Psyr_0610 at 700.639 kb on - strand, within Psyr_0610 at 700.678 kb on + strand, within Psyr_0610 at 700.678 kb on + strand, within Psyr_0610 at 700.678 kb on + strand, within Psyr_0610 at 700.679 kb on - strand, within Psyr_0610 at 700.679 kb on - strand, within Psyr_0610 at 700.682 kb on - strand, within Psyr_0610 at 700.696 kb on + strand, within Psyr_0610 at 700.696 kb on + strand, within Psyr_0610 at 700.696 kb on + strand, within Psyr_0610 at 700.696 kb on + strand, within Psyr_0610 at 700.696 kb on + strand, within Psyr_0610 at 700.696 kb on + strand, within Psyr_0610 at 700.696 kb on + strand, within Psyr_0610 at 700.697 kb on - strand, within Psyr_0610 at 700.697 kb on - strand, within Psyr_0610 at 700.697 kb on - strand, within Psyr_0610 at 700.697 kb on - strand, within Psyr_0610 at 700.699 kb on - strand, within Psyr_0610 at 700.699 kb on - strand, within Psyr_0610 at 700.710 kb on + strand, within Psyr_0610 at 700.711 kb on - strand, within Psyr_0610 at 700.916 kb on + strand, within Psyr_0610 at 700.916 kb on + strand, within Psyr_0610 at 700.916 kb on + strand, within Psyr_0610 at 700.917 kb on - strand, within Psyr_0610 at 700.917 kb on - strand, within Psyr_0610 at 700.917 kb on - strand, within Psyr_0610 at 700.961 kb on - strand, within Psyr_0610 at 701.070 kb on - strand, within Psyr_0610 at 701.099 kb on + strand, within Psyr_0610 at 701.099 kb on + strand, within Psyr_0610 at 701.099 kb on + strand, within Psyr_0610 at 701.099 kb on + strand, within Psyr_0610 at 701.099 kb on + strand, within Psyr_0610 at 701.099 kb on + strand, within Psyr_0610 at 701.099 kb on + strand, within Psyr_0610 at 701.099 kb on + strand, within Psyr_0610 at 701.100 kb on - strand, within Psyr_0610 at 701.100 kb on - strand, within Psyr_0610 at 701.100 kb on - strand, within Psyr_0610 at 701.100 kb on - strand, within Psyr_0610 at 701.146 kb on + strand, within Psyr_0610 at 701.146 kb on + strand, within Psyr_0610 at 701.146 kb on + strand, within Psyr_0610 at 701.146 kb on + strand, within Psyr_0610 at 701.147 kb on - strand, within Psyr_0610 at 701.147 kb on - strand, within Psyr_0610 at 701.147 kb on - strand, within Psyr_0610 at 701.147 kb on - strand, within Psyr_0610 at 701.164 kb on + strand, within Psyr_0610 at 701.165 kb on - strand, within Psyr_0610 at 701.165 kb on - strand, within Psyr_0610 at 701.179 kb on - strand, within Psyr_0610 at 701.302 kb on + strand at 701.302 kb on + strand at 701.302 kb on + strand at 701.303 kb on - strand at 701.303 kb on - strand at 701.303 kb on - strand at 701.303 kb on - strand at 701.303 kb on - strand at 701.303 kb on - strand at 701.303 kb on - strand at 701.306 kb on + strand at 701.306 kb on + strand at 701.306 kb on + strand at 701.307 kb on - strand at 701.307 kb on - strand at 701.307 kb on - strand at 701.307 kb on - strand at 701.452 kb on + strand, within Psyr_0611 at 701.453 kb on - strand, within Psyr_0611 at 701.453 kb on - strand, within Psyr_0611 at 701.453 kb on - strand, within Psyr_0611 at 701.453 kb on - strand, within Psyr_0611 at 701.453 kb on - strand, within Psyr_0611 at 701.516 kb on + strand, within Psyr_0611 at 701.516 kb on + strand, within Psyr_0611 at 701.517 kb on - strand, within Psyr_0611 at 701.517 kb on - strand, within Psyr_0611 at 701.517 kb on - strand, within Psyr_0611 