Strain Fitness in Pseudomonas syringae pv. syringae B728a around Psyr_0501

Experiment: KB with Nitrofurantoin 0.25 mg/ml

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPsyr_0500 and Psyr_0501 are separated by 58 nucleotidesPsyr_0501 and Psyr_0502 are separated by 23 nucleotides Psyr_0500: Psyr_0500 - biotin carboxyl carrier protein / biotin carboxylase, at 542,495 to 544,228 _0500 Psyr_0501: Psyr_0501 - MscS Mechanosensitive ion channel, at 544,287 to 547,691 _0501 Psyr_0502: Psyr_0502 - potassium/proton antiporter, CPA1 family, at 547,715 to 549,511 _0502 Position (kb) 544 545 546 547 548Strain fitness (log2 ratio) -3 -2 -1 0 1 2 3 4at 543.293 kb on + strand, within Psyr_0500at 543.294 kb on - strand, within Psyr_0500at 543.294 kb on - strand, within Psyr_0500at 543.550 kb on + strand, within Psyr_0500at 543.551 kb on - strand, within Psyr_0500at 543.551 kb on - strand, within Psyr_0500at 543.558 kb on + strand, within Psyr_0500at 543.793 kb on + strand, within Psyr_0500at 543.794 kb on - strand, within Psyr_0500at 544.188 kb on - strandat 544.343 kb on + strandat 544.420 kb on + strandat 544.447 kb on + strandat 544.447 kb on + strandat 544.447 kb on + strandat 544.447 kb on + strandat 544.448 kb on - strandat 544.448 kb on - strandat 544.448 kb on - strandat 544.554 kb on + strandat 544.555 kb on - strandat 544.555 kb on - strandat 544.555 kb on - strandat 544.555 kb on - strandat 544.555 kb on - strandat 544.584 kb on - strandat 544.606 kb on + strandat 544.659 kb on + strand, within Psyr_0501at 544.659 kb on + strand, within Psyr_0501at 544.660 kb on - strand, within Psyr_0501at 545.057 kb on - strand, within Psyr_0501at 545.057 kb on - strand, within Psyr_0501at 545.080 kb on + strand, within Psyr_0501at 545.121 kb on + strand, within Psyr_0501at 545.122 kb on - strand, within Psyr_0501at 545.245 kb on + strand, within Psyr_0501at 545.245 kb on + strand, within Psyr_0501at 545.246 kb on - strand, within Psyr_0501at 545.252 kb on - strand, within Psyr_0501at 545.309 kb on - strand, within Psyr_0501at 545.309 kb on - strand, within Psyr_0501at 545.309 kb on - strand, within Psyr_0501at 545.309 kb on - strand, within Psyr_0501at 545.461 kb on + strand, within Psyr_0501at 545.462 kb on - strand, within Psyr_0501at 545.578 kb on + strand, within Psyr_0501at 545.578 kb on + strand, within Psyr_0501at 545.578 kb on + strand, within Psyr_0501at 545.578 kb on + strand, within Psyr_0501at 545.579 kb on - strand, within Psyr_0501at 545.579 kb on - strand, within Psyr_0501at 545.579 kb on - strand, within Psyr_0501at 545.579 kb on - strand, within Psyr_0501at 545.579 kb on - strand, within Psyr_0501at 545.579 kb on - strand, within Psyr_0501at 545.579 kb on - strand, within Psyr_0501at 545.581 kb on + strand, within Psyr_0501at 545.581 kb on + strand, within Psyr_0501at 545.581 kb on + strand, within Psyr_0501at 545.581 kb on + strand, within Psyr_0501at 545.582 kb on - strand, within Psyr_0501at 545.582 kb on - strand, within Psyr_0501at 545.582 kb on - strand, within Psyr_0501at 545.582 kb on - strand, within Psyr_0501at 545.584 kb on + strand, within Psyr_0501at 545.584 kb on + strand, within Psyr_0501at 545.926 kb on + strand, within Psyr_0501at 545.926 kb on + strand, within Psyr_0501at 545.926 kb on + strand, within Psyr_0501at 545.927 kb on - strand, within Psyr_0501at 546.142 kb on + strand, within Psyr_0501at 546.168 kb on + strand, within Psyr_0501at 546.553 kb on + strand, within Psyr_0501at 546.553 kb on + strand, within Psyr_0501at 546.553 kb on + strand, within Psyr_0501at 546.554 kb on - strand, within Psyr_0501at 546.554 kb on - strand, within Psyr_0501at 546.554 kb on - strand, within Psyr_0501at 546.604 kb on + strand, within Psyr_0501at 546.605 kb on - strand, within Psyr_0501at 546.830 kb on - strand, within Psyr_0501at 547.348 kb on + strand, within Psyr_0501at 547.676 kb on + strandat 547.676 kb on + strandat 547.676 kb on + strandat 547.677 kb on - strandat 547.677 kb on - strandat 547.677 kb on - strandat 547.697 kb on + strandat 547.697 kb on + strandat 547.697 kb on + strandat 547.697 kb on + strandat 547.697 kb on + strandat 547.697 kb on + strandat 547.698 kb on - strandat 547.698 kb on - strandat 547.698 kb on - strandat 547.698 kb on - strandat 547.810 kb on + strandat 547.810 kb on + strandat 547.810 kb on + strandat 547.810 kb on + strandat 547.811 kb on - strandat 547.811 kb on - strandat 547.811 kb on - strandat 547.811 kb on - strandat 547.811 kb on - strandat 547.920 kb on + strand, within Psyr_0502at 548.086 kb on + strand, within Psyr_0502at 548.087 kb on - strand, within Psyr_0502at 548.087 kb on - strand, within Psyr_0502at 548.087 kb on - strand, within Psyr_0502at 548.237 kb on + strand, within Psyr_0502at 548.238 kb on - strand, within Psyr_0502at 548.317 kb on + strand, within Psyr_0502at 548.317 kb on + strand, within Psyr_0502at 548.370 kb on + strand, within Psyr_0502at 548.370 kb on + strand, within Psyr_0502at 548.370 kb on + strand, within Psyr_0502at 548.370 kb on + strand, within Psyr_0502at 548.371 kb on - strand, within Psyr_0502at 548.371 kb on - strand, within Psyr_0502at 548.371 kb on - strand, within Psyr_0502

