Experiment: KB with Nitrofurantoin 0.25 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_0451 and Psyr_0452 are separated by 122 nucleotides Psyr_0452 and Psyr_0453 are separated by 106 nucleotides Psyr_0453 and Psyr_0454 are separated by 131 nucleotides
Psyr_0451: Psyr_0451 - regulatory protein, LysR:LysR, substrate-binding protein, at 486,927 to 487,853
_0451
Psyr_0452: Psyr_0452 - HylII, at 487,976 to 488,590
_0452
Psyr_0453: Psyr_0453 - 16S rRNA m(3)U-1498 methyltransferase, at 488,697 to 489,416
_0453
Psyr_0454: Psyr_0454 - adenosylmethionine-8-amino-7-oxononanoate aminotransferase apoenzyme, at 489,548 to 490,954
_0454
Position (kb) 488
489
490 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 487.752 kb on - strand, within Psyr_0451 at 487.752 kb on - strand, within Psyr_0451 at 487.813 kb on - strand at 487.813 kb on - strand at 487.865 kb on + strand at 487.872 kb on - strand at 487.872 kb on - strand at 487.894 kb on + strand at 487.894 kb on + strand at 487.904 kb on + strand at 487.904 kb on + strand at 487.904 kb on + strand at 487.904 kb on + strand at 487.904 kb on + strand at 487.904 kb on + strand at 487.904 kb on + strand at 487.904 kb on + strand at 487.904 kb on + strand at 487.905 kb on - strand at 487.905 kb on - strand at 487.905 kb on - strand at 487.905 kb on - strand at 487.905 kb on - strand at 487.905 kb on - strand at 487.905 kb on - strand at 487.905 kb on - strand at 487.905 kb on - strand at 487.905 kb on - strand at 487.905 kb on - strand at 487.905 kb on - strand at 487.905 kb on - strand at 487.905 kb on - strand at 487.947 kb on + strand at 487.947 kb on + strand at 487.947 kb on + strand at 487.948 kb on - strand at 487.956 kb on + strand at 487.969 kb on + strand at 487.979 kb on + strand at 487.979 kb on + strand at 487.979 kb on + strand at 487.979 kb on + strand at 487.979 kb on + strand at 487.979 kb on + strand at 487.979 kb on + strand at 487.980 kb on - strand at 487.980 kb on - strand at 487.980 kb on - strand at 487.980 kb on - strand at 487.982 kb on + strand at 488.108 kb on + strand, within Psyr_0452 at 488.108 kb on + strand, within Psyr_0452 at 488.108 kb on + strand, within Psyr_0452 at 488.108 kb on + strand, within Psyr_0452 at 488.108 kb on + strand, within Psyr_0452 at 488.108 kb on + strand, within Psyr_0452 at 488.108 kb on + strand, within Psyr_0452 at 488.108 kb on + strand, within Psyr_0452 at 488.108 kb on + strand, within Psyr_0452 at 488.108 kb on + strand, within Psyr_0452 at 488.108 kb on + strand, within Psyr_0452 at 488.108 kb on + strand, within Psyr_0452 at 488.109 kb on - strand, within Psyr_0452 at 488.109 kb on - strand, within Psyr_0452 at 488.109 kb on - strand, within Psyr_0452 at 488.109 kb on - strand, within Psyr_0452 at 488.109 kb on - strand, within Psyr_0452 at 488.109 kb on - strand, within Psyr_0452 at 488.109 kb on - strand, within Psyr_0452 at 488.109 kb on - strand, within Psyr_0452 at 488.109 kb on - strand, within Psyr_0452 at 488.109 kb on - strand, within Psyr_0452 at 488.109 kb on - strand, within Psyr_0452 at 488.109 kb on - strand, within Psyr_0452 at 488.109 kb on - strand, within Psyr_0452 at 488.109 kb on - strand, within Psyr_0452 at 488.109 kb on - strand, within Psyr_0452 at 488.109 kb on - strand, within Psyr_0452 at 488.109 kb on - strand, within Psyr_0452 at 488.132 kb on + strand, within Psyr_0452 at 488.133 kb on - strand, within Psyr_0452 at 488.133 kb on - strand, within Psyr_0452 at 488.133 kb on - strand, within Psyr_0452 at 488.150 kb on + strand, within Psyr_0452 at 488.210 kb on + strand, within