at 701.517 kb on - strand, within Psyr_0611 at 701.517 kb on - strand, within Psyr_0611 at 701.602 kb on + strand, within Psyr_0611 at 701.642 kb on + strand, within Psyr_0611 at 701.643 kb on - strand, within Psyr_0611 at 701.757 kb on + strand, within Psyr_0611 at 701.773 kb on - strand, within Psyr_0611 at 701.773 kb on - strand, within Psyr_0611 at 701.773 kb on - strand, within Psyr_0611 at 701.773 kb on - strand, within Psyr_0611 at 701.773 kb on - strand, within Psyr_0611 at 701.773 kb on - strand, within Psyr_0611 at 701.965 kb on - strand at 702.071 kb on + strand at 702.072 kb on - strand at 702.072 kb on - strand at 702.163 kb on + strand, within Psyr_0612 at 702.163 kb on + strand, within Psyr_0612 at 702.163 kb on + strand, within Psyr_0612 at 702.163 kb on + strand, within Psyr_0612 at 702.163 kb on + strand, within Psyr_0612 at 702.163 kb on + strand, within Psyr_0612 at 702.164 kb on - strand, within Psyr_0612 at 702.164 kb on - strand, within Psyr_0612 at 702.164 kb on - strand, within Psyr_0612 at 702.164 kb on - strand, within Psyr_0612 at 702.233 kb on + strand, within Psyr_0612 at 702.233 kb on + strand, within Psyr_0612 at 702.233 kb on + strand, within Psyr_0612 at 702.233 kb on + strand, within Psyr_0612 at 702.234 kb on - strand, within Psyr_0612 at 702.234 kb on - strand, within Psyr_0612
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Nitrofurantoin 0.25 mg/ml remove 699,545 + Psyr_0609 0.20 +0.8 699,546 - Psyr_0609 0.20 +1.0 699,546 - Psyr_0609 0.20 +0.3 699,572 + Psyr_0609 0.23 +1.3 699,605 + Psyr_0609 0.27 +0.4 699,605 + Psyr_0609 0.27 -0.5 699,605 + Psyr_0609 0.27 +0.4 699,606 - Psyr_0609 0.27 +0.8 699,606 - Psyr_0609 0.27 -0.1 699,606 - Psyr_0609 0.27 +1.1 699,698 + Psyr_0609 0.38 +0.7 699,719 + Psyr_0609 0.41 +0.1 699,720 - Psyr_0609 0.41 +1.3 699,725 + Psyr_0609 0.41 +0.9 699,725 + Psyr_0609 0.41 +0.6 699,725 + Psyr_0609 0.41 -0.3 699,725 + Psyr_0609 0.41 -0.6 699,725 + Psyr_0609 0.41 +1.6 699,725 + Psyr_0609 0.41 +0.1 699,725 + Psyr_0609 0.41 +0.5 699,725 + Psyr_0609 0.41 +1.2 699,726 - Psyr_0609 0.42 +0.3 699,752 + Psyr_0609 0.45 +0.0 699,848 + Psyr_0609 0.56 +0.0 699,848 + Psyr_0609 0.56 -0.2 699,858 + Psyr_0609 0.57 +0.3 699,975 + Psyr_0609 0.71 -0.7 699,975 + Psyr_0609 0.71 +1.7 699,976 - Psyr_0609 0.71 +0.8 699,976 - Psyr_0609 0.71 +0.7 699,995 + Psyr_0609 0.73 +1.0 699,995 + Psyr_0609 0.73 +0.8 700,076 + Psyr_0609 0.83 +1.0 700,076 + Psyr_0609 0.83 +1.5 700,077 - Psyr_0609 0.83 +0.1 700,077 - Psyr_0609 0.83 -0.2 700,077 - Psyr_0609 0.83 +0.3 700,202 + +1.0 700,202 + -0.9 700,275 + -0.2 700,275 + +0.3 700,275 + +1.1 700,276 - -0.3 700,276 - -0.2 700,282 + -0.2 700,284 + -0.6 700,284 + -0.3 700,284 + +0.0 700,284 + -0.1 700,284 + -0.4 700,284 + -0.4 700,284 + +1.1 700,284 + +0.1 700,284 + +1.6 700,285 - +0.4 700,285 - -0.4 700,285 - +0.1 700,285 - -0.1 700,285 - -0.5 700,285 - -1.8 700,294 + +0.3 700,295 - +1.5 700,295 - -1.3 700,331 + +0.5 700,380 + -0.7 700,396 - +1.7 700,409 - +0.9 700,410 + -2.1 700,410 + +1.0 700,410 + -0.6 700,410 + +0.4 700,411 - -0.1 700,411 - -0.4 700,411 - -1.9 700,417 - -1.2 700,425 - +0.6 700,437 + +0.2 700,437 + +0.6 700,437 + +3.2 700,437 + -0.7 700,438 - -1.7 700,438 - +1.2 700,438 - -1.2 700,438 - +1.4 700,438 - +0.8 700,438 - -0.5 700,461 + +0.2 700,461 + -0.1 700,461 + -0.5 