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Per-strain Table

Position Strand Gene LocusTag Fraction KB with Nitrofurantoin 0.25 mg/ml
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543,293 + Psyr_0500 0.46 -1.2
543,294 - Psyr_0500 0.46 +0.2
543,294 - Psyr_0500 0.46 -0.3
543,550 + Psyr_0500 0.61 -0.8
543,551 - Psyr_0500 0.61 +0.3
543,551 - Psyr_0500 0.61 +0.0
543,558 + Psyr_0500 0.61 +0.6
543,793 + Psyr_0500 0.75 -0.6
543,794 - Psyr_0500 0.75 +0.6
544,188 - +1.8
544,343 + +0.4
544,420 + +1.1
544,447 + -0.3
544,447 + +1.3
544,447 + -0.2
544,447 + -1.4
544,448 - +1.3
544,448 - -0.1
544,448 - -0.6
544,554 + -0.6
544,555 - -1.5
544,555 - -0.2
544,555 - +1.1
544,555 - +0.3
544,555 - +1.2
544,584 - -1.2
544,606 + -0.4
544,659 + Psyr_0501 0.11 +0.6
544,659 + Psyr_0501 0.11 -0.3
544,660 - Psyr_0501 0.11 -1.8
545,057 - Psyr_0501 0.23 +0.1
545,057 - Psyr_0501 0.23 -0.0
545,080 + Psyr_0501 0.23 +0.6
545,121 + Psyr_0501 0.24 -0.1
545,122 - Psyr_0501 0.25 +1.8
545,245 + Psyr_0501 0.28 +0.1
545,245 + Psyr_0501 0.28 -1.2
545,246 - Psyr_0501 0.28 +1.5
545,252 - Psyr_0501 0.28 -0.3
545,309 - Psyr_0501 0.30 +1.8
545,309 - Psyr_0501 0.30 -1.4
545,309 - Psyr_0501 0.30 +1.0
545,309 - Psyr_0501 0.30 -0.0
545,461 + Psyr_0501 0.34 +0.1
545,462 - Psyr_0501 0.35 -0.3
545,578 + Psyr_0501 0.38 +0.5
545,578 + Psyr_0501 0.38 -0.3
545,578 + Psyr_0501 0.38 +0.0
545,578 + Psyr_0501 0.38 -0.5
545,579 - Psyr_0501 0.38 -0.4
545,579 - Psyr_0501 0.38 -0.3
545,579 - Psyr_0501 0.38 +1.1
545,579 - Psyr_0501 0.38 -1.5
545,579 - Psyr_0501 0.38 -0.0
545,579 - Psyr_0501 0.38 -0.6
545,579 - Psyr_0501 0.38 +0.1
545,581 + Psyr_0501 0.38 -0.0
545,581 + Psyr_0501 0.38 -0.6
545,581 + Psyr_0501 0.38 -0.0
545,581 + Psyr_0501 0.38 +0.3
545,582 - Psyr_0501 0.38 -0.4
545,582 - Psyr_0501 0.38 -1.0
545,582 - Psyr_0501 0.38 -0.2
545,582 - Psyr_0501 0.38 +0.1
545,584 + Psyr_0501 0.38 -0.1
545,584 + Psyr_0501 0.38 -0.3
545,926 + Psyr_0501 0.48 -0.8
545,926 + Psyr_0501 0.48 -0.6
545,926 + Psyr_0501 0.48 -1.1
545,927 - Psyr_0501 0.48 -0.2
546,142 + Psyr_0501 0.54 -0.1
546,168 + Psyr_0501 0.55 +1.3
546,553 + Psyr_0501 0.67 -0.6
546,553 + Psyr_0501 0.67 -0.8
546,553 + Psyr_0501 0.67 -1.5
546,554 - Psyr_0501 0.67 +1.9
546,554 - Psyr_0501 0.67 -0.8
546,554 - Psyr_0501 0.67 +0.3
546,604 + Psyr_0501 0.68 -0.0
546,605 - Psyr_0501 0.68 +0.1
546,830 - Psyr_0501 0.75 -1.0
547,348 + Psyr_0501 0.90 -0.4
547,676 + -0.2
547,676 + -0.2
547,676 + -0.0
547,677 - -0.6
547,677 - -1.1
547,677 - -0.5
547,697 + +1.8
547,697 + +1.0
547,697 + +0.3
547,697 + -0.8
547,697 + -2.8
547,697 + +0.9
547,698 - -0.3
547,698 - -0.6
547,698 - +0.8
547,698 - -0.3
547,810 + -2.4
547,810 + +0.7
547,810 + -0.8
547,810 + +4.0
547,811 - +1.8
547,811 - -0.4
547,811 - -0.5
547,811 - -0.3
547,811 - +2.8
547,920 + Psyr_0502 0.11 +0.6
548,086 + Psyr_0502 0.21 +0.5
548,087 - Psyr_0502 0.21 -2.5
548,087 - Psyr_0502 0.21 +1.2
548,087 - Psyr_0502 0.21 -0.2
548,237 + Psyr_0502 0.29 -0.2
548,238 - Psyr_0502 0.29 +0.7
548,317 + Psyr_0502 0.34 -0.5
548,317 + Psyr_0502 0.34 +0.6
548,370 + Psyr_0502 0.36 +0.4
548,370 + Psyr_0502 0.36 +0.2
548,370 + Psyr_0502 0.36 +0.9
548,370 + Psyr_0502 0.36 -0.2
548,371 - Psyr_0502 0.37 +0.2
548,371 - Psyr_0502 0.37 +1.1
548,371 - Psyr_0502 0.37 +0.3

Or see this region's nucleotide sequence