Psyr_0452 at 488.211 kb on - strand, within Psyr_0452 at 488.211 kb on - strand, within Psyr_0452 at 488.211 kb on - strand, within Psyr_0452 at 488.211 kb on - strand, within Psyr_0452 at 488.211 kb on - strand, within Psyr_0452 at 488.231 kb on + strand, within Psyr_0452 at 488.231 kb on + strand, within Psyr_0452 at 488.231 kb on + strand, within Psyr_0452 at 488.231 kb on + strand, within Psyr_0452 at 488.232 kb on - strand, within Psyr_0452 at 488.232 kb on - strand, within Psyr_0452 at 488.328 kb on + strand, within Psyr_0452 at 488.328 kb on + strand, within Psyr_0452 at 488.329 kb on - strand, within Psyr_0452 at 488.369 kb on + strand, within Psyr_0452 at 488.370 kb on - strand, within Psyr_0452 at 488.370 kb on - strand, within Psyr_0452 at 488.468 kb on + strand, within Psyr_0452 at 488.468 kb on + strand, within Psyr_0452 at 488.468 kb on + strand, within Psyr_0452 at 488.468 kb on + strand, within Psyr_0452 at 488.469 kb on - strand, within Psyr_0452 at 488.579 kb on + strand at 488.579 kb on + strand at 488.579 kb on + strand at 488.580 kb on - strand at 488.580 kb on - strand at 489.098 kb on + strand, within Psyr_0453 at 489.099 kb on - strand, within Psyr_0453 at 489.099 kb on - strand, within Psyr_0453 at 489.340 kb on + strand, within Psyr_0453 at 489.340 kb on + strand, within Psyr_0453 at 489.340 kb on + strand, within Psyr_0453 at 489.340 kb on + strand, within Psyr_0453 at 489.340 kb on + strand, within Psyr_0453 at 489.340 kb on + strand, within Psyr_0453 at 489.340 kb on + strand, within Psyr_0453 at 489.340 kb on + strand, within Psyr_0453 at 489.341 kb on - strand, within Psyr_0453 at 489.341 kb on - strand, within Psyr_0453 at 489.341 kb on - strand, within Psyr_0453 at 489.341 kb on - strand, within Psyr_0453 at 489.341 kb on - strand, within Psyr_0453 at 489.341 kb on - strand, within Psyr_0453 at 489.549 kb on + strand at 489.678 kb on + strand at 489.679 kb on - strand at 489.679 kb on - strand at 489.694 kb on - strand, within Psyr_0454 at 489.694 kb on - strand, within Psyr_0454 at 489.694 kb on - strand, within Psyr_0454 at 489.694 kb on - strand, within Psyr_0454 at 489.694 kb on - strand, within Psyr_0454 at 489.991 kb on - strand, within Psyr_0454 at 489.991 kb on - strand, within Psyr_0454 at 489.991 kb on - strand, within Psyr_0454 at 489.991 kb on - strand, within Psyr_0454 at 490.035 kb on + strand, within Psyr_0454 at 490.035 kb on + strand, within Psyr_0454 at 490.048 kb on - strand, within Psyr_0454 at 490.087 kb on - strand, within Psyr_0454 at 490.087 kb on - strand, within Psyr_0454 at 490.245 kb on + strand, within Psyr_0454 at 490.245 kb on + strand, within Psyr_0454 at 490.257 kb on + strand, within Psyr_0454 at 490.257 kb on + strand, within Psyr_0454 at 490.257 kb on + strand, within Psyr_0454 at 490.258 kb on - strand, within Psyr_0454 at 490.258 kb on - strand, within Psyr_0454 at 490.258 kb on - strand, within Psyr_0454 at 490.258 kb on - strand, within Psyr_0454 at 490.258 kb on - strand, within Psyr_0454 at 490.258 kb on - strand, within Psyr_0454 at 490.402 kb on - strand, within Psyr_0454