700,461 + +0.5 700,462 - +1.1 700,518 - -2.7 700,521 - +0.9 700,534 - +0.1 700,615 + Psyr_0610 0.10 +0.0 700,616 - Psyr_0610 0.11 +0.6 700,616 - Psyr_0610 0.11 +0.4 700,639 - Psyr_0610 0.14 -0.3 700,678 + Psyr_0610 0.19 +0.1 700,678 + Psyr_0610 0.19 -0.6 700,678 + Psyr_0610 0.19 -0.3 700,679 - Psyr_0610 0.19 -1.2 700,679 - Psyr_0610 0.19 -0.1 700,682 - Psyr_0610 0.19 -0.3 700,696 + Psyr_0610 0.21 +0.8 700,696 + Psyr_0610 0.21 -0.4 700,696 + Psyr_0610 0.21 -0.0 700,696 + Psyr_0610 0.21 -2.2 700,696 + Psyr_0610 0.21 -0.1 700,696 + Psyr_0610 0.21 +0.9 700,696 + Psyr_0610 0.21 -0.8 700,697 - Psyr_0610 0.21 -0.0 700,697 - Psyr_0610 0.21 +0.2 700,697 - Psyr_0610 0.21 +0.0 700,697 - Psyr_0610 0.21 +1.2 700,699 - Psyr_0610 0.21 +0.4 700,699 - Psyr_0610 0.21 +0.0 700,710 + Psyr_0610 0.23 -2.4 700,711 - Psyr_0610 0.23 -0.0 700,916 + Psyr_0610 0.49 +0.9 700,916 + Psyr_0610 0.49 -0.6 700,916 + Psyr_0610 0.49 -1.0 700,917 - Psyr_0610 0.49 -1.3 700,917 - Psyr_0610 0.49 +0.4 700,917 - Psyr_0610 0.49 -0.8 700,961 - Psyr_0610 0.55 -0.9 701,070 - Psyr_0610 0.69 +0.3 701,099 + Psyr_0610 0.73 -0.1 701,099 + Psyr_0610 0.73 +0.2 701,099 + Psyr_0610 0.73 -1.4 701,099 + Psyr_0610 0.73 +0.2 701,099 + Psyr_0610 0.73 +0.9 701,099 + Psyr_0610 0.73 +0.4 701,099 + Psyr_0610 0.73 +2.3 701,099 + Psyr_0610 0.73 -1.0 701,100 - Psyr_0610 0.73 +1.0 701,100 - Psyr_0610 0.73 +0.9 701,100 - Psyr_0610 0.73 +0.1 701,100 - Psyr_0610 0.73 -1.6 701,146 + Psyr_0610 0.79 +0.3 701,146 + Psyr_0610 0.79 -0.2 701,146 + Psyr_0610 0.79 -1.7 701,146 + Psyr_0610 0.79 +0.3 701,147 - Psyr_0610 0.79 +2.8 701,147 - Psyr_0610 0.79 +2.9 701,147 - Psyr_0610 0.79 -2.0 701,147 - Psyr_0610 0.79 +0.5 701,164 + Psyr_0610 0.81 -0.2 701,165 - Psyr_0610 0.81 -1.2 701,165 - Psyr_0610 0.81 -0.3 701,179 - Psyr_0610 0.83 +1.3 701,302 + +0.7 701,302 + -1.8 701,302 + +0.0 701,303 - -0.6 701,303 - -1.0 701,303 - -0.4 701,303 - +0.4 701,303 - -0.6 701,303 - +1.4 701,303 - +0.6 701,306 + +0.9 701,306 + +1.8 701,306 + +2.2 701,307 - +1.9 701,307 - -0.1 701,307 - +1.1 701,307 - -0.4 701,452 + Psyr_0611 0.21 +2.2 701,453 - Psyr_0611 0.21 +0.3 701,453 - Psyr_0611 0.21 +0.6 701,453 - Psyr_0611 0.21 +0.8 701,453 - Psyr_0611 0.21 +0.2 701,453 - Psyr_0611 0.21 -0.3 701,516 + Psyr_0611 0.30 +0.9 701,516 + Psyr_0611 0.30 +0.3 701,517 - Psyr_0611 0.30 -2.0 701,517 - Psyr_0611 0.30 +0.1 701,517 - Psyr_0611 0.30 -0.5 701,517 - Psyr_0611 0.30 -0.4 701,517 - Psyr_0611 0.30 +0.2 701,602 + Psyr_0611 0.42 +0.0 701,642 + Psyr_0611 0.48 +0.7 701,643 - Psyr_0611 0.48 +0.1 701,757 + Psyr_0611 0.64 +0.0 701,773 - Psyr_0611 0.66 -1.7 701,773 - Psyr_0611 0.66 +0.6 701,773 - Psyr_0611 0.66 -0.1 701,773 - Psyr_0611 0.66 +0.4 701,773 - Psyr_0611 0.66 +0.1 701,773 - Psyr_0611 0.66 -0.1 701,965 - +2.6 702,071 + +0.1 702,072 - +2.2 702,072 - +0.1 702,163 + Psyr_0612 0.11 +1.3 702,163 + Psyr_0612 0.11 -0.5 702,163 + Psyr_0612 0.11 +1.1 702,163 + Psyr_0612 0.11 +1.3 702,163 + Psyr_0612 0.11 +0.8 702,163 + Psyr_0612 0.11 -0.3 702,164 - Psyr_0612 0.11 +0.2 702,164 - Psyr_0612 0.11 -1.0 702,164 - Psyr_0612 0.11 +0.6 702,164 - Psyr_0612 0.11 -0.0 702,233 + Psyr_0612 0.20 +0.3 702,233 + Psyr_0612 0.20 +1.2 702,233 + Psyr_0612 0.20 +0.1 702,233 + Psyr_0612 0.20 +1.1 702,234 - Psyr_0612 0.20 -1.9 702,234 - Psyr_0612 0.20 -1.4
Or see this region's nucleotide sequence