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Nitrofurantoin 0.25 mg/ml remove 487,752 - Psyr_0451 0.89 +0.9 487,752 - Psyr_0451 0.89 +0.5 487,813 - -0.2 487,813 - -0.2 487,865 + +0.4 487,872 - +0.0 487,872 - +0.6 487,894 + +0.3 487,894 + -0.1 487,904 + -0.5 487,904 + -0.1 487,904 + -0.3 487,904 + +0.9 487,904 + -0.4 487,904 + -0.5 487,904 + -0.9 487,904 + +0.6 487,904 + +0.6 487,905 - +0.1 487,905 - +0.3 487,905 - -0.4 487,905 - +0.6 487,905 - -0.8 487,905 - +0.3 487,905 - +0.1 487,905 - +0.9 487,905 - -0.5 487,905 - +1.3 487,905 - +0.0 487,905 - +0.0 487,905 - -0.1 487,905 - +2.7 487,947 + +1.1 487,947 + +0.0 487,947 + +0.1 487,948 - +0.1 487,956 + -0.5 487,969 + +0.5 487,979 + -0.8 487,979 + -0.1 487,979 + +2.1 487,979 + +0.4 487,979 + -2.1 487,979 + +1.2 487,979 + -1.5 487,980 - +0.3 487,980 - +0.0 487,980 - -0.7 487,980 - -0.9 487,982 + +2.4 488,108 + Psyr_0452 0.21 +0.9 488,108 + Psyr_0452 0.21 +0.6 488,108 + Psyr_0452 0.21 -1.4 488,108 + Psyr_0452 0.21 -0.0 488,108 + Psyr_0452 0.21 +0.7 488,108 + Psyr_0452 0.21 -2.1 488,108 + Psyr_0452 0.21 +0.3 488,108 + Psyr_0452 0.21 -0.7 488,108 + Psyr_0452 0.21 +0.1 488,108 + Psyr_0452 0.21 +0.0 488,108 + Psyr_0452 0.21 -2.7 488,108 + Psyr_0452 0.21 +1.4 488,109 - Psyr_0452 0.22 -2.0 488,109 - Psyr_0452 0.22 -0.7 488,109 - Psyr_0452 0.22 +0.4 488,109 - Psyr_0452 0.22 +0.6 488,109 - Psyr_0452 0.22 -1.6 488,109 - Psyr_0452 0.22 -0.9 488,109 - Psyr_0452 0.22 -0.3 488,109 - Psyr_0452 0.22 -0.0 488,109 - Psyr_0452 0.22 -0.6 488,109 - Psyr_0452 0.22 -1.0 488,109 - Psyr_0452 0.22 -0.8 488,109 - Psyr_0452 0.22 +0.6 488,109 - Psyr_0452 0.22 +0.4 488,109 - Psyr_0452 0.22 +0.3 488,109 - Psyr_0452 0.22 -1.1 488,109 - Psyr_0452 0.22 +0.4 488,109 - Psyr_0452 0.22 +1.6 488,132 + Psyr_0452 0.25 -0.9 488,133 - Psyr_0452 0.26 +0.4 488,133 - Psyr_0452 0.26 -0.1 488,133 - Psyr_0452 0.26 +0.5 488,150 + Psyr_0452 0.28 -0.4 488,210 + Psyr_0452 0.38 -1.6 488,211 - Psyr_0452 0.38 -0.7 488,211 - Psyr_0452 0.38 -0.6 488,211 - Psyr_0452 0.38 +0.9 488,211 - Psyr_0452 0.38 -0.3 488,211 - Psyr_0452 0.38 -1.7 488,231 + Psyr_0452 0.41 -0.9 488,231 + Psyr_0452 0.41 -1.5 488,231 + Psyr_0452 0.41 -0.3 488,231 + Psyr_0452 0.41 -0.3 488,232 - Psyr_0452 0.42 +0.3 488,232 - Psyr_0452 0.42 +0.6 488,328 + Psyr_0452 0.57 +1.4 488,328 + Psyr_0452 0.57 -0.1 488,329 - Psyr_0452 0.57 -0.9 488,369 + Psyr_0452 0.64 -0.2 488,370 - Psyr_0452 0.64 -1.5 488,370 - Psyr_0452 0.64 -0.1 488,468 + Psyr_0452 0.80 +0.2 488,468 + Psyr_0452 0.80 +1.0 488,468 + Psyr_0452 0.80 -0.6 488,468 + Psyr_0452 0.80 -0.7 488,469 - Psyr_0452 0.80 -0.4 488,579 + +0.1 488,579 + -0.7 488,579 + +0.3 488,580 - +1.8 488,580 - +0.9 489,098 + Psyr_0453 0.56 +0.9 489,099 - Psyr_0453 0.56 +2.1 489,099 - Psyr_0453 0.56 -0.1 489,340 + Psyr_0453 0.89 +0.6 489,340 + Psyr_0453 0.89 +0.4 489,340 + Psyr_0453 0.89 +2.3 489,340 + Psyr_0453 0.89 +0.2 489,340 + Psyr_0453 0.89 +0.6 489,340 + Psyr_0453 0.89 +0.1 489,340 + Psyr_0453 0.89 +1.9 489,340 + Psyr_0453 0.89 +0.5 489,341 - Psyr_0453 0.89 +1.4 489,341 - Psyr_0453 0.89 +0.4 489,341 - Psyr_0453 0.89 -0.7 489,341 - Psyr_0453 0.89 -1.0 489,341 - Psyr_0453 0.89 +0.2 489,341 - Psyr_0453 0.89 +1.2 489,549 + +0.2 489,678 + -2.6 489,679 - -2.7 489,679 - -1.1 489,694 - Psyr_0454 0.10 -2.1 489,694 - Psyr_0454 0.10 -2.1 489,694 - Psyr_0454 0.10 -0.5 489,694 - Psyr_0454 0.10 -0.1 489,694 - Psyr_0454 0.10 -3.5 489,991 - Psyr_0454 0.31 -1.1 489,991 - Psyr_0454 0.31 +0.9 489,991 - Psyr_0454 0.31 -3.3 489,991 - Psyr_0454 0.31 -1.7 490,035 + Psyr_0454 0.35 -1.5 490,035 + Psyr_0454 0.35 -1.1 490,048 - Psyr_0454 0.36 -0.5 490,087 - Psyr_0454 0.38 -0.6 490,087 - Psyr_0454 0.38 -2.6 490,245 + Psyr_0454 0.50 -1.5 490,245 + Psyr_0454 0.50 -1.7 490,257 + Psyr_0454 0.50 -1.5 490,257 + Psyr_0454 0.50 +1.1 490,257 + Psyr_0454 0.50 -1.5 490,258 - Psyr_0454 0.50 -1.7 490,258 - Psyr_0454 0.50 -2.7 490,258 - Psyr_0454 0.50 -0.6 490,258 - Psyr_0454 0.50 -2.5 490,258 - Psyr_0454 0.50 -2.5 490,258 - Psyr_0454 0.50 -2.3 490,402 - Psyr_0454 0.61 +0.0
Or see this region's nucleotide sequence